Hyperspectral and differential CARS microscopy for quantitative chemical imaging in human adipocytes

Biomedical Optics Express

Volumn 5, Issue 5, 2014, Pages 1378-1390

  Di Napoli, Claudia ^a   Pope, Iestyn ^a   Masia, Francesco ^b   Watson, Peter ^a   Langbein, Wolfgang ^b   Borri, Paola ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

^b CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL ANALYSIS; FACTORIZATION; LIPIDS; RAMAN SPECTROSCOPY; STEM CELLS;

CHEMICAL COMPONENT; COHERENT ANTI-STOKES RAMAN SCATTERING; FACTORIZATION METHODS; HYPERSPECTRAL IMAGING; LIPID COMPOSITION; MICRO SPECTROSCOPY; NONLINEAR BEHAVIOR; RATIOMETRIC ANALYSIS;

COHERENT SCATTERING;

FATTY ACID;

ADIPOCYTE; ADIPOSE DERIVED STEM CELL; ANTI STOKES RAMAN SCATTERING MICROSPECTROSCOPY; ARTICLE; FACTORIAL ANALYSIS; FREQUENCY ANALYSIS; HUMAN; HUMAN CELL; HYPERSPECTRAL IMAGING; IMAGING; LASER; QUANTITATIVE CHEMICAL IMAGING; RAMAN SPECTROMETRY; RATIOMETRIC ANALYSIS; SPECTROSCOPY; VIBRATIONAL FINGERPRINT REGION;

EID: 84899758926     PISSN: None     EISSN: 21567085     Source Type: Journal    
DOI: 10.1364/BOE.5.001378     Document Type: Article

References (23)

1. A. Zumbusch, W. Langbein, and P. Borri, "Nonlinear vibrational microscopy applied to lipid biology, " Prog. Lipid Res. 52, 615-632 (2013).
2. J. P. Pezacki, J. A. Blake, D. C. Danielson, D. C. Kennedy, R. K. Lyn, and R. Singaravelu, "Chemical contrast for imaging living systems: molecular vibrations drive cars microscopy, " Nat. Chem. Biol. 7, 137-145 (2011).
3. M. Digel, R. Ehehalt, and J. Füllekrug, "Lipid droplets lighting up: Insights from live microscopy, " FEBS Lett. 584, 2168-2175 (2010).
4. T. C. Walther and R. V. Farese Jr, "Lipid droplets and cellular lipid metabolism, " Annu. Rev. Biochem. 81, 687-714 (2012).
5. S. Fukumoto and T. Fujimoto, "Deformation of lipid droplets in fixed samples, " Histochem. Cell Biol. 118, 423-428 (2002).
6. X. Nan, J. X. Cheng, and X. S. Xie, "Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-stokes raman scattering microscopy, " J. Lipid Res. 44, 2202-2208 (2003).
7. Y. Ohsaki, Y. Shinohara, M. Suzuki, and T. Fujimoto, "A pitfall in using bodipy dyes to label lipid droplets for fluorescence microscopy, " Histochem. Cell Biol. 133, 477-480 (2010).
8. M. Paar, C. Jüngst, N. A. Steiner, C. Magnes, F. Sinner, D. Kolb, A. Lass, R. Zimmermann, A. Zumbusch, S. D. Kohlwein, and H. Wolinski, "Remodeling of lipid droplets during lipolysis and growth in adipocytes, " J. Biol. Chem. 287, 11164-11173 (2012).
9. E. M. Vartiainen, H. A. Rinia, M. Müller, and M. Bonn, "Direct extraction of raman line-shapes from congested cars spectra, " Opt. Express 14, 3622-3630 (2006).
10. Y. Liu, Y. J. Lee, and M. T. Cicerone, "Broadband cars spectral phase retrieval using a time-domain kramerskronig transform, " Opt. Lett. 34, 1363-1365 (2009).
11. H. A. Rinia, K. N. J. Burger, M. Bonn, and M. Müller, "Quantitative label-free imaging of lipid composition and packing of individual cellular lipid droplets using multiplex cars microscopy, " Biophys. J. 95, 4908-4914 (2008).
12. I. Rocha-Mendoza, W. Langbein, P. Watson, and P. Borri, "Differential coherent anti-stokes raman scattering microscopy with linearly-chirped femtosecond laser pulses, " Opt. Lett. 34, 2258-2260 (2009).
13. W. Langbein, I. Rocha-Mendoza, and P. Borri, "Single source coherent anti-stokes raman microspectroscopy using spectral focusing, " Appl. Phys. Lett. 95, 081109 (2009).
14. I. Rocha-Mendoza, P. Borri, and W. Langbein, "Quadruplex cars micro-spectroscopy, " J. Raman Spectr. 44, 255-261 (2013).
15. C. Di Napoli, F. Masia, I. Pope, C. Otto, W. Langbein, and P. Borri, "Chemically-specific dual/differential cars micro-spectroscopy of saturated and unsaturated lipid droplets, " J. Biophotonics 7, 68-76 (2014 (2012 online).
16. F. Masia, A. Glen, P. Stephens, P. Borri, and W. Langbein, "Quantitative chemical imaging and unsupervised analysis using hyperspectral coherent anti-stokes raman scattering microscopy, " Anal. Chem. 85, 10820-10828 (2013).
17. I. Pope, W. Langbein, P. Watson, and P. Borri, "Simultaneous hyperspectral differential-CARS, TPF and SHG microscopy with a single 5 fs ti:sa laser, " Opt. Express 21, 7096-7106 (2013).
18. T. Hellerer, A. M. Enejder, and A. Zumbusch, "Spectral focusing: High spectral resolution spectroscopy with broad-bandwidth laser pulses, " Appl. Phys. Lett. 85, 25-27 (2004).
19. I. Rocha-Mendoza, W. Langbein, and P. Borri, "Coherent anti-stokes raman microspectroscopy using spectral focusing with glass dispersion, " Appl. Phys. Lett. 93, 201103 (2008).
20. L. L. Listenberger and D. A. Brown, "Fluorescent detection of lipid droplets and associated proteins, " Curr. Protoc. Cell Biol. 24.2, 24.2.1-24.2.11 (2007).
21. L. Hodson, C. M. Skeaff, and B. A. Fielding, "Fatty acid composition of adipose tissue and blood in humans and its use as a biomarker of dietary intake, " Progress in Lipid Research 47, 348-380 (2008).
22. D. Gachet, F. Billard, N. Sandeau, and H. Rigneault, "Coherent anti-Stokes Raman scattering (CARS) microscopy imaging at interfaces: evidence of interference effects" Opt. Express 15, 10408-10420 (2007).
23. A. M. Barlow, K. Popov, M. Andreana, D. J. Moffatt, A. Ridsdale, A. D. Slepkov, J. L. Harden, L. Ramunno, and A. Stolow, "Spatial-spectral coupling in coherent anti-Stokes Raman scattering microscopy" Opt. Express 21, 15298-15307 (2013).