Cell-free protein synthesis of perdeuterated proteins for NMR studies

Journal of Biomolecular NMR

Volumn 39, Issue 3, 2007, Pages 229-238

  Etezady Esfarjani, Touraj ^a   Hiller, Sebastian ^a   Villalba, Cristina ^a   Wüthrich, Kurt ^a,b  

^a ETH ZURICH   (Switzerland)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Back protonation of deuterated amino acids; Cell free protein synthesis; Deuterated proteins; GroEL; Isotope labeling

Indexed keywords

AMINO ACID; BACTERIAL RNA; DEUTERIUM; FK 506 BINDING PROTEIN; HYDROGEN; LIGAND; RNA POLYMERASE;

ARTICLE; CELL FREE SYSTEM; CONTROLLED STUDY; DRUG RESEARCH; ENZYME ACTIVE SITE; ISOTOPE LABELING; MOLECULAR WEIGHT; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; OLIGOMERIZATION; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GROEL PROTEIN; ISOTOPE LABELING; MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN BIOSYNTHESIS; TACROLIMUS BINDING PROTEINS;

EID: 35448953299     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-007-9188-0     Document Type: Article

References (31)

1. Adrain C, Duriez PJ, Brumatti G, Delivani P, Martin SJ (2006) The cytotoxic lymphocyte protease, granzyme B, targets the cytoskeleton and perturbs microtubule polymerization dynamics. J Biol Chem 281:8118-8125
2. Bartels C., Güntert P., Wüthrich K. (1995) IFLAT-A new automatic baseline-correction method for multidimensional NMR spectra with strong solvent signals J Magn Reson A 117:330-333
3. De Marco A, Wüthrich K (1976) Digital filtering with a sinusoidal window function: An alternative technique for resolution enhancement in FT NMR. J Magn Reson 24:201-204
4. Ezure T, Suzuki T, Higashide S, Shintani E, Endo K, Kobayashi S, Shikata M, Ito M, Tanimizu K, Nishimura O (2006) Cell-free protein synthesis system prepared from insect cells by freeze-thawing. Biotechnol Prog 22:1570-1577
5. Fiaux J, Bertelsen EB, Horwich AL, Wüthrich K (2002) NMR analysis of a 900K GroEL GroES complex. Nature 418:207-211
6. Güntert P, Dötsch V, Wider G, Wüthrich K (1992) Processing of multi-dimensional NMR data with the new software PROSA. J Biomol NMR 2:619-629
7. Kainosho M, Torizawa T, Iwashita Y, Terauchi T, Mei Ono A, Güntert P (2006) Optimal isotope labelling for NMR protein structure determinations. Nature 440:52-57
8. Katzen F, Kudlicki W (2006) Efficient generation of insect-based cell-free translation extracts active in glycosylation and signal sequence processing. J Biotechnol 125:194-197
9. Kigawa T, Muto Y, Yokoyama S (1995) Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis. J Biomol NMR 6:129-134
10. Kigawa T, Yabuki T, Matsuda N, Matsuda T, Nakajima R, Tanaka A, Yokoyama S (2004) Preparation of Escherichia coli cell extract for highly productive cell-free protein expression. J Struct Funct Genomics 5:63-68
11. Klammt C, Löhr F, Schäfer B, Haase W, Dötsch V, Rüterjans H, Glaubitz C, Bernhard F (2004) High level cell-free expression and specific labeling of integral membrane proteins. Eur J Biochem 271:568-580
12. LeMaster DM (1989) Deuteration in protein proton magnetic resonance. Methods Enzymol 177:23-43
13. Mikami S, Kobayashi T, Yokoyama S, Imataka H (2006) A hybridoma-based in vitro translation system that efficiently synthesizes glycoproteins. J Biotechnol 127:65-78
14. Ozawa K, Headlam MJ, Schaeffer PM, Henderson BR, Dixon NE, Otting G (2004) Optimization of an Escherichia coli system for cell-free synthesis of selectively 15 N-labelled proteins for rapid analysis by NMR spectroscopy. Eur J Biochem 271:4084-4093
15. Pachter R, Arrowsmith CH, Jardetzky O (1992) The effect of selective deuteration on magnetization transfer in larger proteins. J Biomol NMR 2:183-194
16. Riek R, Fiaux J, Bertelsen EB, Horwich AL, Wüthrich K (2002) Solution NMR techniques for large molecular and supramolecular structures. J Am Chem Soc 124:12144-12153
17. Rosen MK, Michnick SW, Karplus M, Schreiber SL (1991) Proton and nitrogen sequential assignments and secondary structure determination of the human FK506 and rapamycin binding protein. Biochemistry 30:4774-4789
18. Sakurai N, Moriya K, Suzuki T, Sofuku K, Mochiki H, Nishimura O, Utsumi T (2006) Detection of co- and posttranslational protein N-myristoylation by metabolic labeling in an insect cell-free protein synthesis system. Anal Biochem 362:236-244
19. Schaffitzel C, Ban N (2007) Generation of ribosome nascent chain complexes for structural and functional studies. J Struct Biol 159:302-310
20. Sengupta S, Shah M, Nagaraja V (2006) Glutamate racemase from Mycobacterium tuberculosis inhibits DNA gyrase by affecting its DNA-binding. Nucleic Acids Res 34:5567-5576
21. Shimizu Y, Kanamori T, Ueda T (2005) Protein synthesis by pure translation systems. Methods 36:299-304
22. Spirin AS, Baranov VI, Ryabova LA, Ovodov SY, Alakhov YB (1988) A continuous cell-free translation system capable of producing polypeptides in high yield. Science 242:1162-1164
23. 15N backbone assignment of a 32 kDa hypothetical protein from Arabidopsis thaliana, At3g16450.1. J Biomol NMR 30:357-358
24. Torizawa T, Shimizu M, Taoka M, Miyano H, Kainosho M (2004) Efficient production of isotopically labeled proteins by cell-free synthesis: A practical protocol. J Biomol NMR 30:311-325
25. Veiga P, Piquet S, Maisons A, Furlan S, Courtin P, Chapot-Chartier MP, Kulakauskas S (2006) Identification of an essential gene responsible for D-Asp incorporation in the Lactococcus lactis peptidoglycan crossbridge. Mol Microbiol 62:1713-1724
26. Vinarov DA, Lytle BL, Peterson FC, Tyler EM, Volkman BF, Markley JL (2004) Cell-free protein production and labeling protocol for NMR-based structural proteomics. Nature Methods 1:1-5
27. Wakiyama M, Kaitsu Y, Yokoyama S (2006) Cell-free translation system from Drosophila S2 cells that recapitulates RNAi. Biochem Biophys Res Commun 343:1067-1071
28. 15N-amino acids. J Biomol NMR 8:184-192
29. Wüthrich K (1986) NMR of proteins and nucleic acids, Wiley, New York, NY
30. 15N assignments and secondary structure of the FK506 binding protein when bound to ascomycin. Biopolymers 33:535-550
31. Xu Z, Horwich AL, Sigler PB (1997) The crystal structure of the asymmetric GroEL-GroES-(ADP)7 chaperonin complex. Nature 388:741-750