Label-free biomedical imaging with high sensitivity by stimulated raman scattering microscopy

Science

Volumn 322, Issue 5909, 2008, Pages 1857-1861

  Freudiger, Christian W ^a,b   Min, Wei ^a   Saar, Brian G ^a   Lu, Sijia ^a   Holtom, Gary R ^a   He, Chengwei ^c   Tsai, Jason C ^d   Kang, Jing X ^c   Xie, X Sunney ^a  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD UNIVERSITY   (United States)

^c MASSACHUSETTS GENERAL HOSPITAL   (United States)

^d PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIMETHYL SULFOXIDE; DOCOSAHEXAENOIC ACID; ICOSAPENTAENOIC ACID; LIPID; OMEGA 3 FATTY ACID; RETINOIC ACID; SATURATED FATTY ACID; SKIN LIPID;

BRAIN; DRUG; FATTY ACID; IMAGING METHOD; RAMAN SPECTROSCOPY; SENSITIVITY ANALYSIS; SKIN;

ARTICLE; CHEMICAL BOND; CHEMICAL COMPOSITION; HYDROPHILICITY; IMAGING; MEDICAL INSTRUMENTATION; MICROSCOPY; NEUROIMAGING; NONHUMAN; PRIORITY JOURNAL; RAMAN SCATTERING MICROSCOPY; RAMAN SPECTROMETRY; SENSITIVITY ANALYSIS; SKIN CULTURE; THREE DIMENSIONAL IMAGING; VIBRATION;

ANIMALS; CELL LINE, TUMOR; CORPUS CALLOSUM; DIMETHYL SULFOXIDE; EICOSAPENTAENOIC ACID; EPIDERMIS; HUMANS; IMAGING, THREE-DIMENSIONAL; LIPIDS; MICE; MICROSCOPY; NEURONS; SENSITIVITY AND SPECIFICITY; SKIN; SPECTRUM ANALYSIS, RAMAN; TRETINOIN; VITAMIN A;

EID: 57849134248     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1165758     Document Type: Article

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32. We thank C. Ackermann and S. Zhang for advice on skin imaging; S. Kesari, X. Xu and the MCB Harvard Mouse Facilities for providing mouse tissue; J. Grice and M. Roberts for the loan of a sonicator; and G. Young, X. Xu, M. Rückel, and P. Sims for helpful discussions. C.W.F. and B.G.S. thank Boehringer Ingelheim Fonds for a Ph.D. Fellowship and the Army Research Office for a National Defense Science and Engineering Graduate Fellowship, respectively. J.X.K. acknowledges support from the NIH (grant CA113605). The Xie Group is grateful to the U.S. Department of Energy's Basic Energy Sciences program (DE-FG02-07ER15875) for supporting high-sensitivity Raman detection. The instrumentation development was supported by NSF (grant DBI-0649892), NIH Director's Pioneer Award (to X.S.X.), the Bill & Melinda Gates Foundation Gates Foundation, and Pfizer Global Medical. Harvard University has filed a patent application based on this work.