Primary Epithelial Cell Models for Cystic Fibrosis Research

Methods in Molecular Biology

Volumn 742, Issue , 2011, Pages 285-310

  Randell, Scott H ^a   Fulcher, M Leslie ^a   O’Neal, Wanda ^a   Olsen, John C ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

adenovirus; differentiation; lentivirus; pathogenesis; physiology; Respiratory tract; therapy

Indexed keywords

DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; SMALL INTERFERING RNA; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ADENOVIRUS; ARTICLE; CELL DIFFERENTIATION; CELL LINE; CHEMISTRY; CRYOPRESERVATION; CULTURE MEDIUM; CULTURE TECHNIQUE; CYSTIC FIBROSIS; CYTOLOGY; EPITHELIUM CELL; GENE EXPRESSION; GENE SILENCING; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; HUMAN; METABOLISM; METHODOLOGY; PATHOLOGY; REPORTER GENE; RESPIRATORY TRACT MUCOSA; RETROVIRUS; TISSUE ENGINEERING;

ADENOVIRIDAE; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE; CRYOPRESERVATION; CULTURE MEDIA; CYSTIC FIBROSIS; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; EPITHELIAL CELLS; GENE EXPRESSION; GENE SILENCING; GENES, REPORTER; GENETIC VECTORS; HUMANS; INDICATORS AND REAGENTS; RESPIRATORY MUCOSA; RETROVIRIDAE; RNA, SMALL INTERFERING; TISSUE ENGINEERING; TRANSFECTION;

EID: 80052528051     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-120-8_18     Document Type: Chapter

References (26)

