Evaluation of induction potency of new drug candidates on CYP1A2 and CYP3A4 using real-time one-step RT-PCR in primary cultures of cryopreserved human hepatocytes

Drug Metabolism and Pharmacokinetics

Volumn 24, Issue 5, 2009, Pages 446-450

  Nishimura, Masuhiro ^a   Narimatsu, Shizuo ^b   Naito, Shinsaku ^a  

^a OTSUKA PHARMACEUTICAL CO LTD   (Japan)

^b OKAYAMA UNIVERSITY   (Japan)

Author keywords

Cryopreserved human hepatocytes; CYP1A2; CYP3A4; mRNA induction; New drug candidates; Primary cultures

Indexed keywords

4 [(4 BROMO 2 CYANOPHENYL)CARBAMOYL]BENZYLPHOSPHONIC ACID DIETHYL ESTER; BETA NAPHTHOFLAVONE; CYTOCHROME P450 1A2; CYTOCHROME P450 3A4; DIMETHYL SULFOXIDE; IBROLIPIM DERIVATIVE; MESSENGER RNA; NEW DRUG; PRIMER RNA; RIFAMPICIN; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; CRYOPRESERVATION; DRUG POTENCY; DRUG SCREENING; ENZYME INDUCTION; GENE EXPRESSION; HUMAN; HUMAN CELL; LIVER CELL CULTURE; OLIGONUCLEOTIDE PROBE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 71449105258     PISSN: 13474367     EISSN: 18800920     Source Type: Journal    
DOI: 10.2133/dmpk.24.446     Document Type: Article

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