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Volumn 4, Issue , 2014, Pages

Comprehensive profiling analysis of actively resorbing osteoclasts identifies critical signaling pathways regulated by bone substrate

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; CELL DIFFERENTIATION; CELL PROLIFERATION; CYTOLOGY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; METABOLISM; MOUSE; OSTEOCLAST; OSTEOLYSIS; PHYSIOLOGY;

EID: 84942179751     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep07595     Document Type: Article
Times cited : (20)

References (49)
  • 1
    • 0034009870 scopus 로고    scopus 로고
    • Pathogenesis of bone erosions in rheumatoid arthritis
    • Goldring, S. R. & Gravallese, E. M. Pathogenesis of bone erosions in rheumatoid arthritis. Curr Opin Rheumatol 12, 195-199 (2000).
    • (2000) Curr Opin Rheumatol , vol.12 , pp. 195-199
    • Goldring, S.R.1    Gravallese, E.M.2
  • 2
    • 0034284970 scopus 로고    scopus 로고
    • Bone resorption by osteoclasts
    • Teitelbaum, S. L. Bone resorption by osteoclasts. Science 289, 1504-1508 (2000).
    • (2000) Science , vol.289 , pp. 1504-1508
    • Teitelbaum, S.L.1
  • 3
  • 4
    • 0037673945 scopus 로고    scopus 로고
    • Osteoclast differentiation and activation
    • Boyle, W. J., Simonet, W. S. & Lacey, D. L. Osteoclast differentiation and activation. Nature 423, 337-342 (2003).
    • (2003) Nature , vol.423 , pp. 337-342
    • Boyle, W.J.1    Simonet, W.S.2    Lacey, D.L.3
  • 5
    • 0043267732 scopus 로고    scopus 로고
    • Genetic regulation of osteoclast development and function
    • Teitelbaum, S. L. & Ross, F. P. Genetic regulation of osteoclast development and function. Nat Rev Genet 4, 638-649 (2003).
    • (2003) Nat Rev Genet , vol.4 , pp. 638-649
    • Teitelbaum, S.L.1    Ross, F.P.2
  • 6
    • 0037174944 scopus 로고    scopus 로고
    • Large scale gene expression analysis of osteoclastogenesis in vitro and elucidation of NFAT2 as a key regulator
    • Ishida, N. et al. Large scale gene expression analysis of osteoclastogenesis in vitro and elucidation of NFAT2 as a key regulator. J Biol Chem 277, 41147-41156 (2002).
    • (2002) J Biol Chem , vol.277 , pp. 41147-41156
    • Ishida, N.1
  • 7
    • 18744366041 scopus 로고    scopus 로고
    • Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts
    • Takayanagi, H. et al. Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev Cell 3, 889-901 (2002).
    • (2002) Dev Cell , vol.3 , pp. 889-901
    • Takayanagi, H.1
  • 8
    • 80053137241 scopus 로고    scopus 로고
    • Bonematrix regulates osteoclast differentiation and annexin A8 gene expression
    • Crotti, T. N. et al. Bonematrix regulates osteoclast differentiation and annexin A8 gene expression. J Cell Physiol 226, 3413-3421 (2011).
    • (2011) J Cell Physiol , vol.226 , pp. 3413-3421
    • Crotti, T.N.1
  • 9
    • 84948468494 scopus 로고    scopus 로고
    • Annexin VIII Is a Critical Bone Matrix-dependent Osteoclast Gene Transcriptionally Regulated by RANKL and NFATc1
    • Hawaii, USA. Published by AMER SOC BONE & MINERAL RES, 2025 M ST, NW, Ste 800, Washington, DC 20036-3309 USA, September 19
    • McHugh, K. et al. Annexin VIII Is a Critical Bone Matrix-dependent Osteoclast Gene Transcriptionally Regulated by RANKL and NFATc1. Paper presented at: American Society for Bone and Mineral Research 29th Annual Meeting, Honolulu, Hawaii, USA. Published by AMER SOC BONE & MINERAL RES, 2025 M ST, NW, Ste 800, Washington, DC 20036-3309 USA, DOI: 10.1002/ jbmr.5650221409, 2007, September 19.
