Coherent Raman Scattering Microscopy in Biology and Medicine

Annual Review of Biomedical Engineering

Volumn 17, Issue , 2015, Pages 415-445

  Zhang, Chi ^a   Zhang, Delong ^a   Cheng, Ji Xin ^a  

^a PURDUE UNIVERSITY   (United States)

Author keywords

Biomedical imaging; Clinical setting; Multimodal imaging; Raman scattering; Vibrational spectroscopy

Indexed keywords

MEDICAL IMAGING; MOLECULAR BIOLOGY; RAMAN SCATTERING; SIGNAL TO NOISE RATIO; SPECTROSCOPY; VIBRATIONAL SPECTROSCOPY;

BIOMEDICAL IMAGING; CLINICAL SETTINGS; COHERENT RAMAN SCATTERING; COHERENT RAMAN SCATTERING MICROSCOPIES; MULTI-MODAL IMAGING; TECHNICAL DEVELOPMENT; VIBRATIONAL SIGNATURE; VISUALIZATION AND ANALYSIS;

COHERENT SCATTERING;

BIOLOGY; IMAGING; MICROSCOPY; SIGNAL NOISE RATIO; VELOCITY; ANIMAL; BIOMEDICAL ENGINEERING; DEVICES; DIAGNOSTIC IMAGING; HUMAN; LASER; MULTIMODAL IMAGING; PROCEDURES; RAMAN SPECTROMETRY; STATISTICS AND NUMERICAL DATA; VIBRATION;

ANIMALS; BIOMEDICAL ENGINEERING; DIAGNOSTIC IMAGING; HUMANS; LASERS; MICROSCOPY; MULTIMODAL IMAGING; SIGNAL-TO-NOISE RATIO; SPECTRUM ANALYSIS, RAMAN; VIBRATION;

EID: 84949495994     PISSN: 15239829     EISSN: 15454274     Source Type: Book Series    
DOI: 10.1146/annurev-bioeng-071114-040554     Document Type: Review

References (182)

