Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Wang, Xianlong ^a   Zhou, Jinwei ^b   Cao, Chunwei ^a   Huang, Jiaojiao ^a,c   Hai, Tang ^a   Wang, Yanfang ^d   Zheng, Qiantao ^a,c   Zhang, Hongyong ^a,c   Qin, Guosong ^a   Miao, Xiangnan ^a   Wang, Hongmei ^a,c   Cao, Suizhong ^b   Zhou, Qi ^a,c   Zhao, Jianguo ^a,c  

^a INSTITUTE OF ZOOLOGY   (China)

^b SICHUAN AGRICULTURAL UNIVERSITY   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^d INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

[No Author keywords available]

Indexed keywords

GUIDE RNA; MESSENGER RNA; MICROPHTHALMIA ASSOCIATED TRANSCRIPTION FACTOR;

ALLELE; ANIMAL; BLASTOCYST; CRISPR CAS SYSTEM; FIBROBLAST; GENE INACTIVATION; GENE TARGETING; GENETICS; GENOTYPING TECHNIQUE; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; PIG; PROCEDURES; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SITE DIRECTED MUTAGENESIS; ZYGOTE;

ALLELES; ANIMALS; BASE SEQUENCE; BLASTOCYST; CRISPR-CAS SYSTEMS; FIBROBLASTS; GENE KNOCK-IN TECHNIQUES; GENE KNOCKOUT TECHNIQUES; GENE TARGETING; GENOTYPING TECHNIQUES; MICROPHTHALMIA-ASSOCIATED TRANSCRIPTION FACTOR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RNA, GUIDE; RNA, MESSENGER; SUS SCROFA; ZYGOTE;

EID: 84939815032     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep13348     Document Type: Article

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