    • (2007) American Society for Bone and Mineral Research 29th Annual Meeting, Honolulu
    • McHugh, K.1
  • 10
    • 38449095532 scopus 로고    scopus 로고
    • Role of cell-matrix interactions in osteoclast differentiation
    • McHugh, K. P. et al. Role of cell-matrix interactions in osteoclast differentiation. Adv Exp Med Biol 602, 107-111 (2007).
    • (2007) Adv Exp Med Biol , vol.602 , pp. 107-111
    • McHugh, K.P.1
  • 11
    • 75749140068 scopus 로고    scopus 로고
    • The role of cell-substrate interaction in regulating osteoclast activation: Potential implications in targeting bone loss in rheumatoid arthritis
    • McHugh, K. P. et al. The role of cell-substrate interaction in regulating osteoclast activation: potential implications in targeting bone loss in rheumatoid arthritis. Ann Rheum Dis 69 Suppl 1, i83-85 (2010).
    • (2010) Ann Rheum Dis , vol.69 , pp. 83-85
    • McHugh, K.P.1
  • 12
    • 0033621890 scopus 로고    scopus 로고
    • Mice lacking beta3 integrins are osteosclerotic because of dysfunctional osteoclasts
    • McHugh, K. P. et al. Mice lacking beta3 integrins are osteosclerotic because of dysfunctional osteoclasts. J Clin Invest 105, 433-440 (2000).
    • (2000) J Clin Invest , vol.105 , pp. 433-440
    • McHugh, K.P.1
  • 13
    • 28544443670 scopus 로고    scopus 로고
    • Alphavbeta3 and macrophage colony-stimulating factor: Partners in osteoclast biology
    • Ross, F. P. & Teitelbaum, S. L. alphavbeta3 and macrophage colony-stimulating factor: partners in osteoclast biology. Immunol Rev 208, 88-105 (2005).
    • (2005) Immunol Rev , vol.208 , pp. 88-105
    • Ross, F.P.1    Teitelbaum, S.L.2
  • 14
    • 28144436381 scopus 로고    scopus 로고
    • Critical role of beta3 integrin in experimental postmenopausal osteoporosis
    • Zhao, H. et al. Critical role of beta3 integrin in experimental postmenopausal osteoporosis. J Bone Miner Res 20, 2116-2123 (2005).
    • (2005) J Bone Miner Res , vol.20 , pp. 2116-2123
    • Zhao, H.1
  • 15
    • 1542393588 scopus 로고    scopus 로고
    • Platelet and osteoclast beta3 integrins are critical for bone metastasis
    • Bakewell, S. J. et al. Platelet and osteoclast beta3 integrins are critical for bone metastasis. Proc Natl Acad Sci U S A 100, 14205-14210 (2003).
    • (2003) Proc Natl Acad Sci U S A , vol.100 , pp. 14205-14210
    • Bakewell, S.J.1
  • 16
    • 9444242129 scopus 로고    scopus 로고
    • Apatite-mediated actin dynamics in resorbing osteoclasts
    • Saltel, F., Destaing, O., Bard, F., Eichert, D. & Jurdic, P. Apatite-mediated actin dynamics in resorbing osteoclasts. Mol Biol Cell 15, 5231-5241 (2004).
    • (2004) Mol Biol Cell , vol.15 , pp. 5231-5241
    • Saltel, F.1    Destaing, O.2    Bard, F.3    Eichert, D.4    Jurdic, P.5
  • 17
    • 0035050392 scopus 로고    scopus 로고
    • Cloning and characterization of the murine beta(3) integrin gene promoter: Identification of an interleukin-4 responsive element and regulation by STAT-6
    • McHugh, K. P., Kitazawa, S., Teitelbaum, S. L. & Ross, F. P. Cloning and characterization of the murine beta(3) integrin gene promoter: identification of an interleukin-4 responsive element and regulation by STAT-6. J Cell Biochem 81, 320-332 (2001).
