Dual amino acid-selective and site-directed stable-isotope labeling of the human c-Ha-Ras protein by cell-free synthesis

Journal of Biomolecular NMR

Volumn 11, Issue 3, 1998, Pages 295-306

  Yabuki, Takashi ^a,b   Kigawa, Takanori ^a   Dohmae, Naoshi ^a   Takio, Koji ^a   Terada, Tohru ^a,b   Ito, Yutaka ^a   Laue, Ernest D ^c   Cooper, Jonathan A ^d   Kainosho, Masatsune ^e   Yokoyama, Shigeyuki ^a,b  

^a INST OF PHYS AND CHEMICAL RESEARCH   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^d Fred Hutchinson Cancer Research Center   (United States)

^e TOKYO METROPOLITAN UNIVERSITY   (Japan)

Author keywords

Cell free protein synthesis; Selective labeling; Site directed labeling; Stable isotope

Indexed keywords

ESCHERICHIA COLI;

AMINO ACID; ASPARTIC ACID; CARBON; NITROGEN; ONCOPROTEIN; PROLINE; RAS PROTEIN; SERINE; THREONINE; TYROSINE;

ARTICLE; BINDING SITE; BIOSYNTHESIS; CELL FREE SYSTEM; CHEMICAL MODEL; HUMAN; ISOTOPE LABELING; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; SITE DIRECTED MUTAGENESIS;

AMINO ACIDS; ASPARTIC ACID; BINDING SITES; CARBON ISOTOPES; CARBON RADIOISOTOPES; CELL-FREE SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; ISOTOPE LABELING; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; ONCOGENE PROTEINS V-RAF; PROLINE; RAS PROTEINS; RETROVIRIDAE PROTEINS, ONCOGENIC; SERINE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; THREONINE; TYROSINE;

EID: 0032036838     PISSN: 09252738     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008276001545     Document Type: Article

References (43)

