Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome

Applied and Environmental Microbiology

Volumn 74, Issue 13, 2008, Pages 4241-4245

  Sun, Wei ^a   Wang, Shifeng ^a   Curtiss III, Roy ^a  

^a ARIZONA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMERICAN SOCIETY; GENE DELETION; GENETIC MODIFICATION (GM); HIGHLY EFFICIENT; LIVE ATTENUATED VACCINES (LAV); MARKERLESS; Y. PESTIS; YERSINIA PESTIS;

GENETIC ENGINEERING;

LIVE VACCINE;

BACTERIUM; BIOTECHNOLOGY; CHROMOSOME; RECOMBINATION; VACCINE;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL VIRULENCE; DNA MODIFICATION; GENE DELETION; GENE EXPRESSION; GENE INSERTION; GENETIC RECOMBINATION; MARKER GENE; NONHUMAN; SACB GENE; SPOT GENE; YERSINIA PESTIS;

BACTERIAL PROTEINS; CHROMOSOMES, BACTERIAL; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; GENETIC MARKERS; MUTAGENESIS, INSERTIONAL; RECOMBINATION, GENETIC; YERSINIA PESTIS;

YERSINIA PESTIS;

EID: 46949111513     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.00940-08     Document Type: Article

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