Detection and quantitation of afucosylated n-linked oligosaccharides in recombinant monoclonal antibodies using enzymatic digestion and LC-MS

Journal of the American Society for Mass Spectrometry

Volumn 23, Issue 7, 2012, Pages 1241-1249

  Du, Yi ^a   May, Kimberly ^a   Xu, Wei ^a   Liu, Hongcheng ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Afucosylation; Glycosylation; LC MS; Recombinant monoclonal antibody

Indexed keywords

AFUCOSYLATION; ANTIBODY-DEPENDENT CELLULAR CYTOTOXICITIES; BINDING AFFINITIES; BIOLOGICAL FUNCTIONS; ENZYMATIC DIGESTIONS; GLYCOPEPTIDES; GLYCOSIDIC BOND; LC-MS; LC-MS ANALYSIS; N-LINKED; OLIGOSACCHARIDE STRUCTURE; RECOMBINANT MONOCLONAL ANTIBODY; SIGNIFICANT IMPACTS; TRYPSIN DIGESTION; TRYPTIC PEPTIDES;

BINDING ENERGY; GLYCOSYLATION; MONOCLONAL ANTIBODIES; PEPTIDES; SURFACE PLASMON RESONANCE;

OLIGOSACCHARIDES;

FC RECEPTOR; FUCOSE; GLYCOPEPTIDE; MANNOSE; MONOCLONAL ANTIBODY; N ACETYL BETA GLUCOSAMINIDASE; N ACETYLGLUCOSAMINE; OLIGOSACCHARIDE; TRYPSIN;

ACCURACY; AFUCOSYLATION; ANTIBODY DEPENDENT CELLULAR CYTOTOXICITY; ARTICLE; BINDING AFFINITY; CARBOHYDRATE ANALYSIS; CHEMICAL BOND; CHEMICAL MODIFICATION; DRUG STABILITY; DRUG STRUCTURE; ENZYME DEGRADATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PROTEIN DEGRADATION;

ANIMALS; ANTIBODIES, MONOCLONAL; CARBOHYDRATE SEQUENCE; CHO CELLS; CHROMATOGRAPHY, LIQUID; CRICETINAE; CRICETULUS; FUCOSE; GLYCOPEPTIDES; GLYCOSYLATION; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; PEPTIDE FRAGMENTS; RECOMBINANT PROTEINS; TRYPSIN;

EID: 84865847491     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-012-0397-8     Document Type: Article

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