1. Raman CV, Krishnan KS. 1928. A new type of secondary radiation. Nature 121:501-2
2. Hendra PJ, Stratton PM. 1969. Laser-Raman spectroscopy. Chem. Rev. 69:325-44
3. DelhayeM, Dhamelincourt P. 1975. Raman microprobe and microscope with laser excitation. J. Raman Spectrosc. 3:33-43
4. Abraham J, Etz E. 1979. Molecular microanalysis of pathological specimens in situ with a laser-Raman microprobe. Science 206:716-18
5. Palonpon AF, Ando J, Yamakoshi H, Dodo K, Sodeoka M, et al. 2013. Raman and SERS microscopy for molecular imaging of live cells. Nat. Protoc. 8:677-92
6. Yamakoshi H, Dodo K, Okada M, Ando J, Palonpon A, et al. 2011. Imaging of EdU, an alkyne-tagged cell proliferation probe, by Raman microscopy. J. Am. Chem. Soc. 133:6102-5
7. Cheng J-X, Xie XS. 2012. Coherent Raman Scattering Microscopy. London: CRC
8. Boyd RW. 2003. Nonlinear Optics. Burlington, MA: Academic. 2nd ed.
9. Cheng J-X, Xie XS. 2003. Coherent anti-Stokes Raman scattering microscopy: instrumentation, theory, and applications. J. Phys. Chem. B 108:827-40
10. Terhune RW, Maker PD, Savage CM. 1965. Measurements of nonlinear light scattering. Phys. Rev. Lett. 14:681-84
11. Régnier PR, Taran JPE. 1973. On the possibility of measuring gas concentrations by stimulated anti-Stokes scattering. Appl. Phys. Lett. 23:240-42
12. Gord JR,Meyer TR, Roy S. 2008. Applications of ultrafast lasers for optical measurements in combusting flows. Annu. Rev. Anal. Chem. 1:663-87
13. Duncan MD, Reintjes J, Manuccia TJ. 1982. Scanning coherent anti-Stokes Raman microscope. Opt. Lett. 7:350-52
14. Zumbusch A, Holtom GR,Xie XS. 1999. Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering. Phys. Rev. Lett. 82:4142-45
15. Cheng J-X, Book LD, Xie XS. 2001. Polarization coherent anti-Stokes Raman scattering microscopy. Opt. Lett. 26:1341-43
16. Cheng J-X, Jia YK, Zheng G, Xie XS. 2002. Laser-scanning coherent anti-Stokes Raman scattering microscopy and applications to cell biology. Biophys. J. 83:502-9
17. Volkmer A, Cheng J-X, Sunney Xie X. 2001. Vibrational imaging with high sensitivity via epidetected coherent anti-Stokes Raman scattering microscopy. Phys. Rev. Lett. 87:023901
18. Evans CL, Potma EO, Puoris'haag M, Coté D, Lin CP, Xie XS. 2005. Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy. PNAS 102:16807-12
19. Cheng J-X, Volkmer A, Xie XS. 2002. Theoretical and experimental characterization of coherent anti-Stokes Raman scattering microscopy. J. Opt. Soc. Am. B 19:1363-75
20. Cheng J-X, Volkmer A, Book LD, Xie XS. 2002. Multiplex coherent anti-Stokes Raman scattering microspectroscopy and study of lipid vesicles. J. Phys. Chem. B 106:8493-98
21. MüllerM, Schins JM. 2002. Imaging the thermodynamic state of lipid membranes with multiplex CARS microscopy. J. Phys. Chem. B 106:3715-23
22. Rinia HA, Bonn M, Müller M. 2006. Quantitative multiplex CARS spectroscopy in congested spectral regions. J. Phys. Chem. B 110:4472-79
23. Kee TW, Zhao H, Cicerone MT. 2006. One-laser interferometric broadband coherent anti-Stokes Raman scattering. Opt. Express 14:3631-40
24. Nan X, Cheng J-X, Xie XS. 2003. Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy. J. Lipid Res. 44:2202-8
25. Rinia HA, Burger KNJ, Bonn M, Müller M. 2008. Quantitative label-free imaging of lipid composition and packing of individual cellular lipid droplets using multiplexCARSmicroscopy. Biophys. J. 95:4908-14
26. Wang H, Fu Y, Zickmund P, Shi R, Cheng J-X. 2005. Coherent anti-stokes Raman scattering imaging of axonal myelin in live spinal tissues. Biophys. J. 89:581-91
27. Bélanger E, Henry FP, Vallée R, Randolph MA, Kochevar IE, et al. 2011. In vivo evaluation of demyelination and remyelination in a nerve crush injury model. Biomed. Opt. Express 2:2698-708
28. Shi Y, Zhang D, Huff TB, Wang X, Shi R, et al. 2011. Longitudinal in vivo coherent anti-Stokes Raman scattering imaging of demyelination and remyelination in injured spinal cord. J. Biomed. Opt. 16:1060121
29. Cheng J-X. 2007. Coherent anti-Stokes Raman scattering microscopy. Appl. Spectrosc. 61:197-208
30. Müller M, Zumbusch A. 2007. Coherent anti-Stokes Raman scattering microscopy. ChemPhysChem 8:2156-70
31. Evans CL, Xie XS. 2008. Coherent anti-Stokes Raman scattering microscopy: chemical imaging for biology and medicine. Annu. Rev. Anal. Chem. 1:883-909
32. Zumbusch A, Langbein W, Borri P. 2013. Nonlinear vibrational microscopy applied to lipid biology. Prog. Lipid Res. 52:615-32
