Biosynthetic preparation of selectively deuterated phosphatidylcholine in genetically modified Escherichia coli

Applied Microbiology and Biotechnology

Volumn 99, Issue 1, 2015, Pages 241-254

  Maric, Selma ^a,b   Thygesen, Mikkel B ^b   Schiller, Jürgen ^c   Marek, Magdalena ^b   Moulin, Martine ^d,e   Haertlein, Michael ^d   Forsyth, V Trevor ^d,e   Bogdanov, Mikhail ^f   Dowhan, William ^f   Arleth, Lise ^a   Pomorski, Thomas Günther ^b  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c UNIVERSITY OF LEIPZIG   (Germany)

^d INSTITUT LAUE LANGEVIN   (France)

^e KEELE UNIVERSITY   (United Kingdom)

^f UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

Author keywords

Biosynthesis; E. coli; Evolution in the test tube; Glycerophospholipids; Mass spectrometry; Neutron scattering; NMR; Phosphatidylcholine; Selective deuteration

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; CELL MEMBRANES; CYTOLOGY; ESCHERICHIA COLI; MASS SPECTROMETRY; MICELLES; NEUTRON SCATTERING; NUCLEAR MAGNETIC RESONANCE; PHOSPHOLIPIDS; PROTEINS;

DEUTERATIONS; E. COLI; GLYCEROPHOSPHOLIPIDS; PHOSPHATIDYLCHOLINE; TEST TUBE;

DEUTERIUM;

CARBON; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLSERINE SYNTHASE; CULTURE MEDIUM; DEUTERIUM;

ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIAL STRAIN; BIOSYNTHESIS; CARBON SOURCE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; ESCHERICHIA COLI; LIPID ANALYSIS; LIPID COMPOSITION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PROTON NUCLEAR MAGNETIC RESONANCE; TRANSGENIC ORGANISM; WILD TYPE; CHEMISTRY; CULTURE MEDIUM; GENETICS; METABOLIC ENGINEERING; METABOLISM; PROCEDURES; STAINING;

CULTURE MEDIA; DEUTERIUM; ESCHERICHIA COLI; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; PHOSPHATIDYLCHOLINES; STAINING AND LABELING;

EID: 84925535388     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-6082-z     Document Type: Article

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