The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3- galactosyltransferase gene in porcine embryonic fibroblasts

Xenotransplantation

Volumn 21, Issue 3, 2014, Pages 291-300

  Sato, Masahiro ^a   Miyoshi, Kazuchika ^a   Nagao, Yozo ^a   Nishi, Yohei ^a   Ohtsuka, Masato ^b   Nakamura, Shingo ^c   Sakurai, Takayuki ^d   Watanabe, Satoshi ^e  

^a KAGOSHIMA UNIVERSITY   (Japan)

^b TOKAI UNIVERSITY   (Japan)

^c NATIONAL DEFENSE MEDICAL COLLEGE   (Japan)

^d SHINSHU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^e NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

Author keywords

3 galactosyltransferase; biallelic knockout; CRISPR Cas9; porcine embryonic fibroblasts; somatic cell nuclear transfer; targeted toxin; 1

Indexed keywords

ALPHA 1,3 GALACTOSYLTRANSFERASE; EPITOPE; GUIDE RNA; NUCLEASE; PROTEIN CAS9; SAPORIN; TOXIN; UNCLASSIFIED DRUG; ALPHA-1,3-GALACTOSYLTRANSFERASE 1, PORCINE; GALACTOSYLTRANSFERASE; GENETIC MARKER;

ALLELE; ANIMAL CELL; ARTICLE; BLASTOCYST; CELL NUCLEUS TRANSPLANTATION; CELL SURVIVAL; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; EMBRYO; EMBRYO CELL; EMBRYONIC FIBROBLAST; EXPRESSION VECTOR; FIBROBLAST; FRAMESHIFT MUTATION; GENE; GENE SEQUENCE; GENETIC TRANSFECTION; GGTA1 GENE; HYPERACUTE GRAFT REJECTION; NONHUMAN; PRIORITY JOURNAL; SOMATIC CELL; SWINE; TECHNOLOGY; XENOTRANSPLANTATION; ANIMAL; CELL LINE; CRISPR CAS SYSTEM; EVALUATION STUDY; GENE INACTIVATION; GENETIC MARKER; GENETICS; MALE; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CELL LINE; CRISPR-CAS SYSTEMS; FIBROBLASTS; GALACTOSYLTRANSFERASES; GENE KNOCKOUT TECHNIQUES; GENETIC MARKERS; MALE; SWINE;

EID: 84901934624     PISSN: 0908665X     EISSN: 13993089     Source Type: Journal    
DOI: 10.1111/xen.12089     Document Type: Article

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