Cytochrome P450 induction response in tethered spheroids as a three-dimensional human hepatocyte in vitro model

Journal of Applied Toxicology

Volumn 36, Issue 2, 2016, Pages 320-329

  Xia, Lei ^a   Hong, Xin ^a   Sakban, Rashidah Binte ^a,b   Qu, Yinghua ^b   Singh, Nisha Hari ^b   Mcmillian, Michael ^c   Dallas, Shannon ^c   Silva, Jose ^c   Sensenhauser, Carlo ^c   Zhao, Sylvia ^d   Lim, Heng Keang ^c   Yu, Hanry ^{a,b,e,f,g,h,i,j}  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b AGENCY FOR SCIENCE   (Singapore)

^c JANSSEN RESEARCH AND DEVELOPMENT   (United States)

^d JANSSEN RESEARCH AND DEVELOPMENT   (Belgium)

^e NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^f SINGAPORE MIT ALLIANCE   (Singapore)

^g NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^h NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

ⁱ SINGAPORE MIT ALLIANCE FOR RESEARCH AND TECHNOLOGY   (Singapore)

^j Massachusetts Institute of Technology   (United States)

more..

Author keywords

3D in vitro model; Cryopreserved human hepatocyte; Cytochrome P450; Galactose; RGD; Spheroid

Indexed keywords

ARGINYLGLYCYLASPARTIC ACID; COLLAGEN; CYTOCHROME P450 1A2; CYTOCHROME P450 2B6; CYTOCHROME P450 3A4; GALACTOSE; CYTOCHROME P450;

ADULT; ARTICLE; CELL ADHESION; CELL FUNCTION; CELL MIGRATION; CELL STRUCTURE; CELL VIABILITY; CHILD; CONTROLLED STUDY; CRYOPRESERVATION; ENZYME ACTIVITY; ENZYME INDUCTION; FEMALE; HUMAN; HUMAN CELL; IN VITRO STUDY; INFANT; LIVER CELL; LIVER CELL CULTURE; LIVER FUNCTION; MALE; PRESCHOOL CHILD; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRANSCRIPTION REGULATION; TUMOR SPHEROID; CELL CULTURE; DRUG EFFECTS; DRUG INTERACTION; DRUG SCREENING; METABOLISM; PATHOLOGY; PHYSIOLOGY; PROCEDURES; TOXIC HEPATITIS;

CELLS, CULTURED; CHEMICAL AND DRUG INDUCED LIVER INJURY; CYTOCHROME P-450 ENZYME SYSTEM; DRUG EVALUATION; DRUG INTERACTIONS; HEPATOCYTES; HUMANS;

EID: 84954374227     PISSN: 0260437X     EISSN: 10991263     Source Type: Journal    
DOI: 10.1002/jat.3189     Document Type: Article

References (40)

