Studies of isopenicillin N synthase enzymatic properties using a continuous spectrophotometric assay

FEBS Letters

Volumn 485, Issue 2-3, 2000, Pages 142-146

  Dubus, Alain ^a   Sami, Malkit ^b   Brown, Toby J N ^b   Schofield, Christopher J ^b   Baldwin, Jack E ^b   Frère, Jean Marie ^a  

^a UNIVERSITY OF LIÈGE   (Belgium)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

ACV, (L aminoadipoyl) L cysteinyl D valine; DAOCS, deacetoxycephalosporin C synthase; DTT, dithiothreitol; Enzyme assay; IPN, isopenicillin N; IPNS, isopenicillin N synthase; Isopenicillin N synthase; Oxidative damage; Oyxgenase; ROS, reactive oxygen species; TCEP, tris(carboxyethyl)phosphine; Lactam biosynthesis

Indexed keywords

ANTIOXIDANT; IRON COMPLEX; ISOPENICILLIN N SYNTHETASE; PENICILLIN DERIVATIVE; REACTIVE OXYGEN METABOLITE; REDUCING AGENT; ADICILLIN; ASCORBIC ACID; DITHIOTHREITOL; ENZYME ACTIVATOR; IRON; OXIDOREDUCTASE;

ARTICLE; CATALYSIS; CELL NUCLEUS; ENZYME ANALYSIS; ENZYME INACTIVATION; ENZYME MECHANISM; IN VITRO STUDY; IRON DEPLETION; NEGATIVE FEEDBACK; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; SPECTROPHOTOMETRY; STEADY STATE; ASPERGILLUS NIDULANS; ENZYMOLOGY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KINETICS; METABOLISM; ULTRAVIOLET SPECTROPHOTOMETRY;

ANTIOXIDANTS; ASCORBIC ACID; ASPERGILLUS NIDULANS; CATALYSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DITHIOTHREITOL; ENZYME REACTIVATORS; IRON; KINETICS; OXIDOREDUCTASES; PENICILLINS; REDUCING AGENTS; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 0034711328     PISSN: 00145793     EISSN: 18733468     Source Type: Journal    
DOI: 10.1016/S0014-5793(00)02221-3     Document Type: Article

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