A rapid approach for characterization of thiol-conjugated antibody-drug conjugates and calculation of drug-antibody ratio by liquid chromatography mass spectrometry

Analytical Biochemistry

Volumn 485, Issue , 2015, Pages 34-42

  Firth, David ^a   Bell, Leonard ^a   Squires, Martin ^a   Estdale, Sian ^a   McKee, Colin ^b  

^a UK   (United Kingdom)

^b ADC Biotechnology   (United Kingdom)

Author keywords

ADC; Antibody; Characterization; DAR; LC MS; Mass spectrometry

Indexed keywords

AMINO ACIDS; ANTIBODIES; DECISION MAKING; MASS SPECTROMETRY;

ANTIBODY-DRUG CONJUGATES; CONJUGATION CHEMISTRY; LC-MS; LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIES; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; ORTHOGONAL METHODOLOGY; STREPTOCOCCUS PYOGENES; VISUAL REPRESENTATIONS;

LIQUID CHROMATOGRAPHY;

ANTIBODY CONJUGATE; DRUG ANTIBODY; MALEIMIDOCAPROYL MONOMETHYL AURISTATIN F ANTIBODY; THIOL DERIVATIVE; UNCLASSIFIED DRUG; VALINE CITRULLINE MONOMETHYL AURISTATIN E ANTIBODY; AMINOBENZOIC ACID DERIVATIVE; ANTIBODY; AURISTATIN; BACTERIAL PROTEIN; MAC-1-LIKE PROTEIN, STREPTOCOCCUS; OLIGOPEPTIDE;

ANTIBODY STRUCTURE; ARTICLE; CONTROLLED STUDY; DEGLYCOSYLATION; DRUG CONJUGATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PRIORITY JOURNAL; PROCESS DEVELOPMENT; STREPTOCOCCUS PYOGENES; CHEMISTRY; ENZYMOLOGY;

STREPTOCOCCUS PYOGENES;

AMINOBENZOATES; ANTIBODIES; BACTERIAL PROTEINS; CHROMATOGRAPHY, LIQUID; MASS SPECTROMETRY; OLIGOPEPTIDES; STREPTOCOCCUS PYOGENES; SULFHYDRYL COMPOUNDS;

EID: 84936869308     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2015.06.001     Document Type: Article

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