Biomolecular Deuteration for Neutron Structural Biology and Dynamics

Methods in Enzymology

Volumn 566, Issue , 2016, Pages 113-157

  Haertlein, Michael ^a   Moulin, Martine ^a   Devos, Juliette M ^a   Laux, Valerie ^a   Dunne, Orla ^a,b   Trevor Forsyth, V ^a,c  

^a INSTITUT LAUE LANGEVIN   (France)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

^c KEELE UNIVERSITY   (United Kingdom)

Author keywords

Contrast variation; Deuteration; Dynamics; Isotope labelling; Neutron crystallography; Neutron reflection; Neutron scattering; SANS; Structural biology

Indexed keywords

AMINO ACID; DEUTERIUM; DNA; LIPID; MEMBRANE PROTEIN; RECOMBINANT PROTEIN; TRANSFER RNA; SOLUTION AND SOLUBILITY;

ARTICLE; BIOREACTOR; CELL COMPONENT; CELL CULTURE; CELL FREE SYSTEM; CHEMICAL PROCEDURES; CHEMICAL STRUCTURE; CRYSTALLOGRAPHY; CULTURE MEDIUM; CYANOBACTERIUM; ESCHERICHIA COLI; EUKARYOTE; GENE EXPRESSION SYSTEM; HUMAN; ISOTOPE LABELING; MACROMOLECULAR DEUTERATION; MACROMOLECULE; MOLECULAR DYNAMICS; NEUTRON; NEUTRON REFLECTION TECHNIQUE; NEUTRON SCATTERING; NONHUMAN; PERDEUTERATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; X RAY DIFFRACTION; CHEMISTRY; MOLECULAR BIOLOGY; PROCEDURES; SMALL ANGLE SCATTERING; SOLUTION AND SOLUBILITY; STAINING;

CRYSTALLOGRAPHY; DEUTERIUM; HUMANS; MOLECULAR BIOLOGY; NEUTRONS; SCATTERING, SMALL ANGLE; SOLUTIONS; STAINING AND LABELING; X-RAY DIFFRACTION;

EID: 84954392033     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/bs.mie.2015.11.001     Document Type: Chapter

References (139)

1. K. Achterhold, A. Ostermann, M. Moulin, M. Haertlein, T. Unruh, G. Fritz, and et al. Dynamical properties of the hydration shell of fully deuterated myoglobin Physical Review E 84 2011 041930
2. A. Appolaire, E. Girard, M. Colombo, M.A. Durá, M. Moulin, M. Haertlein, and et al. Small-angle neutron scattering reveals the assembly mode and oligomeric architecture of TET, a large, dodecameric aminopeptidase Acta Crystallographica D 70 Pt. 11 2014 2983 2993
3. 2O Acta Crystallographica. Section D, Biological Crystallography 61 Pt. 11 2005 1541 1549
4. I. Ayala, R. Sounier, N. Usé, P. Gans, and J. Boisbouvier An efficient protocol for the complete incorporation of methyl-protonated alanine in perdeuterated protein Journal of Biomolecular NMR 43 2 2009 111 119
5. F. Baneys Recombinant protein expression in E. coli Current Opinion in Biotechnology 10 1999 411 421
6. P.H. Bessette, F. Aslund, J. Beckwith, and G. Georgiou Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm Proceedings of the National Academy of Sciences of the United States of America 96 24 1999 13703 13708
7. M.P. Blakeley Neutron macromolecular crystallography Crystallography Reviews 15 2009 157 218
8. M.P. Blakeley, F. Ruiz, R. Cachau, I. Hazemann, F. Meilleur, A. Mitschler, and et al. Quantum model of catalysis based on a mobile proton revealed by subatomic X-ray and neutron diffraction studies of h-aldose reductase Proceedings of the National Academy of Sciences of the United States of America 105 6 2008 1844 1848
9. M.P. Blakeley, S.C. Teixeira, I. Petit-Haertlein, I. Hazemann, A. Mitschler, M. Haertlein, and et al. Neutron macromolecular crystallography with LADI-III Acta Crystallographica D 66 2010 1198 1205
10. C. Breyton, F. Gabel, M. Lethier, A. Flayhan, G. Durand, J.M. Jault, and et al. Small angle neutron scattering for the study of solubilised membrane proteins The European Physical Journal E, Soft Matter 36 7 2013 13071 13076
11. F. Bringezu, S. Wen, S. Dante, T. Hauss, M. Majerowicz, and A. Waring The insertion of the antimicrobial peptide dicynthaurin monomer in model membranes: Thermodynamics and structural characterization Biochemistry 46 19 2007 5678 5686
12. D. Brockwell, L. Yu, S. Cooper, S. McCleland, A. Cooper, D. Attwood, and et al. Physicochemical consequences of the perdeuteriation of glutathione S-transferase from S. japonicum Protein Science 10 3 2001 572 580