1. Alexandre E, Baze A, Parmentier C, Desbans C, Pekthong D, Gerin B, Wack C, Bachellier P, Heyd B, Weber JC, Richert L. 2012. Plateable cryopreserved human hepatocytes for the assessment of cytochrome P450 inducibility: experimental condition-related variables affecting their response to inducers. Xenobiotica 42: 968-979.
2. Bhandari RN, Riccalton LA, Lewis AL, Fry JR, Hammond AH, Tendler SJ, Shakesheff KM. 2001. Liver tissue engineering: a role for co-culture systems in modifying hepatocyte function and viability. Tissue Eng. 7: 345-357.
3. Brandon EF, Raap CD, Meijerman I, Beijnen JH, Schellens JH. 2003. An update on in vitro test methods in human hepatic drug biotransformation research: pros and cons. Toxicol. Appl. Pharmacol. 189: 233-246.
4. Brophy CM, Luebke-Wheeler JL, Amiot BP, Khan H, Remmel RP, Rinaldo P, Nyberg SL. 2009. Rat hepatocyte spheroids formed by rocked technique maintain differentiated hepatocyte gene expression and function. Hepatology 49: 578-586.
5. Chai X, Zeng S, Xie W. 2013. Nuclear receptors PXR and CAR: implications for drug metabolism regulation, pharmacogenomics and beyond. Expert Opin. Drug Metab. Toxicol. 9: 253-266.
6. Charras G, Paluch E. 2008. Blebs lead the way: how to migrate without lamellipodia. Nat. Rev. Mol. Cell Biol. 9: 730-736.
7. Chua KN, Lim WS, Zhang P, Lu H, Wen J, Ramakrishna S, Leong KW, Mao HQ. 2005. Stable immobilization of rat hepatocyte spheroids on galactosylated nanofiber scaffold. Biomaterials 26: 2537-2547.
8. Danielson PB. 2002. The cytochrome P450 superfamily: biochemistry, evolution and drug metabolism in humans. Curr. Drug Metab. 3: 561-597.
9. Du Y, Chia SM, Han R, Chang S, Tang H, Yu H. 2006. 3D hepatocyte monolayer on hybrid RGD/galactose substratum. Biomaterials 27: 5669-5680.
10. Dunn JC, Tompkins RG, Yarmush ML. 1991. Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration. Biotechnol. Prog. 7: 237-245.
11. Esser C. 2012. Biology and function of the aryl hydrocarbon receptor: report of an international and interdisciplinary conference. Arch. Toxicol. 86: 1323-1329.
12. Griffith LG, Swartz MA. 2006. Capturing complex 3D tissue physiology in vitro. Nat. Rev. Mol. Cell Biol. 7: 211-224.
13. Hamilton GA, Jolley SL, Gilbert D, Coon DJ, Barros S, LeCluyse EL. 2001. Regulation of cell morphology and cytochrome P450 expression in human hepatocytes by extracellular matrix and cell-cell interactions. Cell Tissue Res. 306: 85-99.
14. Huang W, Zhang J, Washington M, Liu J, Parant JM, Lozano G, Moore DD. 2005. Xenobiotic stress induces hepatomegaly and liver tumors via the nuclear receptor constitutive androstane receptor. Mol. Endocrinol. 19: 1646-1653.
15. Hynes RO. 2002. Integrins: bidirectional, allosteric signaling machines. Cell 110: 673-687.
16. Kim SH, Kim JH, Akaike T. 2003. Regulation of cell adhesion signaling by synthetic glycopolymer matrix in primary cultured hepatocyte. FEBS Lett. 553: 433-439.
17. Klieber S, Torreilles F, Guillou F, Fabre G. 2010. The use of human hepatocytes to investigate drug metabolism and CYP enzyme induction. Methods Mol Bio 640: 295-308.
18. Kocarek TA, Schuetz EG, Strom SC, Fisher RA, Guzelian PS. 1995. Comparative analysis of cytochrome P4503A induction in primary cultures of rat, rabbit, and human hepatocytes. Drug Metab. Dispos. 23: 415-421.
19. LeCluyse E, Bullock PAP. 1996. Strategies for restoration and maintenance of normal hepatic structure and function in long-term cultures of rat hepatocytes. Adv. Drug Deliv. Rev. 22: 133-186.
20. LeCluyse E, Madan A, Hamilton G, Carroll K, DeHaan R, Parkinson A. 2000. Expression and regulation of cytochrome P450 enzymes in primary cultures of human hepatocytes. J. Biochem. Mol. Toxicol. 14: 177-188.
21. Lessey EC, Guilluy C, Burridge K. 2012. From mechanical force to RhoA activation. Biochemistry 51: 7420-7432.
22. Li AP, Lu C, Brent JA, Pham C, Fackett A, Ruegg CE, Silber PM. 1999. Cryopreserved human hepatocytes: characterization of drug-metabolizing enzyme activities and applications in higher throughput screening assays for hepatotoxicity, metabolic stability, and drug-drug interaction potential. Chem. Biol. Interact. 121: 17-35.
