High-efficiency scarless genetic modification in escherichia coli by using lambda red recombination and I-SceI cleavage

Applied and Environmental Microbiology

Volumn 80, Issue 13, 2014, Pages 3826-3834

  Yang, Junjie ^a,b   Sun, Bingbing ^a,b   Huang, He ^a,b   Jiang, Yu ^a,b   Diao, Liuyang ^a,b   Chen, Biao ^a,b   Xu, Chongmao ^a,b   Wang, Xin ^a,c   Liu, Jinle ^a,c   Jiang, Weihong ^a   Yang, Sheng ^a,b  

^a INSTITUTE OF PLANT PHYSIOLOGY AND ECOLOGY   (China)

^b Shanghai Research and Development Center of Industrial Biotechnology   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; ESCHERICHIA COLI; GENETIC ENGINEERING; POLYMERASE CHAIN REACTION;

BACTERIAL CHROMOSOMES; DOUBLE STRANDED DNA; EASY-TO-USE SYSTEMS; GENE MODIFICATION; GENETIC MODIFICATIONS; S-ADENOSYLMETHIONINE; SITE DIRECTED MUTAGENESIS; TRANSPORTER GENES;

GENES;

CHROMOSOME; COLIFORM BACTERIUM; ENZYME ACTIVITY; GENETIC MARKER; GENETICALLY MODIFIED ORGANISM; RECOMBINATION;

ENDONUCLEASE; RECOMBINASE;

ESCHERICHIA COLI; GENETIC RECOMBINATION; GENETICS; MICROBIAL GENETICS; MOLECULAR BIOLOGY; PLASMID; PROCEDURES;

ENDONUCLEASES; ESCHERICHIA COLI; GENETICS, MICROBIAL; MOLECULAR BIOLOGY; PLASMIDS; RECOMBINASES; RECOMBINATION, GENETIC;

EID: 84902147306     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00313-14     Document Type: Article

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