Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporter

Journal of Biotechnology

Volumn 189, Issue , 2014, Pages 1-8

  Zhang, Jian Hua ^a   Pandey, Mritunjay ^a   Kahler, John F ^a   Loshakov, Anna ^a   Harris, Benjamin ^a   Dagur, Pradeep K ^b   Mo, Yin Yuan ^c   Simonds, William F ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^c UNIVERSITY OF MISSISSIPPI SCHOOL OF MEDICINE   (United States)

Author keywords

CAS9; CRISPR; EGFP reporter; GRNA; Specificity

Indexed keywords

CAS9; CRISPR; EGFP REPORTER; GRNA; SPECIFICITY;

CRISPR ASSOCIATED PROTEIN; GUIDE RNA; DNA;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA END JOINING REPAIR; DNA MODIFICATION; DNA SEQUENCE; DOUBLE STRANDED DNA BREAK; FLOW CYTOMETRY; FLUORESCENCE; GENE TARGETING; GENETIC ENGINEERING; GENETIC TRANSFECTION; GENOMICS; NONHUMAN; ANIMAL; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENETICS; HIGH THROUGHPUT SEQUENCING; HUMAN;

ANIMALS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; DNA; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; RNA, GUIDE;

EID: 84908457209     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.08.033     Document Type: Article

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