Transcription of the pcbAB, pcbC and penDE genes of Penicillium chrysogenum AS-P-78 is repressed by glucose and the repression is not reversed by alkaline pHs

Microbiology

Volumn 145, Issue 2, 1999, Pages 317-324

  Gutiérrez, Santiago ^a,b   Marcos, Ana T ^b   Casqueiro, Javier ^b   Kosalková, Katarina ^a   Fernández, Francisco J ^b   Velasco, Javier ^a   Martín, Juan F ^a,b  

^a UNIVERSITY OF LEÓN   (Spain)

^b Institute of Biotechnology INBIOTEC Parque Científico de León   (Spain)

Author keywords

Carbon catabolite regulation; Penicillin genes; pH effect; Transcription

Indexed keywords

GLUCOSE; PENICILLIN G;

ANTIBIOTIC BIOSYNTHESIS; ARTICLE; FUNGAL GENETICS; GENE LOCUS; GENETIC TRANSCRIPTION; NONHUMAN; PENICILLIUM CHRYSOGENUM; PRIORITY JOURNAL; REPORTER GENE;

PENICILLIUM CHRYSOGENUM;

EID: 18144451613     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/13500872-145-2-317     Document Type: Article

References (38)

1. Alvarez, E., Cantoral, J. M., Barredo, J. L., Díez, B. & Martín, J. F. (1987). Purification to homegeneity and characterization of acyl coenzyme A :6-aminopenicillanic acid acyltransferase of Penicillium chrysogenum. Antimicrob Agents Chemother 31, 1675-1682.
2. Aharonowitz, Y., Cohen, G. & Martín, J. F. (1992). Penicillin and cephalosporin biosynthetic genes: structure, organization, regulation, and evolution. Annu Rev Microbiol 46, 461-495.
3. Arst, H. N., Jr & MacDonald, D. W. (1975). A gene cluster in Aspergillus nidulans with an internally located cis-acting regulatory region. Nature 254, 26-31.
4. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D., Smith, J. A., Seidman, J. G. & Struhl, K. (1987). Current Protocols in Molecular Biology. New York: Wiley.
5. Bailey, C. & Arst, H. N., Jr (1975). Carbon catabolite repression in Aspergillus nidulans. Eur J Biochem 51, 573-577.
6. Barredo, J. L., Alvarez, E., Cantoral, J. M., Díez, B. & Martín, J. f. (1988). Glucokinase-deficient mutant of Penicillium chrysogenum is derepressed in glucose cataholite regulation of both β-galactosidase and penicillin biosynthesis. Antimicrob Agents Chemother 32, 1061-1067.
7. Barredo, J. L., Cantoral, J. M., Alvarez, E., Díez, B. & Martín, J. F. (1989a). Cloning, sequence analysis and transcriptional study of the isopenicillin N synthase of Penicillium chrysogenum AS-P-78. Mol Gen Genet 216, 91-98.
8. Barredo, J. L., van Solingen, P., Díez, B., Alvarez, E., Cantoral, J. M., Kattevilder, A., Smaal, E. B., Groenen, M. A. M., Veenstra, A. E. & Martín, J. F. (1989b). Cloning and characterization of acyl-CoA : 6-APA acyltransferase gene of Penicillium chrysogenum. Gene 83, 291-300.
9. Brakhage, A. A., Browne, P. & Turner, G. (1992). Regulation of Aspergillus nidulans penicillin biosynthesis genes acv A and ipn A by glucose. J Bacteriol 174, 3789-3799.
10. Caddick, M. X., Brownlee, A. G. & Arst, H. N., Jr (1986). Regulation of gene expression by pH of the growth medium in Aspergillus nidulans. Mol Gen Genet 203, 346-353.
11. Carr, L. G., Skatrud, P. L., Scheetz, M. E., Queener, S. W. & Ingolia, T. D. (1986). Cloning and expression of isopenicillin N synthetase gene from Penicillium chrysogenum. Gene 48, 257-266.
12. Chater, K. & Bibb, M. (1997). Regulation of bacterial antibiotic production. In Products of Secondary Metabolism, pp. 57-106. Edited by H. Kleinkauf & H. von Döhren. Weinheim: VCH.
13. Chu, Y. W., Renno, D. &Saunders, G. (1997). Extracellular pH affects regulation of the pcbAB gene in Penicillium chrysogenum. Appl Microbiol Biotechnol 47, 250-254.
14. Díez, B., Gutiérrez, S., Barredo, J. L., van Solingen, P., van der Voort, L. H. M. & Martín, J. F. (1990). The cluster of penicillin biosynthetic genes. Identification and characterization of the pcbAB gene encoding the α-aminoadipyl-cysteinyl-valine synthetase and linkage to the pcbC and penDE genes. J Biol Chem 265, 16358-16365.
15. Dowzer, C. E. A. & Kelly, J. M. (1991). Analysis of the creA gene, a regulator of carbon catabolite repression in Aspergillus nidulans. Mol Cell Biol 11, 5701-5709.
16. Espeso, E. & Peñalva, M. A. (1992). Carbon catabolite repression can account for the temporal pattern of expression of a penicillin structural gene in Aspergillus nidulans. Mol Microbiol 6, 1457-1465.
17. Espeso, E. A., Tilburn, J., Arst, H. N. & Peñalva, M. A. (1993). pH regulation of a major determinant in expression of a fungal penicillin biosynthetic gene. EMBO J 12, 3941-3958.
18. Feng, B., Friedlin, E. & Marzluf, G. A. (1994). A reporter gene analysis of penicillin biosynthesis gene expression in Penicillium chrysogenum and its regulation by nitrogen and glucose catabolite repression. Appl Environ Microbiol 60, 4432-4439.
