Detection and quantitation of succinimide in intact protein via hydrazine trapping and chemical derivatization

Journal of Pharmaceutical Sciences

Volumn 103, Issue 10, 2014, Pages 3033-3042

  Klaene, Joshua J ^a   Ni, Wenqin ^a   Alfaro, Joshua F ^b   Zhou, Zhaohui Sunny ^a  

^a NORTHEASTERN UNIVERSITY   (United States)

^b WASHINGTON STATE UNIVERSITY   (United States)

Author keywords

Chemical stability; Deamidation; Formulation; Isoaspartic acid; Mass spectrometry; Posttranslational modification; Protein; Succinimide

Indexed keywords

HYDRAZINE DERIVATIVE; PROTEIN; SUCCINIMIDE; SUCCINIMIDE DERIVATIVE;

CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; ULTRAVIOLET SPECTROPHOTOMETRY;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDRAZINES; MASS SPECTROMETRY; PROTEINS; SPECTROPHOTOMETRY, ULTRAVIOLET; SUCCINIMIDES;

EID: 84930173832     PISSN: 00223549     EISSN: 15206017     Source Type: Journal    
DOI: 10.1002/jps.24074     Document Type: Article

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