Multicolored stain-free histopathology with coherent Raman imaging

Laboratory Investigation

Volumn 92, Issue 10, 2012, Pages 1492-1502

  Freudiger, Christian W ^a   Pfannl, Rolf ^b   Orringer, Daniel A ^c,d,e   Saar, Brian G ^a,k   Ji, Minbiao ^a   Zeng, Qing ^d   Ottoboni, Linda ^c   Ying, Wei ^f   Waeber, Christian ^f   Sims, John R ^f   De Jager, Philip L ^c,d,g   Sagher, Oren ^e   Philbert, Martin A ^h   Xu, Xiaoyin ^d   Kesari, Santosh ^i,j   Xie, X Sunney ^a   Young, Geoffrey S ^d  

^a HARVARD UNIVERSITY   (United States)

^b TUFTS MEDICAL CENTER   (United States)

^c BRIGHAM AND WOMEN S HOSPITAL   (United States)

^d HARVARD MEDICAL SCHOOL   (United States)

^e UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^f MASSACHUSETTS GENERAL HOSPITAL   (United States)

^g BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^h UNIVERSITY OF MICHIGAN   (United States)

ⁱ DANA FARBER CANCER INSTITUTE   (United States)

^j UNIVERSITY OF CALIFORNIA   (United States)

^k MIT LINCOLN LABORATORY   (United States)

more..

Author keywords

CARS; Coherent anti stokes Raman scattering; histology; in vivo microscopy; SRS; stimulated Raman scattering

Indexed keywords

HEMOGLOBIN; LIPID; PROTEIN;

ABSORPTION; ALLERGIC ENCEPHALOMYELITIS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN METASTASIS; BREAST CANCER; CENTRAL NERVOUS SYSTEM DISEASE; CEREBELLUM; CEREBROVASCULAR ACCIDENT; COHERENT RAMAN IMAGING; COLOR; CONTRAST ENHANCEMENT; CONTROLLED STUDY; CORPUS CALLOSUM; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; EX VIVO STUDY; GLIOMA; HISTOPATHOLOGY; HUMAN; HUMAN CELL; LASER MICROSCOPY; MOUSE; NONHUMAN; PRIORITY JOURNAL; RAMAN SPECTROMETRY; TISSUE FIXATION;

EID: 84866872022     PISSN: 00236837     EISSN: 15300307     Source Type: Journal    
DOI: 10.1038/labinvest.2012.109     Document Type: Article

References (42)

