Production of transgenic miniature pigs by pronuclear microinjection

Transgenic Research

Volumn 10, Issue 6, 2001, Pages 577-582

  Uchida, Masaki ^a   Shimatsu, Yoshiki ^a   Onoe, Kyuichiro ^b   Matsuyama, Noriko ^b   Niki, Rikio ^a   Ikeda, Joh E ^c   Imai, Hiroshi ^d  

^a SLA Research Inc   (Japan)

^b SLA Research Laboratories   (Japan)

^c TOKAI UNIVERSITY   (Japan)

^d KYOTO UNIVERSITY   (Japan)

Author keywords

Gene transfer; Microinjection; Miniature pig; Transgenic

Indexed keywords

ANIMALIA; SUS SCROFA;

DNA; NERVE PROTEIN; NUCLEAR PROTEIN;

AGAR GEL ELECTROPHORESIS; ANIMAL; ARTICLE; BIOLOGICAL MODEL; GENE TRANSFER; GENETICS; METABOLISM; MINIPIG; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; SWINE; TRANSGENE; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BLOTTING, SOUTHERN; DNA; ELECTROPHORESIS, AGAR GEL; GENE TRANSFER TECHNIQUES; MODELS, GENETIC; NERVE TISSUE PROTEINS; NUCLEAR PROTEINS; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; SWINE; SWINE, MINIATURE; TRANSGENES;

EID: 0035543870     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1013059917280     Document Type: Article

References (39)

1. Control of ovulation rate and fertilization in prepuberal gilts
2. The Göttingen minipig in pharmacology and toxicology
3. Factors in the success of transgenic pig programs
4. Induction of fertile estrus in prepuberal gilts by treatment with a combination of pregnant mare's serum gonadotropin and human chorionic gonadotropin
5. Transgenic science
6. The generation of transgenic pigs as potential organ donors for humans
7. Selection for ovulation rate in swine: Correlated response in litter size and weight
8. Of pigs and men and research: A review of applications and analogies of the Pig, sus scrofa, in human medical research
9. Comparison of prepubertal and postpubertal young sows as a source of one-cell embryos for microinjection
10. Breeding program and population standards of the Göttingen miniature swine
11. Effect of culture conditions, donor age, and injection site on in vitro development of DNA microinjected porcine zygotes
12. Production of transgenic rabbits, sheep and pigs by microinjection
13. Genetic engineering of mammalian embryos
14. Transgenic bioreactors
15. Effect of pronuclear DNA microinjection on the development of porcine ova in utero
16. Production of transgenic swine
17. Uses of the pig as a laboratory animal
18. Developments in transgenic techniques in pigs
19. In-vitro development of zygotes from superovulated prepubertal and mature gilts
20. Recovery, structure and viability of porcine zygotes and 2-cell stages subjected to cenrifugation
21. Integration, expression and germ-line transmission of growth-related genes in pigs
22. Effects of transferred ova per recipient and dual use of donors as recipients on production of transgenic swine
23. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
24. The structure and expression of neuron-specific enolase gene
25. Induction of superovulation and recovery of fertilized oocytes in prepubertal miniature pigs after treatment with PG600
26. Detection of specific sequences among DNA fragments separated by gel electrophoresis
27. The minipig in toxicology
28. Swine in biomedical research: Management and models
29. Comparative anatomy and physiology of the pig
30. Introduction of a porcine growth hormone fusion gene into transgenic pigs promotes growth
31. Transgenic animals as models for human disease
32. Development of porcine ova that were centrifuged to permit visualization of pronuclei and nuclei
33. Transgenic animals as new approaches in pharmacological studies
34. Effect of number of transferred microinjected embryos on pregnancy rate and litter size of pigs
35. Evaluation of systems for collection of porcine zygotes for DNA microinjection and transfer
36. In vitro development of zygotes from prepubertal gilts after microinjection of DNA
37. Investigations into the control of litter size in swine: I. Comparative studies on in vitro development of Meishan and Yorkshire preimplantation embryos