13. N. Brouette, G. Fragneto, F. Cousin, M. Moulin, M. Haertlein, and M. Sferrazza A neutron reflection study of adsorbed deuterated myoglobin layers on hydrophobic surfaces Journal of Colloid and Interface Science 390 1 2013 114 120
14. P. Callow, A. Sukhodub, J. Taylor, and G. Kneale Shape and subunit organisation of the DNA methyltransferase M.Ahdl Journal of Molecular Biology 69 1 2007 177 185
15. M.S. Capel, D.M. Engelman, B.R. Freeborn, M. Kjeldgaard, J.A. Langer, V. Ramakrishnan, and et al. A complete mapping of the proteins in the small ribosomal subunit of Escherichia coli Science 238 1987 1403 1406
16. B.C. Carlstedt, H.L. Crespi, M.I. Blake, and J.J. Katz Biosynthesis of deuterated benzylpenicillins. I. Solvent deuterium oxide participation Journal of Pharmaceutical Sciences 59 10 1970 1456 1460
17. C. Casadei, A. Gumiero, C.L. Metcalfe, E.J. Murphy, J. Basran, M.G. Concilio, and et al. Heme enzymes. Neutron cryo-crystallography captures the protonation state of ferryl heme in a peroxidase Science 345 2014 193 197
18. W.C. Chan, and P.D. White FMOC solid phase peptide synthesis: A practical approach 2000 Oxford University Press New York, NY
19. A. Chenal, L. Prongidi-Fix, A. Perier, C. Aisenbrey, G. Vernier, S. Lambotte, and et al. Deciphering membrane insertion of the diphtheria toxin T domain by specular neutron reflectometry and solid-state NMR spectroscopy Journal of Molecular Biology 391 5 2009 872 883
20. M.J. Clarke, J.-B. Artero, M. Moulin, P. Callow, J.A. Carver, P.C. Griffiths, and et al. Investigation of gamma E-crystallin target protein binding to bovine lens alpha-crystallin by small-angle neutron scattering Biochimica et Biophysica Acta 1800 3 2010 392 397
21. E.L. Compton, K. Page, H.E. Findlay, M. Haertlein, M. Moulin, U. Zachariae, and et al. Conserved structure and domain organization among bacterial Slc26 transporters The Biochemical Journal 463 2 2014 297 307
22. B.J. Cowsill, X. Zhao, T.A. Waigh, S. Eapen, R. Davies, V. Laux, and et al. Interfacial structure of immobilized antibodies and perdeuterated HSA in model pregnancy tests measured with neutron reflectivity Langmuir 30 20 2014 5880 5887
23. J.M. Cregg, and D.R. Higgins Production of foreign proteins in the yeast Pichia pastoris Canadian Journal of Botany 73 Suppl. 1 1995 S891 S897
24. M.G. Cuypers, S.A. Mason, M.P. Blakeley, E.P. Mitchell, M. Haertlein, and V.T. Forsyth Near-atomic resolution neutron crystallography on perdeuterated Pyrococcus furiosus rubredoxin: Implication of hydronium ions and protonation state equilibria in redox changes Angewandte Chemie 52 3 2013 1022 1025
25. M.G. Cuypers, M. Trubitsyna, P. Callow, V.T. Forsyth, and J.M. Richardson Solution conformations of early intermediates in Mos1 transposition Nucleic Acids Research 41 3 2013 2020 2033
26. A. de Ghellinck, G. Fragneto, V. Laux, M. Haertlein, J. Jouhet, M. Michele Sferrazza, and et al. Lipid polyunsaturation determines the extent of membrane structural changes induced by Amphotericin B in Pichia pastoris yeast, BBA Biomembranes 1848 2015 2317 2325
27. A. de Ghellinck, H. Schaller, V. Laux, M. Haertlein, M. Sferrazza, E. Maréchal, and et al. Production and analysis of perdeuterated lipids from Pichia pastoris cells PLoS One 9 4 2014 e92999
28. I.G. Denisov, Y.V. Grinkova, A.A. Lazarides, and S.G. Sligar Directed self-assembly of monodisperse phospholipid bilayer nanodiscs with controlled size Journal of the American Chemical Society 126 11 2004 3477 3487
29. 15N-labeling of the 24-kDa N-terminal domain of the Escherichia coli gyrase B by overexpression in the photoautotrophic cyanobacterium Anabaena sp. PCC 7120 Protein Expression and Purification 23 1 2001 207 217
30. L. Di Costanzo, M. Moulin, M. Haertlein, F. Meilleur, and D.W. Christianson Expression, purification, assay, and crystal structure of perdeuterated human arginase Archives of Biochemistry and Biophysics 465 1 2007 82 89
31. Dunne, O., Moulin, M., Callow, P., Martel, A., Weidenhaupt, M., Perkins, S. M., et al. Matchout deuterium labelling of proteins using prokaryotic and eukaryotic expression systems. European Biophysics Journal. (submitted).