1. Zumbusch A, Holtom GR, Xie XS. Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering. Phys Rev Lett 1999;82:4142-4145.
2. Evans CL, Xie XS. Coherent anti-Stokes Raman scattering microscopy: chemical imaging for biology and medicine. Annu Rev Anal Chem 2008;1:27.
3. Ploetz E, Laimgruber S, Berner S, et al. Femtosecond stimulated Raman microscopy. Appl Phys B 2007;87:389-393.
4. Freudiger CW, Min W, Saar BG, et al. Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy. Science 2008;322:1857-1861.
5. Ozeki Y, Dake F, Kajiyama S, et al. Analysis and experimental assessment of the sensitivity of stimulated Raman scattering microscopy. Optics Express 2009;17:3651-3658.
6. Nandakumar P, Kovalev A, Volkmer A. Vibrational imaging based on stimulated Raman scattering microscopy. New J Phys 2009;11 (article: 033026); http://iopscience.iop.org/1367-2630/11/3/033026/pdf/1367-2630-11-3-033026.pdf.
7. Evans CL, Potma EO, Puoris'haag M, et al. Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy. Proc Natl Acad Sci USA 2005;102:16807-16812.
8. Saar BG, Freudiger CW, Reichman J, et al. Video-rate molecular imaging in vivo with stimulated Raman scattering. Science 2010;330: 1368-1370.
9. Fu D, Ye T, Matthews TE, et al. Two-color, two-photon, and excitedstate absorption microscopy. J Biomed Opt 2007;12:54004-54009.
10. Cheng JX, Volkmer A, Book LD, et al. An epi-detected coherent anti-Stokes raman scattering (E-CARS) microscope with high spectral resolution and high sensitivity. J Phys Chem B 2001;105: 1277-1280.
11. Nan XL, Potma EO, Xie XS. Nonperturbative chemical imaging of organelle transport in living cells with coherent anti-Stokes Raman scattering microscopy. Biophysical J 2006;91:728-735.
12. Fu Y, Wang HF, Shi RY, et al. Characterization of photodamage in coherent anti-Stokes Raman scattering microscopy. Optics Express 2006;14:3942-3951.
13. Fu Y, Wang HF, Huff TB, et al. Coherent anti-Stokes Raman scattering imaging of myelin degradation reveals a calcium-dependent pathway in lyso-PtdCho-induced demyelination. J Neurosci Res 2007;85: 2870-2881.
14. Fu Y, Huff TB, Wang HW, et al. Ex vivo and in vivo imaging of myelin fibers in mouse brain by coherent anti-Stokes Raman scattering microscopy. Optics Express 2008;16:19396-19409.
15. Henry FP, Cô té D, Randolph MA, et al. Real-Time in vivo assessment of the nerve microenvironment with coherent anti-Stokes Raman scattering microscopy. Plastic and Reconstructive Surgery 2009; 123:123s-130s.
16. Fu D, Ye T, Matthews TE, et al. High-resolution in vivo imaging of blood vessels without labeling. Opt Lett 2007;32:2641-2643.
17. Fu D, Lu FK, Zhang X, et al. Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy. J Am Chem Soc 2012;134:3623-3626.
18. Evans CL, Xu X, Kesari S, et al. Chemically-selective imaging of brain structures with CARS microscopy. Optics Express 2007;15:12076-12087.
19. Begin S, Belanger E, Laffray S, et al. In vivo optical monitoring of tissue pathologies and diseases with vibrational contrast. J Biophotonics 2009;2:632-642.
20. Chowdary PD, Jiang Z, Chaney EJ, et al. Molecular histopathology by spectrally reconstructed nonlinear interferometric vibrational imaging. Cancer Res 2010;70:9562-9569.
21. Meyer T, Bergner N, Bielecki C, et al. Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis (Journal Paper). J Biomed Opt 2011;16:021113.
22. Légaré F, Evans CL, Ganikhanov F, et al. Towards CARS endoscopy. Opt Express 2006;14:4427-4432.
23. Murugkar S, Smith B, Srivastava P, et al. Miniaturized multimodal CARS microscope based on MEMS scanning and a single laser source. Optics Express 2010;18:23796-23804.
24. Balu M, Liu G, Chen Z, et al. Fiber delivered probe for efficient CARS imaging of tissues. Optics Express 2010;18:2380-2388.
25. Saar BG, Johnston RS, Freudiger CW, et al. Coherent Raman scanning fiber endoscopy. Optics Letters 2011;36:2396-2398.
26. Zhang X, Roeffaers MB, Basu S, et al. Label-free live-cell imaging of nucleic acids using stimulated Raman scattering microscopy. ChemPhysChem 2012;13:1054-1059.
27. Slipchenko MN, Le TT, Chen HT, et al. High-speed vibrational imaging and spectral analysis of lipid bodies by compound Raman microscopy. J Phys Chem B 2009;113:7681-7686.
28. Wang HF, Fu Y, Zickmund P, et al. Coherent anti-stokes Raman scattering imaging of axonal myelin in live spinal tissues. Biophysical J 2005;89:581-591.
29. Matthews TE, Piletic IR, Selim MA, et al. Pump-probe imaging differentiates melanoma from melanocytic nevi. Science Translational Medicine 2011;3:71ra15.
30. Omuro AMP, Leite CC, Mokhtari K, et al. Pitfalls in the diagnosis of brain tumours. Lancet Neurology 2006;5:937-948.
31. Laffray S, Pagès S, Dufour H, et al. Adaptive movement compensation for in vivo imaging of fast cellular dynamics within a moving tissue. PloS One 2011;6:e19928.
32. Gremlich HU, Yan B. Infrared and Raman Spectroscopy of Biological Materials. Marcel Dekker, Inc.: New York, Vol. 24, 2001.
33. Master BR, So PTC. Biomedical Nonlinear Optical Microscopy. Oxford University Press, Inc.: New York, 2008.
34. Kö nig K, Ehlers A, Riemann I, et al. Clinical two-photon microendoscopy. Microsc Res Techn 2007;70:398-402.
35. Tearney GJ, Brezinski ME, Bouma BE, et al. In vivo endoscopic optical biopsy with optical coherence tomography. Science 1997;276: 2037-2039.
36. Tai S, Tsai TH, Lee WJ, et al. Optical biopsy of fixed human skin with backward-collected optical harmonics signals. Optics Express 2005;13:8231-8242.
37. Witte S, Negrean A, Lodder JC, et al. Label-free live brain imaging and targeted patching with third-harmonic generation microscopy. Proc Natl Acad of Sci 2011;108:5970.
38. Zipfel WR, Williams RM, Christie R, et al. Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation. Proc Natl Acad Sci USA 2003; 100:7075-7080.
39. Li CQ, Pastila RK, Pitsillides C, et al. Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence. Optics Express 2010;18:988-999.
40. Campagnola PJ, Millard AC, Terasaki M, et al. Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues. Biophysical J 2002;82:493-508.
41. Palero JA, de Bruijn HS, van der Ploeg van den Heuvel A, et al. Spectrally resolved multiphoton imaging of in vivo and excised mouse skin tissues. Biophysical J 2007;93:992-1007.
42. Kö nig K. Clinical multiphoton tomography. J Biophotonics 2008;1: 13-23.