1. Lechner, J. F., Haugen, A., McLendon, I. A., and Pettis, E. W. (1982) Clonal growth of normal adult human bronchial epithelial cells in a serum-free medium. In Vitro 18, 633–642.
2. Fulcher, M. L., Gabriel, S., Burns, K. A., Yankaskas, J. R., and Randell, S. H. (2005) Well-differentiated human airway epithelial cell cultures. Methods Mol Med 107, 183–206.
3. Lechner, J. F., and LaVeck, M. A. (1985) A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J Tiss Cult Methods 9, 43–48.
4. Gray, T. E., Guzman, K., Davis, C. W., Abdullah, L. H., and Nettesheim, P. (1996) Mucociliary differentiation of serially passaged normal human tracheobronchial epithelial cells. Am J Respir Cell Mol Biol 14, 104–112.
5. Gazdar, A. F., and Minna, J. D. (1996) NCI series of cell lines: an historical perspective. J Cell Biochem Suppl 24, 1–11.
6. Boers, J. E., Ambergen, A. W., and Thunnissen, F. B. (1998) Number and proliferation of basal and parabasal cells in normal human airway epithelium. Am J Respir Crit Care Med 157, 2000–2006.
7. Stoner, G. D., Katoh, Y., Foidart, J. M., Myers, G. A., and Harris, C. C. (1980) Identification and culture of human bronchial epithelial cells. Methods Cell Biol 21A, 15–35.
8. Lundberg, A. S., Randell, S. H., Stewart, S. A., Elenbaas, B., Hartwell, K. A., Brooks, M. W., et al. (2002) Immortalization and transformation of primary human airway epithelial cells by gene transfer. Oncogene 21, 4577– 4586.
9. Zabner, J., Karp, P., Seiler, M., Phillips, S. L., Mitchell, C. J., Saavedra, M., et al. (2003) Development of cystic fibrosis and noncystic fibrosis airway cell lines. Am J Physiol 284, L844–L854.
10. Gruenert, D. C., Basbaum, C. B., Welsh, M. J., Li, M., Finkbeiner, W. E., and Nadel, J. A. (1988) Characterization of human tracheal epithelial cells transformed by an origindefective simian virus 40. Proc Natl Acad Sci USA 85, 5951–5955.
11. Masui, T., Lechner, J. F., Yoakum, G. H., Willey, J. C., and Harris, C. C. (1986) Growth and differentiation of normal and transformed human bronchial epithelial cells. J Cell Physiol Suppl 4, 73–81.
12. Ramirez, R. D., Sheridan, S., Girard, L., Sato, M., Kim, Y., Pollack, J., et al. (2004) Immortalization of human bronchial epithelial cells in the absence of viral oncoproteins. Cancer Res 64, 9027–9034.
13. Gruenert, D. C., Willems, M., Cassiman, J. J., and Frizzell, R. A. (2004) Established cell lines used in cystic fibrosis research. JCyst Fibros 3 Suppl 2, 191–196.
14. Fulcher, M. L., Gabriel, S. E., Olsen, J. C., Tatreau, J. R., Gentzsch, M., Livanos, E., et al. (2009) Novel human bronchial epithelial cell lines for cystic fibrosis research. Am J Physiol Lung Cell Mol Physiol 296, L82–L91.
15. Coyne, C. B., Kelly, M. M., Boucher, R. C., and Johnson, L. G. (2000) Enhanced epithelial gene transfer by modulation of tight junctions with sodium caprate. Am J Respir Cell Mol Biol 23, 602–609.
16. Wu, Q., Lu, Z., Verghese, M. W., and Randell, S. H. (2005) Airway epithelial cell tolerance to Pseudomonas aeruginosa. Respir Res 6, 26.
17. Luo, J., Deng, Z. L., Luo, X., Tang, N., Song, W. X., Chen, J., et al. (2007) A protocol for rapid generation of recombinant adenoviruses using the AdEasy system. Nat Protoc 2, 1236–1247.
18. Cockrell, A. S., and Kafri, T. (2007) Gene delivery by lentivirus vectors. Mol Biotechnol 36, 184–204.
19. Shin, K. J., Wall, E. A., Zavzavadjian, J. R., Santat, L. A., Liu, J., Hwang, J. I., et al. (2006) A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Proc Natl Acad Sci USA 103, 13759–13764.
20. Jones, L. C., Wonsetler, R. L., Olsen, J., Davis, W. C., Randell, S. H., Stutts, M., and O’Neal, W. K. (2008) Short hairpin RNA knockdown in well-differentiated human bronchial epithelial cells: A method for querying gene function relevant to cystic fibrosis. Pediatr Pulmonol Suppl 31, 284. [Abstract].
21. Randell, S. H., Walstad, L., Schwab, U. E., Grubb, B. R., and Yankaskas, J. R. (2001) Isolation and culture of airway epithelial cells from chronically infected human lungs. In Vitro Cell Dev Biol Anim 37, 480–489.
22. Dull, T., Zufferey, R., Kelly, M., Mandel, R.J., Nguyen, M., Trono, D., et al. (1998) A third-generation lentivirus vector with a conditional packaging system. JVirol72, 8463– 8471.
23. Johnson, L. G., Mewshaw, J. P., Ni, H., Friedmann, T., Boucher, R. C., and Olsen, J. C. (1998) Effect of host modification and age on airway epithelial gene transfer mediated by a murine leukemia virus-derived vector. JVirol72, 8861–8872.
24. Salmon, P., Oberholzer, J., Occhiodoro, T., Morel, P., Lou, J., and Trono, D. (2000) Reversible immortalization of human primary cells by lentivector-mediated transfer of specific genes. Mol Ther 2, 404–414.
25. Sanlioglu, S., Williams, C. M., Samavati, L., Butler, N. S., Wang, G., and McCray, P. B., Jr. et al. (2001) Lipopolysaccharide induces Rac1-dependent reactive oxygen species formation and coordinates tumor necrosis factor-alpha secretion through IKK regulation of NF-kappa B. J Biol Chem 276, 30188–30198.
26. Cullen, B. R. (2006) Enhancing and confirming the specificity of RNAi experiments. Nat Methods 3, 677–681.