33. Parekh SH, Lee YJ, Aamer KA, Cicerone MT. 2010. Label-free cellular imaging by broadband coherent anti-Stokes Raman scattering microscopy. Biophys. J. 99:2695-704
34. Camp CH Jr, Lee YJ, Heddleston JM, Hartshorn CM, Hight Walker AR, et al. 2014. High-speed coherent Raman fingerprint imaging of biological tissues. Nat. Photonics 8:627-34
35. Di Napoli C, Pope I, Masia F, Watson P, Langbein W, Borri P. 2014. Hyperspectral and differential CARS microscopy for quantitative chemical imaging in human adipocytes. Biomed. Opt. Express 5:1378-90
36. Pegoraro AF, Slepkov AD, Ridsdale A, Moffatt DJ, Stolow A. 2014. Hyperspectral multimodal CARS microscopy in the fingerprint region. J. Biophotonics 7:49-58
37. Woodbury EJ, Ng WK. 1962. Ruby operation in the near IR. Proc. Inst. Radio Eng. 50:2367
38. Ploetz E, Laimgruber S, Berner S, Zinth W, Gilch P. 2007. Femtosecond stimulated Ramanmicroscopy. Appl. Phys. B 87:389-93
39. Freudiger CW, Min W, Saar BG, Lu S, Holtom GR, et al. 2008. Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy. Science 322:1857-61
40. Ozeki Y, Dake F, Kajiyama Si, Fukui K, Itoh K. 2009. Analysis and experimental assessment of the sensitivity of stimulated Raman scattering microscopy. Opt. Express 17:3651-58
41. Nandakumar P, Kovalev A, Volkmer A. 2009. Vibrational imaging based on stimulated Raman scattering microscopy. New J. Phys. 11:033026
42. Zhang D, Slipchenko MN, Cheng J-X. 2011. Highly sensitive vibrational imaging by femtosecond pulse stimulated Raman loss. J. Phys. Chem. Lett. 2:1248-53
43. Andresen ER, Berto P, RigneaultH. 2011. Stimulated Raman scattering microscopy by spectral focusing and fiber-generated soliton as Stokes pulse. Opt. Lett. 36:2387-89
44. Beier HT, Noojin GD, Rockwell BA. 2011. Stimulated Raman scattering using a single femtosecond oscillator with flexibility for imaging and spectral applications. Opt. Express 19:18885-92
45. Ozeki Y, Umemura W, Sumimura K, Nishizawa N, Fukui K, Itoh K. 2012. Stimulated Raman hyperspectral imaging based on spectral filtering of broadband fiber laser pulses. Opt. Lett. 37:431-33
46. Suhalim JL, Chung C-Y, Lilledahl MB, Lim RS, Levi M, et al. 2012. Characterization of cholesterol crystals in atherosclerotic plaques using stimulated Raman scattering and second-harmonic generation microscopy. Biophys. J. 102:1988-95
47. Fu D, Holtom G, Freudiger C, Zhang X, Xie XS. 2012. Hyperspectral imaging with stimulated Raman scattering by chirped femtosecond lasers. J. Phys. Chem. B 117:4634-40
48. Zhang D, Wang P, Slipchenko MN, Ben-Amotz D, Weiner AM, Cheng J-X. 2012. Quantitative vibrational imaging by hyperspectral stimulated Raman scattering microscopy and multivariate curve resolution analysis. Anal. Chem. 85:98-106
49. ZhangD,Wang P, Slipchenko MN, Cheng J-X. 2014. Fast vibrational imaging of single cells and tissues by stimulated Raman scattering microscopy. Acc. Chem. Res. 47:2282-90
50. MinW, Freudiger CW, Lu S, Xie XS. 2011. Coherent nonlinear optical imaging: beyond fluorescence microscopy. Annu. Rev. Phys. Chem. 62:507-30
51. Beenakker C, Büttiker M. 1992. Suppression of shot noise in metallic diffusive conductors. Phys. Rev. B 46:1889
52. Nyquist H. 1928. Thermal agitation of electric charge in conductors. Phys. Rev. 32:110-13
53. Kato Y, Takuma H. 1971. Absolute measurement of Raman-scattering cross sections of liquids. J. Opt. Soc. Am. 61:347-50
54. Chung C-Y, Boik J, Potma EO. 2013. Biomolecular imaging with coherent nonlinear vibrational microscopy. Annu. Rev. Phys. Chem. 64:77-99
55. Saar BG, Freudiger CW, Reichman J, Stanley CM, Holtom GR, Xie XS. 2010. Video-rate molecular imaging in vivo with stimulated Raman scattering. Science 330:1368-70
56. Dudovich N, Oron D, Silberberg Y. 2002. Single-pulse coherently controlled nonlinear Raman spectroscopy and microscopy. Nature 418:512-14
57. Bremer MT, Dantus M. 2013. Standoff explosives trace detection and imaging by selective stimulated Raman scattering. Appl. Phys. Lett. 103:061119
58. Heinrich C, Bernet S, Ritsch-Marte M. 2004. Wide-field coherent anti-Stokes Raman scattering microscopy. Appl. Phys. Lett. 84:816-18
59. Cheng J-X, Volkmer A, Book LD, Xie XS. 2001. An epi-detected coherent anti-Stokes Raman scattering (E-CARS) microscope with high spectral resolution and high sensitivity. J. Phys. Chem. B 105:1277-80
60. Ganikhanov F, Carrasco S, Sunney Xie X, Katz M, Seitz W, Kopf D. 2006. Broadly tunable dualwavelength light source for coherent anti-Stokes Raman scattering microscopy. Opt. Lett. 31:1292-94