32. C. Edlich-Muth, J.-B. Artero, P. Callow, M.R. Przewloka, A.A. Watson, W. Zhang, and et al. The pentaplasmic nucleoplasmin fold is present in Drosophila FKBP39 and a large number of chromatin-related proteins Journal of Molecular Biology 427 10 2015 1949 1963
33. T. Etezady-Esfarjani, S. Hiller, C. Villalba, and K. Wüthrich Cell-free protein synthesis of perdeuterated proteins for NMR studies Journal of Biomolecular NMR 39 3 2007 229 238
34. Y. Fichou, G. Schirò, F.X. Gallat, C. Laguri, M. Moulin, J. Combet, and et al. Hydration water mobility is enhanced around tau amyloid fibers Proceedings of the National Academy of Sciences of the United States of America 112 20 2015 6365 6370
35. S.J. Fisher, M.P. Blakeley, E.I. Howard, I. Petit-Haertlein, M. Haertlein, A. Mitschler, and et al. Perdeuteration: Improved visualization of solvent structure in neutron macromolecular crystallography Acta Crystallographica D 70 2014 3266 3272
36. S.J. Fisher, and J.R. Helliwell An investigation into structural changes due to deuteration Acta Crystallographica A64 Pt. 3 2008 359 367
37. V.T. Forsyth, R.J. Greenall, R. Hussain, A. Mahendrasingam, C. Nave, W.J. Pigram, and et al. X-ray high-angle fibre diffraction studies of nucleic acid structure using the Daresbury synchrotron radiation source Biochemical Society Transactions 14 1986 553 557
38. V.T. Forsyth, A. Mahendrasingam, W.J. Pigram, R.J. Greenall, K.A. Bellamy, W. Fuller, and et al. Neutron fibre diffraction studies of DNA hydration International Journal of Biological Macromolecules 11 1989 236 240
39. V.T. Forsyth, D. Myles, P.A. Timmins, and M. Haertlein A laboratory to support the deuteration of biological macromolecules Report to the ILL millennium programme 2001 32 34
40. W. Fuller, V.T. Forsyth, and A. Mahendrasingam Water-DNA interactions by X-ray and neutron fibre diffraction Philosophical Transactions of the Royal Society 359 1448 2004 1237 1248
41. F. Gabel, D. Bicout, U. Lehnert, M. Tehei, M. Weik, and G. Zaccai Protein dynamics studied by neutron scattering Quarterly Reviews of Biophysics 35 4 2002 327 367
42. F.X. Gallat, A.P. Brogan, Y. Fichou, N. McGrath, M. Moulin, M. Härtlein, and et al. A polymer surfactant corona dynamically replaces water in solvent-free protein liquids and ensures macromolecular flexibility and activity Journal of the American Chemical Society 134 32 2012 13168 13171
43. F.X. Gallat, A. Laganowsky, K. Wood, F. Gabel, L. van Eijck, J. Wuttke, and et al. Dynamical coupling of intrinsically disordered proteins and their hydration water: Comparison with folded soluble and membrane proteins Biophysical Journal 103 1 2012 129 136
44. T.R. Gamble, K.R. Clauser, and A.A. Kossiakoff The production and X-ray structure determination of perdeuterated Staphylococcal nuclease Biophysical Chemistry 53 1-2 1994 15 25
45. K.H. Gardner, A. English, and V.T. Forsyth New insights into the structure of poly(p-phenylene terephthalamide) from neutron fiber diffraction studies Macromolecules 37 2004 9654 9656
46. Y. Gerelli, A. de Ghellinck, J. Jouhet, V. Laux, M. Haertlein, and G. Fragneto Multi-lamellar organization of fully deuterated lipid extracts of yeast membranes Acta Crystallographica D 70 2014 3167 3176
47. P.G. Gettins, M. Backovic, and F.C. Peterson Use of NMR to study serpin function Methods 32 2 2004 120 129
48. S.L. Grage, A.M. Keleshian, T. Turdzeladze, A.R. Battle, W.C. Tay, R.P. May, and et al. Bilayer-mediated clustering and functional interaction of MscL channels Biophysical Journal 100 5 2011 1252 1260
49. J.G. Grossmann, A.J. Callaghan, M.J. Marcaida, B.F. Luisi, F.H. Alcock, K. Tokatlidis, and et al. Complementing structural information of modular proteins with small angle neutron scattering and contrast variation European Biophysics Journal 37 2008 719
