Expression and regulation of cytochrome P450 enzymes in primary cultures of human hepatocytes

Journal of Biochemical and Molecular Toxicology

Volumn 14, Issue 4, 2000, Pages 177-188

  LeCluyse, Edward ^a   Madan, Ajay ^c   Hamilton, Geraldine ^a   Carroll, Kathy ^c   DeHaan, Ryan ^b,c   Parkinson, Andrew ^b,c  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b UNIVERSITY OF KANSAS SCHOOL OF MEDICINE   (United States)

^c XenoTech LLC   (United States)

Author keywords

Cytochrome P450; Enzyme Induction; Human Hepatocytes; In Vitro Models

Indexed keywords

CYTOCHROME P450; SOLVENT;

ARTICLE; BIOSYNTHESIS; CELL CULTURE; CYTOLOGY; DRUG SCREENING; ENZYME INDUCTION; ENZYMOLOGY; EXTRACELLULAR MATRIX; HUMAN; KINETICS; LIVER; METABOLISM; SENSITIVITY AND SPECIFICITY;

CELLS, CULTURED; CYTOCHROME P-450 ENZYME SYSTEM; DRUG EVALUATION; ENZYME INDUCTION; EXTRACELLULAR MATRIX; HUMANS; KINETICS; LIVER; SENSITIVITY AND SPECIFICITY; SOLVENTS;

EID: 0033655573     PISSN: 10956670     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1099-0461(2000)14:4<177::AID-JBT1>3.0.CO;2-4     Document Type: Article

References (14)

1. Parkinson A. Biotransformation of xenobiotics. In: Klaassen CD, editor. Casarett and Doull's toxicology: the basic science of poisons. 5th ed. New York: McGraw Hill; 1996. p 113-186.
2. Parkinson A. An overview of current cytochrome P450 technology for assessing' the safety and efficacy of new materials. Toxicol Pathol 1996;24:45-57.
3. Grasso P, Sharratt M, Cohen AJ. Role of persistent, non-genotoxic tissue damage in rodent cancer and relevance to humans. Annu Rev Pharmacol Toxicol 1991;31:253-287.
4. Conney AH. Pharmacological implications of microsomal enzyme induction. Pharmacol Rev 1967;19:317-366.
5. Li AP, Jurima-Romet M. Applications of primary human hepatocytes in the evaluation of pharmacokinetic drug-drug interactions: evaluation of model drugs terfenadine and rifampin. Cell Biol Toxicol 1997;13(4-5):365-374.
6. Li AP, Lu C, Brent JA, Pham C, Fackett A, Ruegg CE, Silber PM. Cryopreserved human hepatocytes: characterization of drug-metabolizing enzyme activities and applications in higher throughput screening assays for hepatotoxicity, metabolic stability, and drug-drug interaction potential. Chem Biol Interact 1999;121(l):17-35.
7. Kocarek TA, Schuetz EG, Strom SC, Fisher RA, Guzelian PS. Comparative analysis of cytochrome P4503A induction in primary cultures of rat, rabbit, and human hepatocytes. Drug Metab Dispos 1995;23(3):415-421.
8. Curi-Pedrosa R, Daujat M, Pichard L, Ourlin JC, Clair P, Gervot L, Lesca P, Domergue J, Joyeux H, Fourtanier G, Maurel P. Omeprazole and lansoprazole are mixed inducers of CYP1A and CYP3A in human hepatocytes in primary culture. J Pharmacol Exp Ther 1994;269:384-392.
9. Diaz D, Fabre I, Daujat M, Saint Aubert B, Bories P, Michel H, Maurel P. Omeprazole is an aryl hydrocarbon-like inducer of human hepatic cytochrome P450. Gastroenterology 1990;99:737-747.
10. Ged C, Rouillon JM, Pichard L, Combalbert J, Bressot N, Bories P, Michel H, Beaune P, Maurel P. The increase in urinary excretion of 6β-hydroxycortisol as a marker of human hepatic cytochrome P450IIIA induction. Br J Clin Pharmacol 1989;28:373-387.
11. Morel F, Beaune PH, Ratanasavanh D, Flinois J-P, Yang CS, Guengerich FP, Guillouzo A. Expression of cytochrome P-450 enzymes in cultured human hepatocytes. Eur J Biochem 1990;191:437-444.
12. Pichard L, Fabre I, Fabre G, Domergue J, Saint-Aubert B, Mourad G, Maurel P. Cyclosporin A drug interactions: screening for inducers and inhibitors of cytochrome P-450 (cyclosporin A oxidase) in primary cultures of human hepatocytes and in liver microsomes. Drug Metab Dispos 1990;18:595-606.
13. Wrighton SA, Maurel P, Schuetz EG, Watkins PB, Young B, Guzelian PS. Identification of the cytochrome P-450 induced by macrolide antibiotics in rat liver as the glucocorticoid responsive cytochrome P-450p. Biochemistry 1985;24:2171-2178.
14. Li AP, Roque MA, Beck DJ, Kaminski DL. Isolation and culturing of hepatocytes from human livers. Journal of Tissue Culture Methods 1992;14:139-146.