Efficient and precise construction of markerless manipulations in the Bacillus subtilis genome

Journal of Microbiology and Biotechnology

Volumn 20, Issue 1, 2010, Pages 45-53

  Yu, Haojie ^a   Xin, Yan ^a   Weiliang, Shen ^a   Yujia, Shen ^a   Ji, Zhang ^a   Shunpeng, Li ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

Bacillus subtilis; High fidelity fusion PCR; Markless genome manipulation; mazF

Indexed keywords

BACTERIAL TOXIN; HISTIDINE; PRIMER DNA; REPETITIVE DNA; XYLOSE;

AMYE GENE; ANTIBIOTIC RESISTANCE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL STRAIN; ESCHERICHIA COLI; GENE CASSETTE; GENE CONSTRUCT; GENE DELETION; GENE EXPRESSION SYSTEM; GENE INACTIVATION; GENE LOCUS; GENE SEQUENCE; GENE TARGETING; HISZ GENE; HOMOLOGOUS RECOMBINATION; MAZF CASSETTE; MAZF GENE; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PLASMID; POINT MUTATION; POLYMERASE CHAIN REACTION; PROPHAGE; R FACTOR; RNA SPLICING; STRUCTURAL HOMOLOGY;

BACILLUS SUBTILIS; DNA-BINDING PROTEINS; ENDORIBONUCLEASES; ESCHERICHIA COLI PROTEINS; GENE TARGETING; GENOME, BACTERIAL; MUTATION; RECOMBINATION, GENETIC;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 75849156352     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.0904.04051     Document Type: Article

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