23. Liebler DC, Guengerich FP. 2005. Elucidating mechanisms of drug-induced toxicity. Nat. Rev. Drug Discov. 4: 410-420.
24. Mooney D, Hansen L, Vacanti J, Langer R, Farmer S, Ingber D. 1992. Switching from differentiation to growth in hepatocytes: control by extracellular matrix. J. Cell. Physiol. 151: 497-505.
25. Nishimura M, Narimatsu S, Naito S. 2009. Evaluation of induction potency of new drug candidates on CYP1A2 and CYP3A4 using real-time one-step RT-PCR in primary cultures of cryopreserved human hepatocytes. Drug Metab. Pharmacokinet. 24: 446-450.
26. Oda H, Yoshida Y, Kawamura A, Kakinuma A. 2008. Cell shape, cell-cell contact, cell-extracellular matrix contact and cell polarity are all required for the maximum induction of CYP2B1 and CYP2B2 gene expression by phenobarbital in adult rat cultured hepatocytes. Biochem. Pharmacol. 75: 1209-1217.
27. Parkinson A, Mudra DR, Johnson C, Dwyer A, Carroll KM. 2004. The effects of gender, age, ethnicity, and liver cirrhosis on cytochrome P450 enzyme activity in human liver microsomes and inducibility in cultured human hepatocytes. Toxicol. Appl. Pharmacol. 199: 193-209.
28. Pascussi JM, Gerbal-Chaloin S, Fabre JM, Maurel P, Vilarem MJ. 2000. Dexamethasone enhances constitutive androstane receptor expression in human hepatocytes: consequences on cytochrome P450 gene regulation. Mol. Pharmacol. 58: 1441-1450.
29. Pinkse GG, Voorhoeve MP, Noteborn M, Terpstra OT, Bruijn JA, De Heer E. 2004. Hepatocyte survival depends on beta1-integrin-mediated attachment of hepatocytes to hepatic extracellular matrix. Liver Int. 24: 218-226.
30. Rodriguez-Antona C, Donato MT, Boobis A, Edwards RJ, Watts PS, Castell JV, Gomez-Lechon MJ. 2002. Cytochrome P450 expression in human hepatocytes and hepatoma cell lines: molecular mechanisms that determine lower expression in cultured cells. Xenobiotica 32: 505-520.
31. Roymans D, Van Looveren C, Leone A, Parker JB, McMillian M, Johnson MD, Koganti A, Gilissen R, Silber P, Mannens G, Meuldermans W. 2004. Determination of cytochrome P450 1A2 and cytochrome P450 3A4 induction in cryopreserved human hepatocytes. Biochem. Pharmacol. 67: 427-437.
32. Rushmore TH, Kong AN. 2002. Pharmacogenomics, regulation and signaling pathways of phase I and II drug metabolizing enzymes. Curr. Drug Metab. 3: 481-490.
33. Sakai Y, Yamagami S, Nakazawa K. 2010. Comparative analysis of gene expression in rat liver tissue and monolayer- and spheroid-cultured hepatocytes. Cells Tissues Organs 191: 281-288.
34. Shen C, Meng Q. 2012. Prediction of cytochrome 450 mediated drug-drug interactions by three-dimensional cultured hepatocytes. Mini Rev. Med. Chem. 12: 1028-1036.
35. Tirona RG, Lee W, Leake BF, Lan LB, Cline CB, Lamba V, Parviz F, Duncan SA, Inoue Y, Gonzalez FJ, Schuetz EG, Kim RB. 2003. The orphan nuclear receptor HNF4alpha determines PXR- and CAR-mediated xenobiotic induction of CYP3A4. Nat. Med. 9: 220-224.
36. Tuschl G, Mueller SO. 2006. Effects of cell culture conditions on primary rat hepatocytes-cell morphology and differential gene expression. Toxicology 218: 205-215.
37. Vinken M, Papeleu P, Snykers S, De Rop E, Henkens T, Chipman JK, Rogiers V, Vanhaecke T. 2006. Involvement of cell junctions in hepatocyte culture functionality. Crit. Rev. Toxicol. 36: 299-318.
38. Wells RG. 2008. The role of matrix stiffness in regulating cell behavior. Hepatology 47: 1394-1400.
39. Xia L, Sakban RB, Qu Y, Hong X, Zhang W, Nugraha B, Tong WH, Ananthanarayanan A, Zheng B, Chau IY, Jia R, McMillian M, Silva J, Dallas S, Yu H. 2012. Tethered spheroids as an in vitro hepatocyte model for drug safety screening. Biomaterials 33: 2165-2176.
40. Ying L, Yin C, Zhuo RX, Leong KW, Mao HQ, Kang ET, Neoh KG. 2003. Immobilization of galactose ligands on acrylic acid graft-copolymerized poly(ethylene terephthalate) film and its application to hepatocyte culture. Biomacromolecules 4: 157-165.