Direct identification and quantification of aspartyl succinimide in an IgG2 mAb by RapiGest assisted digestion

Analytical Chemistry

Volumn 81, Issue 4, 2009, Pages 1686-1692

  Huang, Holly Z ^a   Nichols, Andrew ^a   Liu, Dingjiang ^a  

^a AMGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACID-LABILE; ACIDIC CONDITIONS; CATION-EXCHANGE CHROMATOGRAPHIES; CONVENTIONAL METHODS; DEAMIDATION; DIGESTION METHODS; DIRECT IDENTIFICATIONS; ENZYMATIC DIGESTIONS; GUANIDINE HYDROCHLORIDES; MONOCLONAL ANTIBODIES (MABS); PROTEIN MOLECULES; RAPID IDENTIFICATIONS; SAMPLE PREPARATIONS; SITE SPECIFICS; SUCCINIMIDE; TRYPTIC DIGESTIONS; TRYPTIC PEPTIDES;

AMINES; CHEMICAL MODIFICATION; CHROMATOGRAPHIC ANALYSIS; DESALINATION; HYDROCARBONS; MONOCLONAL ANTIBODIES; SURFACE ACTIVE AGENTS; UREA;

PROTEINS;

ASPARTIC ACID; GUANIDINE; IMMUNOGLOBULIN G2; SUCCINIMIDE; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; PEPTIDE; SUCCINIMIDE DERIVATIVE; SURFACTANT; TRYPSIN;

ARTICLE; CHEMICAL MODIFICATION; CHEMICAL STRUCTURE; DIGESTION; ION EXCHANGE CHROMATOGRAPHY; PH MEASUREMENT; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; CHO CELL; CRICETULUS; DRUG EFFECT; HAMSTER; HUMAN; METABOLISM; MOLECULAR GENETICS; PH; PROTEIN DENATURATION; SENSITIVITY AND SPECIFICITY; TIME;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; ASPARTIC ACID; CHO CELLS; CHROMATOGRAPHY, ION EXCHANGE; CRICETINAE; CRICETULUS; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN DENATURATION; SENSITIVITY AND SPECIFICITY; SUCCINIMIDES; SURFACE-ACTIVE AGENTS; TIME FACTORS; TRYPSIN;

EID: 63649161438     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac802708s     Document Type: Article

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