50. F. Hagn, M. Etzkorn, T. Raschle, and G. Wagner Optimized phospholipid bilayer nanodiscs facilitate high-resolution structure determination of membrane proteins Journal of the American Chemical Society 135 5 2013 1919 1925
51. S. Haon, S. Auge, M. Tropis, A. Milon, and N.D. Lindley Low cost production of perdeuterated biomass using methylothrophic yeasts Journal of Labelled Compounds and Radiopharmaceuticals XXXIII 11 1993 1053 1063
52. M. Haupt, M.P. Blakeley, S.J. Fisher, S.A. Mason, J.B. Cooper, E.P. Mitchell, and et al. Binding site asymmetry in human transthyretin: Insights from a joint neutron and X-ray crystallographic analysis using perdeuterated protein IUCrJ Biology & Medicine 1 2014 429 438
53. M. Haupt, M.P. Blakeley, S.C.M. Teixeira, S.A. Mason, E.P. Mitchell, J.B. Cooper, and et al. Preliminary neutron crystallographic study of human transthyretin Acta Crystallographica F67 2011 1428 1431
54. I. Hazemann, M.-T. Dauvergne, M.P. Blakeley, F. Meilleur, M. Haertlein, A. Van Dorsselaer, and et al. High-resolution neutron protein crystallography with radically small crystal volumes: Application of perdeuteration to human aldose reductase Acta Crystallographica D 61 2005 1413 1417
55. E. Hellstrand, M. Grey, M.L. Ainalem, J. Ankner, V.T. Forsyth, G. Fragneto, and et al. Adsorption of α-synuclein to supported lipid bilayers: Positioning and role of electrostatics ACS Chemical Neuroscience 4 10 2013 1339 1351
56. Heumann, H. (2004). Method for labeling biopolymers using isotopes. US patent 6783751 B2.
57. 2O-containing minimal medium Journal of Biomolecular NMR 17 1 2000 33 42
58. D.W. Hoffman, and L.D. Spicer Isotopic labeling of specific amino acids as an aid to NMR spectrum assignment of the methionine repressor protein J.J. Villafranca, Techniques in protein chemistry: II 1991 Academic Press San Diego, CA 409 416
59. E.I. Howard, M.P. Blakeley, M. Haertlein, I. Petit-Haertlein, A. Mitschler, S.J. Fisher, and et al. Neutron structure of type-III antifreeze protein allows the reconstruction of AFP-ice interface Journal of Molecular Recognition 24 4 2011 724 732
60. B. Jacrot, C. Chauvin, and J. Witz Comparative neutron small-angle scattering study of small spherical RNA viruses Nature 266 5601 1977 417 421
61. M. Jasnin, M. Moulin, M. Haertlein, G. Zaccai, and M. Tehei In vivo measurement of internal and global macromolecular motions in E. coli Biophysical Journal 95 2 2008 857 864
62. M. Jasnin, M. Moulin, M. Haertlein, G. Zaccai, and M. Tehei Down to atomic scale intracellular water dynamics EMBO Reports 9 6 2008 545 547
63. M. Jasnin, M. Tehei, M. Moulin, M. Haertlein, and G. Zaccai Solvent isotope effect on macromolecular dynamics in E. coli European Biophysics Journal 37 5 2008 613 617
64. R. Jünemann, J. Wadzack, F.J. Triana-Alonso, J.U. Bittner, J. Caillet, T. Meinnel, and et al. In vivo deuteration of transfer RNAs: Overexpression and large-scale purification of deuterated specific tRNAs Nucleic Acids Research 24 5 1996 907 913
65. J.J. Katz, and H.L. Crespi Deuterated organisms: Cultivation and uses Science 151 1966 1187 1194
66. C.K. Kennaway, J.E. Taylor, C.F. Song, W. Potrzebowski, W. Nicholson, J.H. White, and et al. Structure and operation of the DNA-translocating Type I DNA restriction enzymes Genes & Development 26 2012 92D 104D
67. T. Kigawa, T. Yabukia, Y. Yoshida, M. Tsutsuic, Y. Itod, T. Shibatad, and et al. Cell-free production and stable-isotope labeling of milligram quantities of proteins FEBS Letters 442 1999 15 19
68. B.W. Koenig, M. Rogowski, and J.M. Louis A rapid method to attain isotope labeled small soluble peptides for NMR studies Journal of Biomolecular NMR 26 3 2003 193 202
69. A.Y. Kovalevsky, L. Hanson, S.Z. Fisher, M. Mustyakimov, S.A. Mason, V.T. Forsyth, and et al. Metal ion roles and the movement of hydrogen during reaction catalyzed by d-xylose isomerase: a joint X-ray and neutron diffraction study Structure 18 6 2010 688 699