    • (2001) J Cell Biochem , vol.81 , pp. 320-332
    • McHugh, K.P.1    Kitazawa, S.2    Teitelbaum, S.L.3    Ross, F.P.4
  • 18
    • 84863991650 scopus 로고    scopus 로고
    • C-Src links a RANK/alphavbeta3 integrin complex to the osteoclast cytoskeleton
    • Izawa, T. et al. c-Src links a RANK/alphavbeta3 integrin complex to the osteoclast cytoskeleton. Mol Cell Biol 32, 2943-2953 (2012).
    • (2012) Mol Cell Biol , vol.32 , pp. 2943-2953
    • Izawa, T.1
  • 19
    • 77951625288 scopus 로고    scopus 로고
    • Dissection of platelet and myeloid cell defects by conditional targeting of the beta3-integrin subunit
    • Morgan, E. A. et al. Dissection of platelet and myeloid cell defects by conditional targeting of the beta3-integrin subunit. FASEB J 24, 1117-1127 (2010).
    • (2010) FASEB J , vol.24 , pp. 1117-1127
    • Morgan, E.A.1
  • 20
    • 35948935128 scopus 로고    scopus 로고
    • Involvement of alpha(v)beta3 integrins in osteoclast function
    • Nakamura, I., Duong le, T., Rodan, S. B. & Rodan, G. A. Involvement of alpha(v)beta3 integrins in osteoclast function. J Bone Miner Metab 25, 337-344 (2007).
    • (2007) J Bone Miner Metab , vol.25 , pp. 337-344
    • Nakamura, I.1    Duong le, T.2    Rodan, S.B.3    Rodan, G.A.4
  • 21
    • 0035931748 scopus 로고    scopus 로고
    • Convergence of alpha(v)beta(3) integrin- and macrophage colony stimulating factor-mediated signals on phospholipase Cgamma in prefusion osteoclasts
    • Nakamura, I., Lipfert, L., Rodan, G. A. & Le, T. D. Convergence of alpha(v)beta(3) integrin- and macrophage colony stimulating factor-mediated signals on phospholipase Cgamma in prefusion osteoclasts. J Cell Biol 152, 361-373 (2001).
    • (2001) J Cell Biol , vol.152 , pp. 361-373
    • Nakamura, I.1    Lipfert, L.2    Rodan, G.A.3    Le, T.D.4
  • 22
    • 14844317695 scopus 로고    scopus 로고
    • Unoccupied alpha(v)beta3 integrin regulates osteoclast apoptosis by transmitting a positive death signal
    • Zhao, H., Ross, F. P. & Teitelbaum, S. L. Unoccupied alpha(v)beta3 integrin regulates osteoclast apoptosis by transmitting a positive death signal. Mol Endocrinol 19, 771-780 (2005).
    • (2005) Mol Endocrinol , vol.19 , pp. 771-780
    • Zhao, H.1    Ross, F.P.2    Teitelbaum, S.L.3
  • 23
    • 63649143900 scopus 로고    scopus 로고
    • Sphingosine-1-phosphate mobilizes osteoclast precursors and regulates bone homeostasis
    • Ishii, M. et al. Sphingosine-1-phosphate mobilizes osteoclast precursors and regulates bone homeostasis. Nature 458, 524-528 (2009).
    • (2009) Nature , vol.458 , pp. 524-528
    • Ishii, M.1
  • 24
    • 78650413566 scopus 로고    scopus 로고
    • Chemorepulsion by blood S1P regulates osteoclast precursor mobilization and bone remodeling in vivo
    • Ishii, M., Kikuta, J., Shimazu, Y., Meier-Schellersheim, M. & Germain, R. N. Chemorepulsion by blood S1P regulates osteoclast precursor mobilization and bone remodeling in vivo. J Exp Med 207, 2793-2798 (2010).
    • (2010) J Exp Med , vol.207 , pp. 2793-2798
    • Ishii, M.1    Kikuta, J.2    Shimazu, Y.3    Meier-Schellersheim, M.4    Germain, R.N.5
  • 25
    • 79960699262 scopus 로고    scopus 로고
    • The role of sphingosine 1-phosphate in migration of osteoclast precursors; An application of intravital two-photon microscopy
    • Ishii, T., Shimazu, Y., Nishiyama, I., Kikuta, J. & Ishii, M. The role of sphingosine 1-phosphate in migration of osteoclast precursors; an application of intravital two-photon microscopy. Mol Cells 31, 399-403 (2011).