1. Akashi, S., Shirouzu, M., Yokoyama, S. and Takio, K. (1996) J. Mass Spectrom. Soc. Jpn., 44, 269-277.
2. Arata, Y., Kato, K., Takahashi, H. and Shimada, I. (1994) Methods Enzymol., 239, 440-464.
3. Bain, J.D., Diala, E.S., Glabe, C.G., Wacker, D.A., Lyttle, M.H., Dix, T.A. and Chamberlin, A.R. (1991) Biochemistry, 30, 5411-5421.
4. Bodenhausen, G. and Ruben, D.J. (1980) Chem. Phys. Lett., 69, 185-199.
5. Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J. and Bax, A. (1995) J. Biomol. NMR, 6, 277-293.
6. Ellman, J.A., Mendel, D., Anthony-Cahill, S., Noren, C.J. and Schultz, P.G. (1991) Methods Enzymol., 202, 301-336.
7. Ellman, J.A., Volkman, B.F., Mendel, D., Schultz, P.G. and Wemmer, D.E. (1992) J. Am. Chem. Soc., 114, 7959-7961.
8. Grzesiek, S. and Bax, A. (1993) J. Am. Chem. Soc., 115, 12593-12594.
9. Ha, J.-M., Ito, Y., Kawai, G., Miyazawa, T., Miura, K., Ohtsuka, E., Noguchi, S., Nishimura, S. and Yokoyama, S. (1989) Biochemistry, 28, 8411-8416.
10. Ikura, M., Kay, L.E. and Bax, A. (1990) Biochemistry, 29, 4659-4667.
11. Ito, Y., Yamasaki, K., Iwahara, J., Terada, T., Kamiya, A., Shirouzu, M., Muto, Y., Kawai, G., Yokoyama, S., Laue, E.D., Wälchli, M., Shibata, T., Nishimura, S. and Miyazawa, T. (1997) Biochemistry, 36, 9109-9119.
12. Johnson, B.A. and Blevins, R.A. (1994) J. Biomol. NMR, 4, 603-614.
13. Kainosho, M., Nagao, H. and Tsuji, T. (1987) Biochemistry, 26, 1068-1075.
14. Kainosho, M., Nagao, H., Imamura, Y., Uchida, K., Tomonaga, N., Nakamura, Y. and Tsuji, T. (1985) J. Mol. Struct., 126, 549-562.
15. Kainosho, M. and Tsuji, T. (1982) Biochemistry, 21, 6273-6279.
16. Kato, K., Matsunaga, C., Nishimura, Y., Wälchli, M., Kainosho, M. and Arata, Y. (1989) J. Biochem. (Tokyo), 105, 867-869.
17. Ketchem, R.R., Lee, K.C., Huo, S. and Cross, T.A. (1996) J. Biomol. NMR, 8, 1-14.
18. Kigawa, T., Muto, Y. and Yokoyama, S. (1995) J. Biomol. NMR, 6, 129-134.
19. Kim, D.-M., Kigawa, T., Choi, C.-Y. and Yokoyama, S. (1996) Eur. J. Biochem., 239, 881-886.
20. Kleina, L.G., Masson, J.M., Normanly, J., Abelson, J. and Miller, J.H. (1990) J. Mol. Biol., 213, 705-717.
21. Liu, D.R., Magliery, T.J., Pasternak, M. and Schultz, P.G. (1997) Proc. Natl. Acad. Sci. USA, 94, 10092-10097.
22. Liu, X.M., Sonar, S., Lee, C.P., Coleman, M., RajBhandary, U.L. and Rothschild, K.J. (1995) Biophys. Chem., 56, 63-70.
23. Lodder, M., Golovine, S. and Hecht, S.M. (1997) J. Org. Chem., 62, 778-779.
24. McIntosh, L.P. and Dahlquist, F.W. (1990) Q. Rev. Biophys., 23, 1-38.
25. Milburn, M.V., Tong, L., de Vos, A.M., Brunger, A., Yamaizumi, Z., Nishimura, S. and Kim, S.H. (1990) Science, 247, 939-945.
26. Muto, Y., Yamasaki, K., Ito, Y., Yajima, S., Masaki, H., Uozumi, T., Wälchli, M., Nishimura, S., Miyazawa, T. and Yokoyama, S. (1993) J. Biomol. NMR, 3, 165-184.
27. Nassar, N., Horn, G., Herrmann, C., Block, C., Janknecht, R. and Wittinghofer, A. (1996) Nat. Struct. Biol., 3, 723-729.
28. Nassar, N., Horn, G., Herrmann, C., Scherer, A., McCormick, F. and Wittinghofer, A. (1995) Nature, 375, 554-560.
29. Noren, C.J., Anthony-Cahill, S.J., Griffith, M.C. and Schultz, P.G. (1989) Science, 244, 182-188.
30. Normanly, J., Kleina, L.G., Masson, J.M., Abelson, J. and Miller, J.H. (1990) J. Mol. Biol., 213, 719-726.
31. Pai, E.F., Kabsch, W., Krengel, U., Holmes, K.C., John, J. and Wittinghofer, A. (1989) Nature, 341, 209-214.
32. Peersen, O.B., Yoshimura, S., Hojo, H., Aimoto, S. and Smith, S.O. (1992) J. Am. Chem. Soc., 114, 4332-4335.
33. Pratt, J.M. (1984) In Transcription and Translation (Eds, Hames, B.D. and Higgins, S.J.), IRL Press, Oxford, pp. 179-209.
34. Robertson, S.A., Ellman, J.A. and Schultz, P.G. (1991) J. Am. Chem. Soc., 113, 2722-2729.
35. Schagger, H. and von Jagow, G. (1987) Anal. Biochem., 166, 368-379.
36. Schmitt, E., Meinnel, T., Panvert, M., Mechulam, Y. and Blanquet, S. (1993) J. Mol. Biol., 233, 615-628.
37. Shulman, L.H. (1991) Prog. Nucleic Acid Res. Mol. Biol., 41, 23-87.
38. Sonar, S., Lee, C.-P., Coleman, M., Patel, N., Liu, X., Marti, T., Khorana, H.G., RajBhandary, U.L. and Rothschild, K.J. (1994) Nat. Struct. Biol., 1, 512-517.
39. Studier, F.W., Rosenberg, A.H., Dunn, J.J. and Dubendorff, J.W. (1990) Methods Enzymol., 185, 60-89.
40. Westler, W.M., Kainosho, M., Nagao, H., Tomonaga, N. and Markley, J.L. (1988a) J. Am. Chem. Soc., 110, 4093-4095.
41. Westler, W.M., Stockman, B.J., Markley, J.L., Hosoya, Y., Miyake, Y. and Kainosho, M. (1988b) J. Am. Chem. Soc., 110, 6256-6258.
42. Yamasaki, K., Muto, Y., Ito, Y., Wälchli, M., Miyazawa, T., Nishimura, S. and Yokoyama, S. (1992) J. Biomol. NMR, 2, 71-82.
43. Zawadzki, V. and Gross, H.J. (1991) Nucleic Acids Res., 19, 1948.