61. Bonn M, Müller M, Rinia HA, Burger KNJ. 2009. Imaging of chemical and physical state of individual cellular lipid droplets using multiplex CARS microscopy. J. Raman Spectrosc. 40:763-69
62. Bégin S, Burgoyne B, Mercier V, Villeneuve A, Vallée R, Coté D. 2011. Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system. Biomed. Opt. Express 2:1296-306
63. Garbacik ET, Herek JL, Otto C, Offerhaus HL. 2012. Rapid identification of heterogeneous mixture components with hyperspectral coherent anti-Stokes Raman scattering imaging. J. Raman Spectrosc. 43:651-55
64. Hellerer T, Enejder AMK, Zumbusch A. 2004. Spectral focusing: high spectral resolution spectroscopy with broad-bandwidth laser pulses. Appl. Phys. Lett. 85:25-27
65. Rocha-Mendoza I, Langbein W, Borri P. 2008. Coherent anti-Stokes Raman microspectroscopy using spectral focusing with glass dispersion. Appl. Phys. Lett. 93:201103
66. Langbein W, Rocha-Mendoza I, Borri P. 2009. Coherent anti-Stokes Raman micro-spectroscopy using spectral focusing: theory and experiment. J. Raman Spectrosc. 40:800-8
67. Pegoraro AF, Ridsdale A, Moffatt DJ, Jia Y, Pezacki JP, Stolow A. 2009. Optimally chirped multimodal CARS microscopy based on a single Ti:sapphire oscillator. Opt. Express 17:2984-96
68. Chen B-C, Sung J, Wu X, Lim S-H. 2011. Chemical imaging and microspectroscopy with spectral focusing coherent anti-Stokes Raman scattering. J. Biomed. Opt. 16:021112
69. Masia F, Glen A, Stephens P, Borri P, Langbein W. 2013. Quantitative chemical imaging and unsupervised analysis using hyperspectral coherent anti-Stokes Raman scattering microscopy. Anal. Chem. 85:10820-28
70. Volkmer A, Book LD, Xie XS. 2002. Time-resolved coherent anti-Stokes Raman scattering microscopy: imaging based on Raman free induction decay. Appl. Phys. Lett. 80:1505-7
71. Ogilvie JP, Beaurepaire E, Alexandrou A, Joffre M. 2006. Fourier-transform coherent anti-Stokes Raman scattering microscopy. Opt. Lett. 31:480-82
72. Vartiainen EM, Rinia HA, Müller M, Bonn M. 2006. Direct extraction of Raman line-shapes from congested CARS spectra. Opt. Express 14:3622-30
73. Liu Y, Lee YJ, Cicerone MT. 2009. Broadband CARS spectral phase retrieval using a time-domain Kramers-Kronig transform. Opt. Lett. 34:1363-65
74. Marks DL, Boppart SA. 2004. Nonlinear interferometric vibrational imaging. Phys. Rev. Lett. 92:123905
75. Slipchenko MN, Oglesbee RA, Zhang D, Wu W, Cheng J-X. 2012. Heterodyne detected nonlinear optical imaging in a lock-in free manner. J. Biophotonics 5:801-7
76. Wang K, Zhang D, Charan K, Slipchenko MN, Wang P, et al. 2013. Time-lens based hyperspectral stimulated Raman scattering imaging and quantitative spectral analysis. J. Biophotonics 6:815-20
77. Wang P, Li J, Wang P, Hu CR, Zhang D, et al. 2013. Label-free quantitative imaging of cholesterol in intact tissues by hyperspectral stimulated Raman scattering microscopy. Angew. Chem. Int. Ed. 52:13042-46
78. Fu D, Zhou J, Zhu WS, Manley PW, Wang YK, et al. 2014. Imaging the intracellular distribution of tyrosine kinase inhibitors in living cells with quantitative hyperspectral stimulated Raman scattering. Nat. Chem. 6:614-22
79. Fu D, Lu F-K, Zhang X, Freudiger C, Pernik DR, et al. 2012. Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy. J. Am. Chem. Soc. 134:3623-26
80. Marx B, Czerwinski L, Light R, Somekh M, Gilch P. 2014. Multichannel detectors for femtosecond stimulated Raman microscopy-ideal and real ones. J. Raman Spectrosc. 45:521-27
81. Rock W, Bonn M, Parekh SH. 2013. Near shot-noise limited hyperspectral stimulated Raman scattering spectroscopy using low energy lasers and a fast CMOS array. Opt. Express 21:15113-20
82. Seto K, Okuda Y, Tokunaga E, Kobayashi T. 2013. Development of a multiplex stimulated Raman microscope for spectral imaging through multi-channel lock-in detection. Rev. Sci. Instrum. 84:083705
83. Liao C-S, Slipchenko MN, Wang P, Li J, Lee S-Y, et al. 2015. Microsecond scale vibrational spectroscopic imaging by multiplex stimulated Raman scattering microscopy. Light Sci. Appl. 4:e265
84. Le TT, Yue S, Cheng J-X. 2010. Shedding new light on lipid biology with coherent anti-Stokes Raman scattering microscopy. J. Lipid Res. 51:3091-102
85. Yue S, Slipchenko MN, Cheng J-X. 2011. Multimodal nonlinear optical microscopy. Laser Photonics Rev. 5:496-512