    • (2011) Mol Cells , vol.31 , pp. 399-403
    • Ishii, T.1    Shimazu, Y.2    Nishiyama, I.3    Kikuta, J.4    Ishii, M.5
  • 26
    • 84876864069 scopus 로고    scopus 로고
    • Sphingosine-1-phosphate-mediated osteoclast precursor monocyte migration is a critical point of control in antibone-resorptive action of active Vitamin D
    • Kikuta, J. et al. Sphingosine-1-phosphate-mediated osteoclast precursor monocyte migration is a critical point of control in antibone-resorptive action of active vitamin D. Proc Natl Acad Sci U S A 110, 7009-7013 (2013).
    • (2013) Proc Natl Acad Sci U S A , vol.110 , pp. 7009-7013
    • Kikuta, J.1
  • 27
    • 58549115903 scopus 로고    scopus 로고
    • Regulation of bone formation by osteoclasts involves Wnt/BMP signaling and the chemokine sphingosine-1-phosphate
    • Pederson, L., Ruan, M., Westendorf, J. J., Khosla, S. & Oursler, M. J. Regulation of bone formation by osteoclasts involves Wnt/BMP signaling and the chemokine sphingosine-1-phosphate. Proc Natl Acad Sci U S A 105, 20764-20769 (2008).
    • (2008) Proc Natl Acad Sci U S A , vol.105 , pp. 20764-20769
    • Pederson, L.1    Ruan, M.2    Westendorf, J.J.3    Khosla, S.4    Oursler, M.J.5
  • 28
    • 33845714356 scopus 로고    scopus 로고
    • Sphingosine 1-phosphate as a regulator of osteoclast differentiation and osteoclast-osteoblast coupling
    • Ryu, J. et al. Sphingosine 1-phosphate as a regulator of osteoclast differentiation and osteoclast-osteoblast coupling. EMBO J 25, 5840-5851 (2006).
    • (2006) EMBO J , vol.25 , pp. 5840-5851
    • Ryu, J.1
  • 29
    • 84897954582 scopus 로고    scopus 로고
    • Molecular and physiological functions of sphingosine 1-phosphate transporters
    • Nishi, T., Kobayashi, N., Hisano, Y., Kawahara, A. & Yamaguchi, A. Molecular and physiological functions of sphingosine 1-phosphate transporters. Biochim Biophys Acta 1841, 759-765 (2014).
    • (2014) Biochim Biophys Acta , vol.1841 , pp. 759-765
    • Nishi, T.1    Kobayashi, N.2    Hisano, Y.3    Kawahara, A.4    Yamaguchi, A.5
  • 30
    • 77955412772 scopus 로고    scopus 로고
    • Discovery of novel sphingosine kinase-1 inhibitors. Part 2
    • Xiang, Y. et al. Discovery of novel sphingosine kinase-1 inhibitors. Part 2. Bioorg Med Chem Lett 20, 4550-4554 (2010).
    • (2010) Bioorg Med Chem Lett , vol.20 , pp. 4550-4554
    • Xiang, Y.1
  • 31
    • 55849088412 scopus 로고    scopus 로고
    • NFATc1 in mice represses osteoprotegerin during osteoclastogenesis and dissociates systemic osteopenia from inflammation in cherubism
    • Aliprantis, A. O. et al. NFATc1 in mice represses osteoprotegerin during osteoclastogenesis and dissociates systemic osteopenia from inflammation in cherubism. J Clin Invest 118, 3775-3789 (2008).
    • (2008) J Clin Invest , vol.118 , pp. 3775-3789
    • Aliprantis, A.O.1
  • 32
    • 84883685078 scopus 로고    scopus 로고
    • PU.1 target genes undergo Tet2-coupled demethylation and DNMT3b-mediated methylation in monocyte-to-osteoclast differentiation
    • de la Rica, L. et al. PU.1 target genes undergo Tet2-coupled demethylation and DNMT3b-mediated methylation in monocyte-to-osteoclast differentiation. Genome Biol 14, R99 (2013).
