Targeted toxin-based selectable drug-free enrichment of mammalian cells with high transgene expression

Biology

Volumn 2, Issue 1, 2013, Pages 341-355

  Sato, Masahiro ^a   Akasaka, Eri ^b   Saitoh, Issei ^b   Ohtsuka, Masato ^c   Nakamura, Shingo ^d   Sakurai, Takayuki ^e   Watanabe, Satoshi ^f  

^a KAGOSHIMA UNIVERSITY   (Japan)

^b KAGOSHIMA UNIVERSITY   (Japan)

^c TOKAI UNIVERSITY   (Japan)

^d NATIONAL DEFENSE MEDICAL COLLEGE   (Japan)

^e SHINSHU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^f NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

Author keywords

Endo galactosidase C; High transgene expression; Porcine embryonic fibroblasts; Saporin; Targeted toxin; Gal epitope

Indexed keywords

EID: 84901376292     PISSN: None     EISSN: 20797737     Source Type: Journal    
DOI: 10.3390/biology2010341     Document Type: Article

References (21)

1. Vaughan, H.A.; Loveland, B.E.; Sandrin, M.S. Gal(α1-3)Gal is the major xenoepitope expressed on pig endothelial cells recognized by naturally occurring cytotoxic human antibodies. Transplantation 1994, 58, 879-882.
2. Cascalho, M.; Platt, J.L. The immunological barrier to xenotransplantation. Immunity 2001, 14, 437-446.
3. Galili, U. The α-gal epitope (Galα1-3Galβ1-4GlcNAc-R) in xenotransplantation. Biochimie 2001, 83, 557-563.
4. Cooper, D.K.C.; Gollackner, B.; Sachs, D.H. Will the pig solve the transplantation backlog? Ann. Rev. Med. 2002, 53, 133-147.
5. Cooper, D.K.C.; Koren, E.; Oriol, R. Oligosaccharides and discordant xenotransplantation. Immunol. Rev. 1994, 141, 31-58.
6. Ogawa, H.; Muramatsu, H.; Kobayashi, T.; Morozumi, K.; Yokoyama, I.; Kurosawa, N.; Nakao, A.; Muramatsu, T. Molecular cloning of endo-β-galactosidase C and its application in removing α-galactosyl xenoantigen from blood vessels in the pig. J. Biol. Chem. 2000, 275, 19368-19374.
7. Yazaki, S.; Iwamoto, M.; Onishi, A.; Miwa, Y.; Suzuki, S.; Fuchimoto, D.; Sembon, S.; Furusawa, T.; Hashimoto, M.; Oishi, T.; et al. Successful cross-breeding of cloned pigs expressing endo-beta-galactosidase C and human decay accelerating factor. Xenotransplantation 2009, 16, 511-521.
8. Akasaka, E.; Watanabe, S.; Himaki, T.; Ohtsuka, M.; Yoshida, M.; Miyoshi, K.; Sato, M. Enrichment of xenograft-competent genetically modified pig cells using a targeted toxin, isolectin BS-I-B4 conjugate. Xenotransplantation 2010, 17, 81-89.
9. Himaki, T.; Watanabe, S.; Chi, H.; Yoshida, M.; Miyoshi, K.; Sato, M. Production of genetically modified porcine blastocysts by somatic cell nuclear transfer: Preliminary results toward production of xenograft-competent miniature pigs. J. Reprod. Dev. 2010, 56, 630-638.
10. Stirpe, F.; Barbieri, L.; Battelli, M.G.; Soria, M.; Lappi, D.A. Ribosome-inactivating proteins from plants: present status and future prospects. Biotechnology 1992, 10, 405-412.
11. Fussenegger, M.; Mazur, X.; Bailey, J.E. pTRIDENT, a novel vector family for tricistronic gene expression in mammalian cells. Biotechnol. Bioeng. 1998, 57, 1-10.
12. Fussenegger, M.; Schlatter, S.; Dätwyler, D.; Mazur, X.; Bailey, J.E. Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells. Nat. Biotechnol. 1998, 16, 468-472.
13. Douin, V.; Bornes, S.; Creancier, L.; Rochaix, P.; Favre, G.; Prats, A.-C.; Couderc, B. Use and comparison of different internal ribosomal entry sites (IRES) in tricistronic retroviral vectors. BMC Biotechnol. 2004, 4, 16.
14. Sato, M.; Ohtsuka, M.; Miura, H.; Miyoshi, K.; Watanabe, S. Determination of the optimal concentration of several selective drugs useful for generating multi-transgenic porcine embryonic fibroblasts. Reprod. Dom. Anim. 2012, 47, 759-765.
15. Larsen, R.D.; Rivera-Marrero, C.A.; Ernst, L.K.; Cummings, R.D.; Lowe, J.B. Frameshift and nonsense mutations in a human genomic sequence homologous to a murine UDP-Gal:β-DGal(1,4)-D-GlcNAc α(1,3)-galactosyltransferase cDNA. J. Biol. Chem. 1990, 265, 7055-7061.
16. Watanabe, S.; Misawa, M.; Matsuzaki, T.; Sakurai, T.; Muramatsu, T.; Yokomine, T.; Sato, M. Production and characterization of transgenic mice systemically expressing Endo-β-galactosidase C. Glycobiology 2008, 18, 9-19.
17. Watanabe, S.; Misawa, M.; Matsuzaki, T.; Sakurai, T.; Muramatsu, T.; Sato, M. A novel glycosylation signal regulates transforming growth factor β receptors as evidenced by endo-β-galactosidase C expression in rodent cells. Glycobiology 2011, 21, 482-492.
18. Sato, M.; Chi, H.; Miyoshi, K. Targeted toxin as a useful reagent for enrichment of α-Gal epitope-negative cells used for somatic cell nuclear transfer in pigs. In InTech Open Book Chapter: Xenotransplantation; Miyagawa, S., Ed.; InTech: Rijeka, Croatia, 2012.
19. Niwa, H.; Yamamura, K.; Miyazaki, J. Efficient selection for high-expression transformants with a novel eukaryotic vector. Gene 1991, 108, 193-200.
20. Sato, M.; Ishikawa, A.; Kimura, M. Direct injection of foreign DNA into mouse testis as a possible in vivo gene transfer system via epididymal spermatozoa. Mol. Reprod. Dev. 2002, 61, 49-56.
21. Nakayama, A.; Sato, M.; Shinohara, M.; Matsubara, S.; Yokomine, T.; Akasaka, E.; Yoshida, M.; Takao, S. Efficient transfection of primarily cultured porcine embryonic fibroblasts using the Amaxa nucleofection system TM. Cloning Stem Cells 2007, 9, 523-534.