86. Pezacki JP, Blake JA, Danielson DC, Kennedy DC, Lyn RK, Singaravelu R. 2011. Chemical contrast for imaging living systems: molecular vibrations drive CARS microscopy. Nat. Chem. Biol. 7:137-45
87. Suhalim JL, Boik JC, Tromberg BJ, Potma EO. 2012. The need for speed. J. Biophotonics 5:387-95
88. Paar M, Jüngst C, Steiner NA, Magnes C, Sinner F, et al. 2012. Remodeling of lipid droplets during lipolysis and growth in adipocytes. J. Biol. Chem. 287:11164-73
89. Fussell A, Garbacik E, Offerhaus H, Kleinebudde P, Strachan C. 2013. In situ dissolution analysis using coherent anti-Stokes Raman scattering (CARS) and hyperspectral CARS microscopy. Eur. J. Pharm. Biopharm. 85:1141-47
90. Le TT, Huff TB, Cheng J-X. 2009. Coherent anti-Stokes Raman scattering imaging of lipids in cancer metastasis. BMC Cancer 9:42
91. Mitra R, Chao O, Urasaki Y, Goodman OB, Le TT. 2012. Detection of lipid-rich prostate circulating tumour cells with coherent anti-Stokes Raman scattering microscopy. BMC Cancer 12:540
92. Okuno M, Kano H, Fujii K, Bito K, Naito S, et al. 2014. Surfactant uptake dynamics in mammalian cells elucidated with quantitative coherent anti-Stokes Raman scattering microspectroscopy. PLOS ONE 9:e93401
93. Hellerer T, Axäng C, Brackmann C, Hillertz P, Pilon M, Enejder A. 2007. Monitoring of lipid storage in Caenorhabditis elegans using coherent anti-Stokes Raman scattering (CARS) microscopy. PNAS 104:14658-63
94. Yen K, Le TT, Bansal A, Narasimhan SD, Cheng J-X, TissenbaumHA. 2010. A comparative study of fat storage quantitation in nematode Caenorhabditis elegans using label and label-free methods. PLOS ONE 5:e12810
95. Le TT, Duren HM, Slipchenko MN, Hu C-D, Cheng J-X. 2010. Label-free quantitative analysis of lipid metabolism in living Caenorhabditis elegans. J. Lipid Res. 51:672-77
96. Fu Y, Wang H, Huff TB, Shi R, Cheng J-X. 2007. Coherent anti-Stokes Raman scattering imaging of myelin degradation reveals a calcium-dependent pathway in Lyso-PtdCho-induced demyelination. J. Neurosci. Res. 85:2870-81
97. Shi Y, Kim S, Huff TB, Borgens RB, Park K, et al. 2010. Effective repair of traumatically injured spinal cord by nanoscale block copolymer micelles. Nat. Nanotechnol. 5:80-87
98. Huff TB, Cheng J-X. 2007. In vivo coherent anti-Stokes Raman scattering imaging of sciatic nerve tissue. J. Microsc. 225:175-82
99. Jung Y, Ng JH, Keating CP, Senthil-Kumar P, Zhao J, et al. 2014. Comprehensive evaluation of peripheral nerve regeneration in the acute healing phase using tissue clearing and optical microscopy in a rodent model. PLOS ONE 9:e94054
100. Lim RS, Suhalim JL, Miyazaki-Anzai S, Miyazaki M, Levi M, et al. 2011. Identification of cholesterol crystals in plaques of atherosclerotic mice using hyperspectral CARS imaging. J. Lipid Res. 52:2177-86
101. Meyer T, Chemnitz M, Baumgartl M, Gottschall T, Pascher TR, et al. 2013. Expanding multimodal microscopy by high spectral resolution coherent anti-StokesRaman scattering imaging for clinical disease diagnostics. Anal. Chem. 85:6703-15
102. Breunig HG, Weinigel M, Bückle R, Kellner-Höfer M, Lademann J, et al. 2013. Clinical coherent anti-Stokes Raman scattering and multiphoton tomography of human skin with a femtosecond laser and photonic crystal fiber. Laser Phys. Lett. 10:025604
103. Galli R, Uckermann O, Koch E, Schackert G, Kirsch M, Steiner G. 2014. Effects of tissue fixation on coherent anti-Stokes Raman scattering images of brain. J. Biomed. Opt. 19:071402
104. Cheng J-X, Pautot S, Weitz DA, Xie XS. 2003. Ordering of water molecules between phospholipid bilayers visualized by coherent anti-Stokes Raman scattering microscopy. PNAS 100:9826-30
105. Zimmerley M, Younger R, Valenton T, Oertel DC,Ward JL, Potma EO. 2010. Molecular orientation in dry and hydrated cellulose fibers: A coherent anti-Stokes Raman scattering microscopy study. J. Phys. Chem. B 114:10200-8
106. Kennedy AP, Sutcliffe J, Cheng J-X. 2005. Molecular composition and orientation in myelin figures characterized by coherent anti-Stokes Raman scattering microscopy. Langmuir 21:6478-86
107. de Vito G, Bifone A, Piazza V. 2012. Rotating-polarization CARS microscopy: combining chemical and molecular orientation sensitivity. Opt. Express 20:29369-77
108. DouW, Zhang D, Jung Y, Cheng J-X, Umulis DM. 2012. Label-free imaging of lipid-droplet intracellular motion in early Drosophila embryos using femtosecond-stimulated Raman loss microscopy. Biophys. J. 102:1666-75
109. Wang MC, Min W, Freudiger CW, Ruvkun G, Xie XS. 2011. RNAi screening for fat regulatory genes with SRS microscopy. Nat. Methods 8:135-38