70. A.Y. Kovalevsky, B.L. Hanson, S.A. Mason, T. Yoshida, S.Z. Fisher, M. Mustyakimov, and et al. Identification of the elusive hydronium ion exchanging roles with a proton in an enzyme at lower pH values Angewandte Chemie International Edition in English 50 33 2011 7520 7523
71. I. Kwon, P. Wang, and D.A. Tirrell Design of a bacterial host for site-specific incorporation of p-bromophenylalanine into recombinant proteins Journal of the American Chemical Society 128 36 2006 11778 11783
72. P. Langan, V.T. Forsyth, A. Mahendrasingam, W.J. Pigram, W. Fuller, and S.A. Mason A high-angle neutron fibre diffraction study of the hydration of the A conformation of the DNA double helix Journal of Biomolecular Structure and Dynamics 10 3 1992 489 503
73. S. Laulumaa, M.P. Blakeley, A. Raasakka, M. Moulin, M. Härtlein, and P. Petri Kursula Production, crystallization, and neutron diffraction of fully deuterated human myelin peripheral membrane protein P2 Acta Crystallographica F 71 2015 1391 1395
74. V. Laux, P. Callow, D.I. Svergun, P.A. Timmins, V.T. Forsyth, and M. Haertlein Selective deuteration of tryptophan and methionine residues in maltose binding protein: A model system for neutron scattering European Biophysics Journal 37 6 2008 815 822
75. K.M. Lee, E.J. Androphy, and J.D. Baleja A novel method for selective isotope labeling of bacterially expressed proteins Journal of Biomolecular NMR 5 1 1995 93 96
76. B. Leiting, F. Marsilio, and J.F. O'Connell Predictable deuteration of recombinant protein in Escherichia coli Analytical Biochemistry 265 1998 351 355
77. P.X. Li, R.K. Thomas, and J. Penfold Limitations in the use of surface tension and the Gibbs equation to determine surface excesses of cationic surfactants Langmuir 30 23 2014 6739 6747
78. W. Liu, E. Crocker, D.J. Siminovitch, and S.O. Smith Role of side-chain conformational entropy in transmembrane helix dimerization of glycophorin A Biophysical Journal 84 2 Pt. 1 2003 1263 1271
79. C.F. Ludlam, S. Sonar, C.P. Lee, M. Coleman, J. Herzfeld, U.L. RajBhandary, and et al. Site-directed isotope labeling and ATR-FTIR difference spectroscopy of bacteriorhodopsin: The peptide carbonyl group of Tyr 185 is structurally active during the bR→N transition Biochemistry 34 1 1995 2 6
80. A. Mahendrasingam, V.T. Forsyth, R. Hussain, R.J. Greenall, W.J. Pigram, and W. Fuller Time-resolved X-ray diffraction studies of the B to D structural transition in the DNA double helix Science 233 1986 195 197
81. S. Maric, N. Skar-Gislinge, S. Midtgaard, M.B. Thygesen, J. Schiller, H. Frielinghaus, and et al. Stealth carriers for low-resolution structure determination of membrane proteins in solution Acta Crystallographica D 70 Pt. 2 2014 317 328
82. S. Maric, M.B. Thygesen, J. Schiller, M. Marek, M. Moulin, M. Haertlein, and et al. Biosynthetic preparation of selectively deuterated phosphatidylcholine in genetically modified Escherichia coli Applied Microbiology and Biotechnology 99 1 2015 241 254
83. H. Masuda, M.S.R.C. Murthy, K. Suto, Y. Takenaka, P.K.R. Kumar, and H. Mizuno Expression and purification of deuterated proteins for neutron crystallography, Chapter 1, 47-60 N. Nimura, H. Mizuno, J.R. Helliwell, E. Westhof, Hydrogen- and hydration-sensitive structural biology Proceedings of Symposium on Development of New Structural Biology including Hydrogen and Hydration, Mito, Japan (2000) 2005
84. R.P. May, V. Nowotny, P. Nowotny, H. Voss, and K.H. Nierhaus Inter-protein distances within the large subunit from Escherichia coli ribosomes EMBO Journal 11 1991 373 378
85. F. Meilleur, M.T. Dauvergne, I. Schlichting, and D. Myles Production and X-ray crystallographic analysis of fully deuterated cytochrome P450cam Acta Crystallographica. Section D, Biological Crystallography 61 Pt. 5 2005 539 544