    • (2013) Genome Biol , vol.14 , pp. R99
    • De La Rica, L.1
  • 33
    • 77957664009 scopus 로고    scopus 로고
    • MiRNA-based mechanism for the commitment of multipotent progenitors to a single cellular fate
    • Mann, M., Barad, O., Agami, R., Geiger, B. & Hornstein, E. miRNA-based mechanism for the commitment of multipotent progenitors to a single cellular fate. Proc Natl Acad Sci U S A 107, 15804-15809 (2010).
    • (2010) Proc Natl Acad Sci U S A , vol.107 , pp. 15804-15809
    • Mann, M.1    Barad, O.2    Agami, R.3    Geiger, B.4    Hornstein, E.5
  • 34
    • 77951074209 scopus 로고    scopus 로고
    • The Blimp1-Bcl6 axis is critical to regulate osteoclast differentiation and bone homeostasis
    • Miyauchi, Y. et al. The Blimp1-Bcl6 axis is critical to regulate osteoclast differentiation and bone homeostasis. J Exp Med 207, 751-762 (2010).
    • (2010) J Exp Med , vol.207 , pp. 751-762
    • Miyauchi, Y.1
  • 36
    • 77955845729 scopus 로고    scopus 로고
    • RANKL-RANK signaling regulates expression of xenotropic and polytropic virus receptor (XPR1) in osteoclasts
    • Sharma, P., Patntirapong, S., Hann, S. & Hauschka, P. V. RANKL-RANK signaling regulates expression of xenotropic and polytropic virus receptor (XPR1) in osteoclasts. Biochem Biophys Res Commun 399, 129-132 (2010).
    • (2010) Biochem Biophys Res Commun , vol.399 , pp. 129-132
    • Sharma, P.1    Patntirapong, S.2    Hann, S.3    Hauschka, P.V.4
  • 38
    • 16544395502 scopus 로고    scopus 로고
    • Osteoclast differentiation by RANKL requires NF-kappaBmediated downregulation of cyclin-dependent kinase 6 (Cdk6)
    • Ogasawara, T. et al. Osteoclast differentiation by RANKL requires NF-kappaBmediated downregulation of cyclin-dependent kinase 6 (Cdk6). J Bone Miner Res 19, 1128-1136 (2004).
    • (2004) J Bone Miner Res , vol.19 , pp. 1128-1136
    • Ogasawara, T.1
  • 39
    • 34547448131 scopus 로고    scopus 로고
    • Receptor activator of nuclear factor kappa B ligand (RANKL) modulates the expression of genes involved in apoptosis and cell cycle in human osteoclasts
    • Rimondi, E., Zweyer, M., Ricci, E., Fadda, R. & Secchiero, P. Receptor activator of nuclear factor kappa B ligand (RANKL) modulates the expression of genes involved in apoptosis and cell cycle in human osteoclasts. Anat Rec (Hoboken) 290, 838-845 (2007).
    • (2007) Anat Rec (Hoboken) , vol.290 , pp. 838-845
    • Rimondi, E.1    Zweyer, M.2    Ricci, E.3    Fadda, R.4    Secchiero, P.5
  • 40
    • 16644392773 scopus 로고    scopus 로고
    • RANKL coordinates cell cycle withdrawal and differentiation in osteoclasts through the cyclin-dependent kinase inhibitors p27KIP1 and p21CIP1
    • Sankar, U., Patel, K., Rosol, T. J. & Ostrowski, M. C. RANKL coordinates cell cycle withdrawal and differentiation in osteoclasts through the cyclin-dependent kinase inhibitors p27KIP1 and p21CIP1. J Bone Miner Res 19, 1339-1348 (2004).
    • (2004) J Bone Miner Res , vol.19 , pp. 1339-1348
    • Sankar, U.1    Patel, K.2    Rosol, T.J.3    Ostrowski, M.C.4
  • 41
    • 34547207232 scopus 로고    scopus 로고
    • Suppression of osteoclastogenesis by N, N-dimethyl-D-erythrosphingosine: A sphingosine kinase inhibition-independent action
    • Kim, H. J. et al. Suppression of osteoclastogenesis by N, N-dimethyl-D-erythrosphingosine: a sphingosine kinase inhibition-independent action. Mol Pharmacol 72, 418-428 (2007).