110. Zhang X, Roeffaers MBJ, Basu S, Daniele JR, Fu D, et al. 2012. Label-free live-cell imaging of nucleic acids using stimulated Raman scattering microscopy. ChemPhysChem 13:1054-59
111. Saar BG, Zeng Y, Freudiger CW, Liu Y-S, Himmel ME, et al. 2010. Label-free, real-time monitoring of biomass processing with stimulated Raman scattering microscopy. Angew. Chem. Int. Ed. 49:5476-79
112. Wei L, Yu Y, Shen Y, Wang MC, Min W. 2013. Vibrational imaging of newly synthesized proteins in live cells by stimulated Raman scattering microscopy. PNAS 110:11226-31
113. Wei L, Hu F, Shen Y, Chen Z, Yu Y, et al. 2014. Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering. Nat. Methods 11:410-12
114. Hong S, Chen T, Zhu Y, Li A, Huang Y, Chen X. 2014. Live-cell stimulated Raman scattering imaging of alkyne-tagged biomolecules. Angew. Chem. Int. Ed. 126:5937-41
115. Shen Y, Xu F,Wei L, Hu F, MinW. 2014. Live-cell quantitative imaging of proteome degradation by stimulated Raman scattering. Angew. Chem. Int. Ed. 53:5596-99
116. Hu F, Wei L, Zheng C, Shen Y, Min W. 2014. Live-cell vibrational imaging of choline metabolites by stimulated Raman scattering coupled with isotope-based metabolic labeling. Analyst 139:2312-17
117. Wei L, Shen Y, Xu F, Hu F, Harrington JK, et al. 2015. Imaging complex protein metabolism in live organisms by stimulated Raman scattering microscopy with isotope labeling. ACS Chem. Biol. 10:901-8
118. Chen Z, PaleyDW, Wei L,Weisman AL, Friesner RA, et al. 2014. Multicolor live-cell chemical imaging by isotopically edited alkyne vibrational palette. J. Am. Chem. Soc. 136:8027-33
119. Lee HJ, Zhang W, Zhang D, Yang Y, Liu B, et al. 2015. Assessing cholesterol storage in live cells and C. elegans by stimulated Raman scattering imaging of phenyl-diyne cholesterol. Sci. Rep. 5:7930
120. HuC-R, ZhangD, SlipchenkoMN,Cheng J-X,HuB. 2014. Label-free real-time imaging ofmyelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy. J. Biomed. Opt. 19:086005
121. Wang P, Liu B, Zhang D, Belew MY, Tissenbaum HA, Cheng J-X. 2014. Imaging lipid metabolism in live Caenorhabditis elegans using fingerprint vibrations. Angew. Chem. Int. Ed. 53:11787-92
122. Yue S, Li J, Lee S-Y, Lee HJ, Shao T, et al. 2014. Cholesteryl ester accumulation induced by PTEN loss and PI3K/AKT activation underlies human prostate cancer aggressiveness. Cell Metab. 19:393-406
123. Ji M, Orringer DA, Freudiger CW, Ramkissoon S, Liu X, et al. 2013. Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy. Sci. Transl. Med. 5:201ra119
124. FreudigerCW,Pfannl R, Orringer DA, Saar BG, JiM, et al. 2012. Multicolored stain-free histopathology with coherent Raman imaging. Lab. Investig. 92:1492-502
125. Huff TB, Shi Y, Fu Y, Wang H, Cheng J-X. 2008. Multimodal nonlinear optical microscopy and applications to central nervous system imaging. IEEE J. Sel. Top. Quantum Electron. 14:4-9
126. Chen H, Wang H, Slipchenko MN, Jung Y, Shi Y, et al. 2009. A multimodal platform for nonlinear optical microscopy and microspectroscopy. Opt. Express 17:1282-90
127. Zhai Y-H, GoulartC, Sharping JE, WeiH,Chen S, et al. 2011. Multimodal coherent anti-Stokes Raman spectroscopic imaging with a fiber optical parametric oscillator. Appl. Phys. Lett. 98:191106
128. Meyer T, Baumgartl M, Gottschall T, Pascher T,Wuttig A, et al. 2013. A compact microscope setup for multimodal nonlinear imaging in clinics and its application to disease diagnostics. Analyst 138:4048-57
129. Le TT, Rehrer CW,Huff TB, NicholsMB, Camarillo IG, Cheng J-X. 2007. Nonlinear optical imaging to evaluate the impact of obesity on mammary gland and tumor stroma. Mol. Imaging 6:205
130. Wang H-W, Langohr IM, Sturek M, Cheng J-X. 2009. Imaging and quantitative analysis of atherosclerotic lesions by CARS-based multimodal nonlinear optical microscopy. Arterioscler. Thromb. Vasc. Biol. 29:1342-48
131. Mansfield J, Yu J, Attenburrow D,Moger J, Tirlapur U, et al. 2009. The elastin network: its relationship with collagen and cells in articular cartilage as visualized bymultiphoton microscopy. J. Anat. 215:682-91
132. Vogler N, Medyukhina A, Latka I, Kemper S, Böhm M, et al. 2011. Towards multimodal nonlinear optical tomography-experimental methodology. Laser Phys. Lett. 8:617-24
133. Mouras R, Bagnaninchi PO, Downes AR, Elfick AP. 2012. Label-free assessment of adipose-derived stem cell differentiation using coherent anti-Stokes Raman scattering and multiphoton microscopy. J. Biomed. Opt. 17:116011