19. Fierro, F., Gutiérrez, S., Díez, B. & Martín, J. F. (1993). Resolution of four large chromosomes in penicillin-producing filamentous fungi: the penicillin gene cluster is located on chromosome II (9.6 Mb) in Penicillium notatum and chromosome I (10.4 Mb) in Penicillium chrysogenum. Mol Gen Genet 241, 573-579.
20. Fierro, F., Barredo, J. L., Díez, B., Gutiérrez, S., Fernández, F. J. & Martín, J. F. (1995). The penicillin gene cluster is amplified in tandem repeats linked by conserved hexanucleotide sequences. Proc Natl Acad Sci USA 92, 6200-6204.
21. Fierro, F., Montenegro, E., Gutiérrez, S. & Martín, J. F. (1996). Mutants blocked in penicillin biosynthesis show a deletion of the entire penicillin gene cluster at a specific site within a conserved hexanucleotide sequence. Appl Microbiol Biotechnol 43, 597-604.
22. Gutiérrez, S., Díez, B., Montenegro, E. & Martín, J. F. (1991). Characterization of the Cephalosporium acremonium pcbAB gene encoding α-aminoadipyl-cysteinyl-valine synthetase, a large multidomain peptide synthetase: linkage to the pcbC gene as a cluster of early cephalosporin-biosynthetic genes and evidence of multiple functional domains. J Bacteriol 173, 2354-2365.
23. Kolar, M., Holzmenn, K., Weber, G., Leitner, E. & Schwab, H. (1991). Molecular characterization and functional analysis in Aspergillus nidulans of the 5′ region of the Penicillium chrysogenum isopenicillin N synthetase gene. J Biotechnol 17, 67-80.
24. Kulmburg, P., Mathieu, M., Dowzer, C., Kelly, J. & Felenbok, B. (1993). Specific binding sites in the alcR and alcA promoters of the ethanol regulon for the CreA repressor mediating carbon catabolite repression in Aspergillus nidulans. Mol Microbiol 7, 847-857.
25. Martín, J. F. (1998). New aspects of genes and enzymes for β-lactam antibiotic biosynthesis. Appl Microbiol Biotechnol 50, 1-15.
26. Martín, J. F. & Demain, A. L. (1980). Control of antibiotic synthesis. Microbiol Rev 44, 230-251.
27. Martín, J. F. & Liras, P. (1989). Organization and expression of genes involved in the biosynthesis of antibiotics and other secondary metabolites. Annu Rev Microbiol 43, 173-206.
28. Martín, J. F., Ingolia, T. D. & Queener, S. W. (1991). Molecular genetics of penicillin and cephalosporin antibiotic biosynthesis. In Molecular Industrial Mycology: Systems and Applications for Filamentous Fungi, pp. 149-196. Edited by S. A. Leong & R. M. Berka. New York: Marcel Dekker.
29. Miller, J. (1972). Experiments in Molecular Genetics: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
30. Pérez-Esteban, B., Orejas, M., Gómez-Pardo, E. & Peñalva, M. A. (1993). Molecular characterization of a fungal secondary metabolism promoter: transcription of the Aspergillus nidulans isopenicillin N synthetase gene is modulated by upstream negative elements. Mol Microbiol 9, 881-895.
31. Revilla, G., López-Nieto, M. J., Luengo, J. M. & Martín, J. F. (1984). Carbon catabolite repression of penicillin biosynthesis by Penicillium chrysogenum. J Antibiot 37, 781-789.
32. Revilla, G., Ramos, F. R., López-Nieto, M. J., Alvarez, E. & Martín, J. F. (1986). Glucose represses formation of δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine and isopenicillin N synthase but not penicillin acyltransferase in Penicillium chrysogenum. J Bacteriol 168, 947-952.
33. Shah, A. J., Tilburn, J., Adlard, M. W. & Arst, H. N., Jr (1991). pH regulation of penicillin production in Aspergillus nidulans. FEMS Microbiol Lett 77, 209-212.
34. Smith, D. J., Burnham, M. K. R., Bull, J. H. & 7 other authors (1990). β-Lactam antibiotic biosynthetic genes have been conserved in clusters in prokaryotes and eukaryotes. EMBO J 9, 741-747.
35. Suárez, T. & Peñalva, M. A. (1996). Characterization of a Penicilliurn chrysogenum gene encoding a PacC transcription factor and its binding sites in the divergent pcbAB-pcbC promoter of the penicillin biosynthetic cluster. Mol Microbiol 20, 529-540.
36. Tilburn, J., Sarkar, S., Widdick, D. A., Espeso, E. A., Orejas, M., Mungroo, J., Peñalva, M. A. & Arst, H. N., Jr (1995). The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid- and alkaline-expressed genes by ambient pH. EMBO J 14, 779-790.
37. Tobin, M. B., Fleming, M. D., Skatrud, P. L. & Miller, J. R. (1990). Molecular characterization of the acyl-coenzyme A: isopenicillin N acyltransferase gene (penDE) from Penicillium chrysogenum and Aspergillus nidulans and activity of recombinant enzyme in Escherichia coli. J Bacteriol 172, 5908-5914.
38. Zhang, J.-Y. & Demain, A. L. (1989). Phosphate regulation of ACV synthetase and cephalosporin biosynthesis in Streptomyces clavuligerus. FEMS Microbiol Lett 57, 145-150.