Observing selected domains in multi-domain proteins via sortase-mediated ligation and NMR spectroscopy

Journal of Biomolecular NMR

Volumn 49, Issue 1, 2011, Pages 3-7

  Refaei, Mary Anne ^a,d   Combs, Al ^b   Kojetin, Douglas J ^b,e   Cavanagh, John ^c   Caperelli, Carol ^a   Rance, Mark ^b   Sapitro, Jennifer ^a   Tsang, Pearl ^a  

^a UNIVERSITY OF CINCINNATI   (United States)

^b UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

^c NORTH CAROLINA STATE UNIVERSITY   (United States)

^d OHIO STATE UNIVERSITY   (United States)

^e SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Enzyme mediated ligation; MecA; NMR; Protein ligation; Segmental labeling; Sortase A

Indexed keywords

SORTASE;

ARTICLE; BACILLUS SUBTILIS; CHEMICAL LABELING; HETERONUCLEAR SINGLE QUANTUM COHERENCE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEAR OVERHAUSER EFFECT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN STRUCTURE; STAPHYLOCOCCUS AUREUS;

AMINO ACID SEQUENCE; AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; CYSTEINE ENDOPEPTIDASES; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN STRUCTURE, TERTIARY; PROTEINS; RECOMBINANT FUSION PROTEINS; STAPHYLOCOCCUS AUREUS;

STAPHYLOCOCCUS AUREUS;

EID: 79951556764     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-010-9464-2     Document Type: Article

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