    • (2007) Mol Pharmacol , vol.72 , pp. 418-428
    • Kim, H.J.1
  • 42
    • 77956894066 scopus 로고    scopus 로고
    • Genetic sphingosine kinase 1 deficiency significantly decreases synovial inflammation and joint erosions in murine TNF-alpha-induced arthritis
    • Baker, D. A., Barth, J., Chang, R., Obeid, L. M. & Gilkeson, G. S. Genetic sphingosine kinase 1 deficiency significantly decreases synovial inflammation and joint erosions in murine TNF-alpha-induced arthritis. J Immunol 185, 2570-2579 (2010).
    • (2010) J Immunol , vol.185 , pp. 2570-2579
    • Baker, D.A.1    Barth, J.2    Chang, R.3    Obeid, L.M.4    Gilkeson, G.S.5
  • 43
    • 77953923379 scopus 로고    scopus 로고
    • Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2
    • Alvarez, S. E. et al. Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2. Nature 465, 1084-1088 (2010).
    • (2010) Nature , vol.465 , pp. 1084-1088
    • Alvarez, S.E.1
  • 44
    • 0034523328 scopus 로고    scopus 로고
    • TNF-alpha induces osteoclastogenesis by direct stimulation of macrophages exposed to permissive levels of RANK ligand
    • Lam, J. T. S., Barker, J. E., Kanagawa, O., Ross, F. P. & Teitelbaum, S. L. TNF-alpha induces osteoclastogenesis by direct stimulation of macrophages exposed to permissive levels of RANK ligand. J Clin Invest. 106, 1481-1488 (2000).
    • (2000) J Clin Invest. , vol.106 , pp. 1481-1488
    • Lam, J.T.S.1    Barker, J.E.2    Kanagawa, O.3    Ross, F.P.4    Teitelbaum, S.L.5
  • 45
    • 78651036947 scopus 로고
    • Histochemical demonstration of acid phosphatases with naphthol AS-phosphates
    • Burstone, M. S. Histochemical demonstration of acid phosphatases with naphthol AS-phosphates. J Natl Cancer Inst 21, 523-539 (1958).
    • (1958) J Natl Cancer Inst , vol.21 , pp. 523-539
    • Burstone, M.S.1
  • 46
    • 27344435774 scopus 로고    scopus 로고
    • Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles
    • Subramanian, A. et al.Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci U S A 102, 15545-15550 (2005).
    • (2005) Proc Natl Acad Sci U S A , vol.102 , pp. 15545-15550
    • Subramanian, A.1
  • 47
    • 0033940801 scopus 로고    scopus 로고
    • Identification and characterization of the new osteoclast progenitor with macrophage phenotypes being able to differentiate into mature osteoclasts
    • Takeshita, S., Kaji, K. & Kudo, A. Identification and characterization of the new osteoclast progenitor with macrophage phenotypes being able to differentiate into mature osteoclasts. J Bone Miner Res 15, 1477-1488 (2000).
    • (2000) J Bone Miner Res , vol.15 , pp. 1477-1488
    • Takeshita, S.1    Kaji, K.2    Kudo, A.3
  • 48
    • 33645678219 scopus 로고    scopus 로고
    • Wear Debris Inhibition of Anti-Osteoclastogenic Signaling by Interleukin-6 and Interferon-{gamma}Mechanistic Insights and Implications for Periprosthetic Osteolysis
    • Rakshit, D. S. et al.Wear Debris Inhibition of Anti-Osteoclastogenic Signaling by Interleukin-6 and Interferon-{gamma}Mechanistic Insights and Implications for Periprosthetic Osteolysis. J Bone Joint Surg Am 88, 788-799 (2006).
    • (2006) J Bone Joint Surg Am , vol.88 , pp. 788-799
    • Rakshit, D.S.1
  • 49
    • 0035710746 scopus 로고    scopus 로고
    • Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method
    • Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402-408 (2001).
    • (2001) Methods , vol.25 , pp. 402-408
    • Livak, K.J.1    Schmittgen, T.D.2


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