86. H.H. Niemann, M.V. Petoukhov, M. Härtlein, M. Moulin, E. Gherardi, P. Timmins, and et al. X-ray and neutron small-angle scattering analysis of the complex formed by the met receptor and the Listeria monocytogenes invasion protein InlB Journal of Molecular Biology 377 2 2008 489 500
87. Y. Nishiyama, P. Langan, M. Wada, and V.T. Forsyth Looking at hydrogen bonds in cellulose Acta Crystallographica D 66 2010 1172 1177
88. A. Paciaroni, E. Cornicchi, M. Marconi, A. Orecchini, C. Petrillo, M. Haertlein, and et al. Coupled relaxations at the protein-water interface in the picosecond timescale Journal of the Royal Society, Interface 6 2009 S635 S640
89. A. Paciaroni, A. Orecchini, E. Cornicchi, M. Marconi, C. Petrillo, M. Haertlein, and et al. Dynamical signature of protein hydration water reveals two populations of amorphous ice like behavior Physical Review Letters 101 14 2008 148104
90. A. Paciaroni, A. Orecchini, M. Haertlein, M. Moulin, V. Conti Nibali, A. De Francesco, and et al. Vibrational collective dynamics of dry proteins in the terahertz region The Journal of Physical Chemistry B 116 12 2012 3861 3865
91. D. Paliy, D.B. Bloor, P. Gilbert, and J. Barber Improved methods of cultivation and production of deuteriated proteins from E. coli strains grown on fully deuteriated minimal medium Journal of Applied Microbiology 94 4 2003 580 586
92. H. Patzelt, A.S. Ulrich, H. Egbringhoff, P. Düx, Ashurst J., B. Simon, and et al. Towards structural investigations on isotope labelled native bacteriorhodopsin in detergent micelles by solution-state NMR spectroscopy Journal of Biomolecular NMR 10 1997 95 106
93. J. Penfold, R.K. Thomas, P. Li, J.T. Petkov, I. Tucker, A.R. Cox, and et al. Impact of the degree of ethoxylation of the ethoxylated polysorbate nonionic surfactant on the surface self-assembly of hydrophobin-ethoxylated polysorbate surfactant mixtures Langmuir 30 32 2014 9741 9751
94. J. Penfold, R.K. Thomas, P.X. Li, J.T. Petkov, I. Tucker, J.R.P. Webster, and et al. Adsorption at air-water and oil-water interfaces and self-assembly in aqueous solution of ethoxylated polysorbate nonionic surfactants Langmuir 31 10 2015 3003 3011
95. J. Penfold, R.K. Thomas, P. Li, H. Xu, I.M. Tucker, J.T. Petkov, and et al. Multivalent-counterion-induced surfactant multilayer formation at hydrophobic and hydrophilic solid-solution interfaces Langmuir 31 24 2015 6773 6781
96. S.J. Perkins Estimation of deuteration levels in whole cells and cellular proteins by 1H NMR spectroscopy and neutron scattering Biochemical Journal 199 1981 163 170
97. I. Petit-Haertlein, M.P. Blakeley, E. Howard, I. Hazemann, A. Mitschler, M. Haertlein, and et al. Perdeuteration, purification, crystallization and preliminary neutron diffraction of an ocean pout type III antifreeze protein Acta Crystallographica F 65 4 2009 406 409
98. I. Petit-Haertlein, M.P. Blakeley, E. Howard, I. Hazemann, A. Mitschler, A. Podjarny, and et al. Incorporation of methyl-protonated valine and leucine residues into deuterated ocean pout Type III anti-freeze protein: Expression, crystallization and preliminary neutron diffraction studies Acta Crystallographica F 66 6 2010 665 669
99. S. Rajesh, D. Nietlispach, H. Nakayama, K. Takio, E.D. Laue, T. Shibata, and et al. A novel method for the biosynthesis of deuterated proteins with selective protonation at the aromatic rings of Phe, Tyr and Trp Biomolecular NMR 27 1 2003 81 86
100. V. Ramesh, R.O. Frederick, S.E. Syed, C.F. Gibson, J.C. Yang, and G.C. Roberts The interactions of Escherichia coli trp repressor with tryptophan and with an operator oligonucleotide European Journal of Biochemistry 225 1994 601 608
101. 2H labeling and neutron scattering Proceedings of the National Academy of Sciences of the United States of America 95 9 1998 4970 4975