134. Mortati L, Divieto C, Sassi MP. 2012. CARS and SHG microscopy to follow collagen production in living human corneal fibroblasts and mesenchymal stem cells in fibrin hydrogel 3D cultures. J. Raman Spectrosc. 43:675-80
135. Segawa H, Okuno M, Kano H, Leproux P, Couderc V, Hamaguchi H-O. 2012. Label-free tetra-modal molecular imaging of living cells with CARS, SHG, THG and TSFG (coherent anti-Stokes Raman scattering, second harmonic generation, third harmonic generation and third-order sum frequency generation). Opt. Express 20:9551-57
136. Garrett NL, Lalatsa A, Uchegbu I, Schätzlein A, Moger J. 2012. Exploring uptake mechanisms of oral nanomedicines using multimodal nonlinear optical microscopy. J. Biophotonics 5:458-68
137. Hoover EE, Squier JA. 2013. Advances in multiphoton microscopy technology. Nat. Photonics 7:93-101
138. Jung Y, Slipchenko MN, Liu CH, Ribbe AE, Zhong Z, et al. 2010. Fast detection of the metallic state of individual single-walled carbon nanotubes using a transient-Absorption optical microscope. Phys. Rev. Lett. 105:217401
139. Tong L, Liu Y, Dolash BD, Jung Y, Slipchenko MN, et al. 2012. Label-free imaging of semiconducting andmetallic carbon nanotubes in cells andmice using transient absorption microscopy. Nat. Nanotechnol. 7:56-61
140. Wang H, Huff TB,Zweifel DA, HeW,Low PS, et al. 2005. In vitro and in vivo two-photon luminescence imaging of single gold nanorods. PNAS 102:15752-56
141. Tong L, Cobley CM, Chen J, Xia Y, Cheng J-X. 2010. Bright three-photon luminescence from gold/silver alloyed nanostructures for bioimaging with negligible photothermal toxicity. Angew. Chem. Int. Ed. 49:3485-88
142. KimH, Sheps T, CollinsPG, PotmaEO. 2009. Nonlinear optical imaging of individual carbon nanotubes with four-wave-mixing microscopy. Nano Lett. 9:2991-95
143. MinW, Lu S, Chong S, Roy R, Holtom GR, Xie XS. 2009. Imaging chromophores with undetectable fluorescence by stimulated emission microscopy. Nature 461:1105-9
144. Wei L,Min W. 2012. Pump-probe optical microscopy for imaging nonfluorescent chromophores. Anal. Bioanal. Chem. 403:2197-202
145. MinW, Lu S, Rueckel M, Holtom GR, Xie XS. 2009. Near-degenerate four-wave-mixing microscopy. Nano Lett. 9:2423-26
146. Lu S, Min W, Chong S, Holtom GR, Xie XS. 2010. Label-free imaging of heme proteins with twophoton excited photothermal lens microscopy. Appl. Phys. Lett. 96:113701
147. Moger J, Garrett N, Begley D, Mihoreanu L, Lalatsa A, et al. 2012. Imaging cortical vasculature with stimulated Raman scattering and two-photon photothermal lensing microscopy. J. Raman Spectrosc. 43:668-74
148. Slepkov AD, Ridsdale A, Wan H-N,Wang M-H, Pegoraro AF, et al. 2011. Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy. J. Biomed. Opt. 16:021103
149. Slipchenko MN, Le TT, Chen H, Cheng J-X. 2009. High-speed vibrational imaging and spectral analysis of lipid bodies by compound Raman microscopy. J. Phys. Chem. B 113:7681-86
150. Slipchenko MN, Chen H, Ely DR, Jung Y, Carvajal MT, Cheng J-X. 2010. Vibrational imaging of tablets by epi-detected stimulated Raman scattering microscopy. Analyst 135:2613-19
151. Yue S, Cárdenas-Mora JM, Chaboub LS, Lelièvre SA, Cheng J-X. 2012. Label-free analysis of breast tissue polarity by Raman imaging of lipid phase. Biophys. J. 102:1215-23
152. Galli R, Uckermann O, Winterhalder MJ, Sitoci-Ficici KH, Geiger KD, et al. 2012. Vibrational spectroscopic imaging and multiphoton microscopy of spinal cord injury. Anal. Chem. 84:8707-14
153. Le TT, Ziemba A, Urasaki Y, Brotman S, PizzornoG. 2012. Label-free evaluation of hepatic microvesicular steatosis with multimodal coherent anti-Stokes Raman scattering microscopy. PLOS ONE 7:e51092
154. Chong A, Renninger WH, Wise FW. 2007. All-normal-dispersion femtosecond fiber laser with pulse energy above 20 nJ. Opt. Lett. 32:2408-10
155. Kieu K, Renninger W, Chong A,Wise F. 2009. Sub-100 fs pulses at watt-level powers from a dissipativesoliton fiber laser. Opt. Lett. 34:593-95
156. ChichkovNB, Hapke C,Neumann J, KrachtD,Wandt D, MorgnerU. 2012. Pulse duration and energy scaling of femtosecond all-normal dispersion fiber oscillators. Opt. Express 20:3844-52
157. Tang S, Liu J, Krasieva TB, Chen Z, Tromberg BJ. 2009. Developing compact multiphoton systems using femtosecond fiber lasers. J. Biomed. Opt. 14:030508
158. Nie B, Saytashev I, Chong A, Liu H, Arkhipov SN, et al. 2012. Multimodal microscopy with sub-30 fs Yb fiber laser oscillator. Biomed. Opt. Express 3:1750-56
