Cytosolic NADPH metabolism in penicillin-G producing and non-producing chemostat cultures of Penicillium chrysogenum

Metabolic Engineering

Volumn 9, Issue 1, 2007, Pages 112-123

  Kleijn, Roelco J ^a   Liu, Feng ^a   van Winden, Wouter A ^a   van Gulik, Walter M ^a   Ras, Cor ^a   Heijnen, Joseph J ^a  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

13C tracer; Flux analysis; NADPH; Penicillium chrysogenum; Pentose phosphate pathway

Indexed keywords

ANTIBIOTICS; FUNGI; METABOLISM; OXIDATION; STOICHIOMETRY; SYNTHESIS (CHEMICAL);

CHEMOSTAT CULTURES; CYTOSOLIC NADPH; FLUX ANALYSIS; PENICILLIN-G; PENTOSE PHOSPHATE PATHWAY;

CELL CULTURE;

GLUCONIC ACID; PENICILLIN DERIVATIVE; PENICILLIN G; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; CHEMOSTAT; COMPARATIVE STUDY; CYTOSOL; FUNGUS CULTURE; METABOLISM; NONHUMAN; OXIDATION; PENICILLIUM CHRYSOGENUM; PENTOSE PHOSPHATE CYCLE; PRIORITY JOURNAL; STOICHIOMETRY; SYNTHESIS; TECHNIQUE;

ANTI-BACTERIAL AGENTS; CYTOSOL; NADP; PENICILLIN G; PENICILLIUM CHRYSOGENUM; PENTOSE PHOSPHATE PATHWAY;

PENICILLIUM CHRYSOGENUM;

EID: 33845638400     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2006.08.004     Document Type: Article

References (25)