102. M.A. Refaei, A. Combs, D.J. Kojetin, J. Cavanagh, C. Caperelli, M. Rance, and et al. Observing selected domains in multi-domain proteins via sortase-mediated ligation and NMR spectroscopy Journal of Biomolecular NMR 49 1 2011 3 7
103. N. Rochel, F. Ciesielski, J. Godet, E. Moman, M. Roessle, C. Peluso-Iltis, and et al. Common architecture of nuclear receptor heterodimers on DNA direct repeat elements with different spacings Nature Structural and Molecular Biology 18 5 2011 564 570
104. M.I. Savenkova, J.D. Satterlee, J.E. Erman, W.F. Siems, and G.L. Helms Expression, purification, characterization, and NMR studies of highly deuterated recombinant cytochrome c peroxidase Biochemistry 40 40 2001 12123 12131
105. D. Sawada, Y. Nishiyama, P. Langan, V.T. Forsyth, S. Kimura, and M. Wada Water in crystalline fibers of dihydrate beta-chitin results in unexpected absence of intramolecular hydrogen bonding PLoS One 7 6 2012 e39376
106. D. Sawada, Y. Nishiyama, L. Petridis, R. Parthasarathi, S. Gnanakaran, V.T. Forsyth, and et al. Structure and dynamics of a complex of cellulose with EDA: Insights into the action of amines on cellulose Cellulose 20 2013 1563 1571
107. G. Schirò, Y. Fichou, F.-X. Gallat, K. Wood, F. Gabel, M. Moulin, and et al. Translational diffusion of hydration water correlates with functional motions in folded and intrinsically disordered proteins Nature Communications 16 6 2015 6490
108. E. Schneck, A. Schollier, A. Halperin, M. Moulin, M. Härtlein, M. Sferrazza, and et al. Neutron reflectometry elucidates density profiles of deuterated proteins adsorbed onto surfaces displaying poly(ethylene glycol) brushes: Evidence for primary adsorption Langmuir 29 46 2013 14178 14187
109. A. Seyedarabi, T.-T. To, S. Ali, S. Hussain, M. Fries, R. Madsen, and et al. Structural insights into substrate specificity and the anti beta-elimination mechanism of pectate lyase Biochemistry 49 3 2010 539 546
110. M.W. Shotton, L.H. Pope, V.T. Forsyth, R.C. Denny, J. Archer, P. Langan, and et al. New developments in instrumentation for fibre diffraction experiments Journal of Applied Crystallography 31 56 1998 758 776
111. M.W. Shotton, L.H. Pope, V.T. Forsyth, P. Langan, R.C. Denny, U. Giesen, and et al. A high-angle neutron fibre diffraction study of the hydration of deuterated A-DNA Biophysical Chemistry 69 1 1997 85 96
112. F. Shu, V. Ramakrishnan, and B.P. Schoenborn Enhanced visibility of hydrogen atoms by neutron crystallography on fully deuterated myoglobin Proceedings of the National Academy of Sciences of the United States of America 97 8 2000 3872 3877
113. N. Skar-Gislinge, and L. Arleth Small-angle scattering from phospholipid nanodiscs: Derivation and refinement of a molecular constrained analytical model form factor Physical Chemistry Chemical Physics 13 8 2011 3161 3170
114. L. Skrisovska, M. Schubert, and F.H. Allain Recent advances in segmental isotope labeling of proteins: NMR applications to large proteins and glycoproteins Journal of Biomolecular NMR 46 1 2009 51 65
115. S. Sobhanifar, S. Reckel, F. Junge, D. Schwarz, L. Kai, M. Karbyshev, and et al. Cell-free expression and stable isotope labelling strategies for membrane proteins Journal of Biomolecular NMR 46 1 2010 33 43
116. H.K. Sreenath, C.A. Bingman, B.W. Buchan, K.D. Seder, B.T. Burns, H.V. Geetha, and et al. Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium Protein Expression and Purification 40 2 2005 256 267
117. A. Strauss, F. Bitsch, B. Cutting, G. Fendrich, P. Graff, J. Liebetanz, and et al. Amino-acid-type selective isotope labeling of proteins expressed in Baculovirus-infected insect cells useful for NMR studies Journal of Biomolecular NMR 26 4 2003 367 372
118. M.P. Strub, F. Hoh, J.F. Sanchez, J.M. Strub, A. Bock, A. Aumelas, and et al. Selenomethionine and selenocysteine double labeling strategy for crystallographic phasing Structure 11 11 2003 1359 1367