159. Baumgartl M, Lecaplain C, Hideur A, Limpert J, Tünnermann A. 2012. 66 W average power from a microjoule-class sub-100 fs fiber oscillator. Opt. Lett. 37:1640-42
160. Paulsen HN, Hilligse KM, Thøgersen J, Keiding SR, Larsen JJ. 2003. Coherent anti-Stokes Raman scattering microscopy with a photonic crystal fiber based light source. Opt. Lett. 28:1123-25
161. Krauss G, Hanke T, Sell A, Träutlein D, Leitenstorfer A, et al. 2009. Compact coherent anti-Stokes Raman scattering microscope based on a picosecond two-color Er:fiber laser system. Opt. Lett. 34:2847-49
162. Lefrancois S, Fu D, Holtom GR, Kong L, Wadsworth WJ, et al. 2012. Fiber four-wave mixing source for coherent anti-Stokes Raman scattering microscopy. Opt. Lett. 37:1652-54
163. Lamb ES, Lefrancois S, JiM,Wadsworth WJ, Sunney Xie X, Wise FW. 2013. Fiber optical parametric oscillator for coherent anti-Stokes Raman scattering microscopy. Opt. Lett. 38:4154-57
164. Nose K, Ozeki Y,Kishi T, Sumimura K, NishizawaN, et al. 2012. Sensitivity enhancement of fiber-laserbased stimulated Raman scattering microscopy by collinear balanced detection technique. Opt. Express 20:13958-65
165. Nose K, Kishi T, Ozeki Y, Kanematsu Y, Takata H, et al. 2014. Stimulated Raman spectral microscope using synchronized Er-And Yb-fiber lasers. Jpn. J. Appl. Phys. 53:052401
166. Freudiger CW, Yang W, Holtom GR, Peyghambarian N, Xie XS, Kieu KQ. 2014. Stimulated Raman scattering microscopy with a robust fibre laser source. Nat. Photonics 8:153-59
167. Masihzadeh O, Ammar DA, Kahook MY, Lei TC. 2013. Coherent anti-Stokes Raman scattering (CARS) microscopy: A novel technique for imaging the retina. Investig. Ophthalmol. Vis. Sci. 54:3094-101
168. Wu Y-M, Chen H-C, Chang W-T, Jhan J-W, Lin H-L, Liau I. 2009. Quantitative assessment of hepatic fat of intact liver tissues with coherent anti-Stokes Raman scattering microscopy. Anal. Chem. 81:1496-504
169. Gao L, Zhou H, Thrall MJ, Li F, Yang Y, et al. 2011. Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy. Biomed. Opt. Express 2:915-26
170. Saar BG, Contreras-Rojas LR, Xie XS, Guy RH. 2011. Imaging drug delivery to skin with stimulated Raman scattering microscopy. Mol. Pharm. 8:969-75
171. Belsey NA, Garrett NL, Contreras-Rojas LR, Pickup-Gerlaugh AJ, Price GJ, et al. 2014. Evaluation of drug delivery to intact and porated skin by coherent Raman scattering and fluorescence microscopies. J. Control. Release 174:37-42
172. Breunig HG, Bückle R, Kellner-Höfer M, Weinigel M, Lademann J, et al. 2012. Combined in vivo multiphoton and CARS imaging of healthy and disease-Affected human skin. Microsc. Res. Tech. 75:492-98
173. Zimmerley M, McClureRA, Choi B, PotmaEO. 2009. Following dimethyl sulfoxide skin optical clearing dynamics with quantitative nonlinear multimodal microscopy. Appl. Opt. 48:D79-87
174. König K, Breunig H, Bückle R, Kellner-Höfer M, Weinigel M, et al. 2011. Optical skin biopsies by clinical CARS and multiphoton fluorescence/SHG tomography. Laser Phys. Lett. 8:465-68
175. Smith B, NajiM,Murugkar S, Alarcon E, Brideau C, et al. 2013. Portable, miniaturized, fibre delivered, multimodal CARS exoscope. Opt. Express 21:17161-75
176. MittalR,Balu M, Krasieva T, PotmaEO, Elkeeb L, et al. 2013. Evaluation of stimulated Raman scattering microscopy for identifying squamous cell carcinoma in human skin. Laser Surg. Med. 45:496-502
177. Drutis D, Hancewicz T, Pashkovski E, Feng L, Mihalov D, et al. 2014. Three-dimensional chemical imaging of skin using stimulated Raman scattering microscopy. J. Biomed. Opt. 19:111604
178. Rivera DR, Brown CM, Ouzounov DG, Pavlova I, Kobat D, et al. 2011. Compact and flexible raster scanning multiphoton endoscope capable of imaging unstained tissue. PNAS 108:17598-603
179. Zhang Y, Akins ML, Murari K, Xi J, Li M-J, et al. 2012. A compact fiber-optic SHG scanning endomicroscope and its application to visualize cervical remodeling during pregnancy. PNAS 109:12878-83
180. Légaré F, Evans CL, Ganikhanov F, Xie XS. 2006. Towards CARS endoscopy. Opt. Express 14:4427-32
181. Saar BG, Johnston RS, Freudiger CW, Xie XS, Seibel EJ. 2011. Coherent Raman scanning fiber endoscopy. Opt. Lett. 36:2396-98
182. Liu Z, Wang Z, Wang X, Xu X, Chen X, et al. 2013. Fiber bundle based probe with polarization for coherent anti-Stokes Raman scattering microendoscopy imaging. Proc. SPIE 8588:85880F. doi: 10.1117/12.2004229