1. Blank L.M., and Sauer U. TCA cycle activity in Saccharomyces cerevisiae is a function of the environmentally determined specific growth and glucose uptake rates. Microbiology 150 (2004) 1085-1093
2. Christensen B., Thykaer J., and Nielsen J. Metabolic characterization of high- and low-yielding strains of Penicillium chrysogenum. Appl. Microbiol. Biotechnol. 54 2 (2000) 212-217
3. Christensen B., Gombert A.K., and Nielsen J. Analysis of flux estimates based on C-13 labelling experiments. Eur. J. Biochem. 269 11 (2002) 2795-2800
4. Evers M.E., Trip H., van den Berg M.A., Bovenberg R.A.L., and Driessen A.J.M. Compartmentalization and transport in beta-lactam antibiotics biosynthesis. In: Brakhage A.A. (Ed). Molecular Biotechnology of Fungal Beta-lactam Antibiotics and Related Peptide Synthetases Vol. 88 (2004), Springer, Berlin, Heidelberg 111-135
5. Gombert A.K., dos Santos M.M., Christensen B., and Nielsen J. Network identification and flux quantification in the central metabolism of Saccharomyces cerevisiae under different conditions of glucose repression. J. Bacteriol. 183 4 (2001) 1441-1451
6. Gunnarsson N., Eliasson A., and Nielsen J. Control of fluxes towards antibiotics and the role of primary metabolism in production of antibiotics. In: Brakhage A.A. (Ed). Molecular Biotechnology of Fungal Beta-lactam Antibiotics and Related Peptide Synthetases Vol. 88 (2004), Springer, Berlin, Heidelberg 137-178
7. Harris D.M., Diderich J.A., van der Krogt Z.A., Luttik M.A.H., Raamsdonk L.M., Bovenberg R.A.L., van Gulik W.M., van Dijken J.P., and Pronk J.T. Enzymic analysis of NADPH metabolism in [beta]-lactam-producing Penicillium chrysogenum: presence of a mitochondrial NADPH dehydrogenase. Metab. Eng. 8 2 (2006) 91-101
8. Henriksen C.M., Christensen L.H., Nielsen J., and Villadsen J. Growth energetics and metabolic fluxes in continuous cultures of Penicillium chrysogenum. J. Biotechnol. 45 2 (1996) 149-164
9. Jorgensen H., Nielsen J., Villadsen J., and Mollgaard H. Metabolic flux distributions in Penicillium chrysogenum during fed-batch cultivations. Biotechnol. Bioeng. 46 2 (1995) 117-131
10. Kiefer P., Heinzle E., Zelder O., and Wittmann C. Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose, or fructose. Appl. Environ. Microbiol. 70 1 (2004) 229-239
11. Kleijn R.J., van Winden W.A., Ras C., van Gulik W.M., Schipper D., and Heijnen J.J. 13C-Labeled gluconate tracing as a direct and accurate method for determining the pentose phosphate pathway split ratio in Penicillium chrysogenum. Appl. Environ. Microbiol. 72 7 (2006) 4743-4754
12. Lange H.C., Eman M., van Zuijlen G., Visser D., van Dam J.C., Frank J., de Mattos M.J., and Heijnen J.J. Improved rapid sampling for in vivo kinetics of intracellular metabolites in Saccharomyces cerevisiae. Biotechnol. Bioeng. 75 4 (2001) 406-415
13. Mashego M.R., van Gulik W.M., Vinke J.L., and Heijnen J.J. Critical evaluation of sampling techniques for residual glucose determination in carbon-limited chemostat culture of Saccharomyces cerevisiae. Biotechnol. Bioeng. 83 4 (2003) 395-399
14. Nasution U., van Gulik W.M., Kleijn R.J., van Winden W.A., Proell A., and Heijnen J.J. Measurement of intracellular metabolites of primary metabolism and adenine nucleotides in chemostat cultivated Penicillium chrysogenum. Biotechnol. Bioeng. 94 1 (2006) 159-166
15. Ostergaard S., Theilgaard H.B.A., and Nielsen J. Identification and purification of O-acetyl-l-serine sulphhydrylase in Penicillium chrysogenum. Appl. Microbiol. Biotechnol. 50 6 (1998) 663-668
16. Sawyer D.T., and Bagger J.B. The lactone acid salt quilibria for d-glucono-delta-lactone and the hydrolysis kinetics for this lactone. J. Am. Chem. Soc. 81 20 (1959) 5302-5306
17. Sonderegger M., Jeppsson M., Hahn-Hagerdal B., and Sauer U. Molecular basis for anaerobic growth of Saccharomyces cerevisiae on xylose, investigated by global gene expression and metabolic flux analysis. Appl. Environ. Microbiol. 70 4 (2004) 2307-2317
18. Thykaer J., and Nielsen J. Metabolic engineering of beta-lactam production. Metab. Eng. 5 1 (2003) 56-69
19. van Gulik W.M., de Laat W.T., Vinke J.L., and Heijnen J.J. Application of metabolic flux analysis for the identification of metabolic bottlenecks in the biosynthesis of penicillin-G. Biotechnol. Bioeng. 68 6 (2000) 602-618
20. van Gulik W.M., Antoniewicz M.R., deLaat W.T., Vinke J.L., and Heijnen J.J. Energetics of growth and penicillin production in a high-producing strain of Penicillium chrysogenum. Biotechnol. Bioeng. 72 2 (2001) 185-193
21. van Winden W.A., Wittmann C., Heinzle E., and Heijnen J.J. Correcting mass isotopomer distributions for naturally occurring isotopes. Biotechnol. Bioeng. 80 4 (2002) 477-479
22. van Winden W.A., van Gulik W.M., Schipper D., Verheijen P.J.T., Krabben P., Vinke J.L., and Heijnen J.J. Metabolic flux and metabolic network analysis of Penicillium chrysogenum using 2D [C-13, H-1] COSYNMR measurements and cumulative Bondomer simulation. Biotechnol. Bioeng. 83 1 (2003) 75-92
23. van Winden W.A., van Dam J.C., Ras C., Kleijn R.J., Vinke J.L., van Gulik W.M., and Heijnen J.J. Metabolic-flux analysis of Saccharomyces cerevisiae CEN.PK113-7D based on mass isotopomer measurements of 13C-labeled primary metabolites. FEMS Yeast Res. 5 6-7 (2005) 559-568
24. Vanderheijden R.T.J.M., Heijnen J.J., Hellinga C., Romein B., and Luyben K.C.A.M. Linear constraint relations in biochemical reaction systems .1. Classification of the calculability and the balanceability of conversion rates. Biotechnol. Bioeng. 43 1 (1994) 3-10
25. Zhu J., and Shimizu K. Effect of a single-gene knockout on the metabolic regulation in Escherichia coli for d-lactate production under microaerobic condition. Metab. Eng. 7 2 (2005) 104-115