In vivo kinetic analysis of the penicillin biosynthesis pathway using PAA stimulus response experiments

Metabolic Engineering

Volumn 32, Issue , 2015, Pages 155-173

  Deshmukh, Amit T ^a,b   Verheijen, Peter J T ^a   Maleki Seifar, Reza ^a   Heijnen, Joseph J ^a   van Gulik, Walter M ^a  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

^b DSM BIOTECHNOLOGY CENTER   (Netherlands)

Author keywords

Acyl CoA:Isopenicillin N Acyltransferase; In vivo enzyme kinetics; Kinetic modeling; Penicillin biosynthesis pathway; Penicillium chrysogenum

Indexed keywords

BIOCHEMISTRY; BIOMOLECULES; BIOSYNTHESIS; CELL MEMBRANES; ENZYME ACTIVITY; ENZYME KINETICS; ENZYMES; KINETICS; METABOLITES;

ACYLTRANSFERASE; BIOSYNTHESIS PATHWAYS; IN-VIVO; KINETIC MODELING; PENICILLIUM CHRYSOGENUM;

DRUG PRODUCTS;

6 AMINOPENICILLANIC ACID; PENICILLIN DERIVATIVE; PENICILLIN G; PHENYLACETIC ACID; ACYL-COA-6-AMINOPENICILLANIC ACID ACYLTRANSFERASE; ACYLTRANSFERASE; CARBON; CULTURE MEDIUM; GLUCOSE; PENICILLANIC ACID; PENICILLIN BINDING PROTEIN; PHENYLACETIC ACID DERIVATIVE;

ARTICLE; CARBOXYLATION; CELL PH; DRUG SYNTHESIS; ENZYME KINETICS; LIMIT OF DETECTION; NEGATIVE FEEDBACK; NONHUMAN; PRECURSOR; PRIORITY JOURNAL; STIMULUS RESPONSE; TRANSPORT KINETICS; ALGORITHM; ANALOGS AND DERIVATIVES; BIOLOGICAL MODEL; BIOSYNTHESIS; CULTURE MEDIUM; FILTRATION; GENETICS; KINETICS; METABOLISM; PENICILLIUM CHRYSOGENUM;

ACYLTRANSFERASES; ALGORITHMS; CARBON; CULTURE MEDIA; FILTRATION; GLUCOSE; KINETICS; METABOLIC NETWORKS AND PATHWAYS; MODELS, BIOLOGICAL; PENICILLANIC ACID; PENICILLIN-BINDING PROTEINS; PENICILLINS; PENICILLIUM CHRYSOGENUM; PHENYLACETATES;

EID: 84945981218     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2015.09.018     Document Type: Article

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