119. F.W. Studier, A.H. Rosenberg, J.J. Dunn, and J.W. Dubendorf Use of T7 RNA polymerase to direct expression of cloned genes Methods in Enzymology 185 1990 60 89
120. J.M. Studts, and B.G. Fox Application of fed-batch fermentation to the preparation of isotopically labelled or selenomethionyl-labelled proteins Protein Expression and Purification 16 1 1999 109 119
121. NT PLoS One 7 4 2012 e35263
122. S. Teixeira, J.F. Ankner, M.C. Bellissant-Funel, R. Bewley, M.P. Blakeley, L. Coates, and et al. New sources and instruments for neutrons in biology Chemical Physics 345 2-3 2008 133 151
123. L. Thomas, V.T. Forsyth, A. Sturcova, C.J. Kennedy, R.P. May, C.M. Altaner, and et al. Structure of cellulose microfibrils in primary cell-walls from collenchymas Plant Physiology 161 2013 465 476
124. 2O isotopic replacement Acta Crystallographica F 67 3 2011 332 335
125. V.U. Tuominen, D. Myles, M.-T. Dauvergne, R. Lahti, P. Heikinheimo, and A. Goldman Production and preliminary analysis of perdeuterated yeast inorganic pyrophosphatase crystals suitable for neutron diffraction Acta Crystallographica D60 3 2004 606 609
126. K. Vanatalu, T. Paalme, R. Vilu, N. Burkhardt, R. Junemann, R. May, and et al. Large-scale preparation of fully deuterated cell components: Ribosomes from Escherichia coli with high biological activity European Journal of Biochemistry 216 1 1993 315 321
127. 13C solid state NMR spectra of a 7-TM protein Biochimica et Biophysica Acta 1768 12 2007 3029 3035
128. 15N labeling of proteins for NMR studies Journal of Biomolecular NMR 5 4 1995 339 344
129. S. Vijayakrishnan, P. Callow, M.A. Nutley, D. McGow, D. Gilbert, P. Kropholler, and et al. Mammalian pyruvate dehydrogenase complex E2/E3BP core organization: A new variable composition model Biochemical Journal 437 2011 565 574
130. S. Vijayakrishnan, S.M. Kelly, R.J.C. Gilbert, P. Callow, D. Bhella, V.T. Forsyth, and et al. Solution structure and characterisation of the human pyruvate dehydrogenase complex core assembly Journal of Molecular Biology 399 2010 71 93
131. 15N-amino acids Journal of Biomolecular NMR 8 1996 184 192
132. J. White, D. Heß, J. Raynes, V. Laux, M. Haertlein, V.T. Forsyth, and et al. The aggregation of native human serum albumin European Biophysics Journal 44 5 2015 367 371
133. K. Wood, A. Froelich, A. Paciaroni, M. Moulin, M. Härtlein, G. Zaccai, and et al. Coincidence of dynamical transitions in a soluble protein and its hydration water: Direct measurements by neutron scattering and MD simulations Journal of the American Chemical Society 130 14 2008 4586 4587
134. K. Wood, F.X. Gallat, R. Otten, A.J. van Heel, M. Lethier, L. van Eijck, and et al. Protein surface and core dynamics show concerted hydration-dependent activation Angewandte Chemie 52 2 2013 665 668
135. K. Wood, S. Grudinin, B. Kessler, M. Weik, M. Johnson, G.R. Kneller, and et al. Dynamical heterogeneity of specific amino acids in bacteriorhodopsin Journal of Molecular Biology 380 3 2008 581 591
136. Z. Wu, V. Gogonea, X. Lee, M.A. Wagner, X.M. Li, Y. Huang, and et al. The double super helix model of high density lipoprotein The Journal of Biological Chemistry 284 52 2009 36605 36619
137. T. Yabuki, T. Kigawa, N. Dohmae, K. Takio, T. Terada, Y. Ito, and et al. Dual amino acid-selective and site-directed stable-isotope labeling of the human c-Ha-Ras protein by cell-free synthesis Journal of Biomolecular NMR 11 3 1998 295 306
138. G. Zaccai Neutron scattering experiments for protein dynamics Journal of Non-Crystalline Solids 357 2011 615 621
139. X. Zhu, S.Y. Ng, A.N. Gupta, Y.P. Feng, B. Ho, A. Lapp, and et al. Effect of crowding on the conformation of interwound DNA strands from neutron scattering measurements and Monte Carlo simulations Physical Review E 81 2010 061905