Precise and efficient antibody epitope determination through library design, yeast display and next-generation sequencing

Journal of Molecular Biology

Volumn 427, Issue 6, 2015, Pages 1513-1534

  Van Blarcom, Thomas ^a   Rossi, Andrea ^a   Foletti, Davide ^a   Sundar, Purnima ^a   Pitts, Steven ^a   Bee, Christine ^a   Melton Witt, Jody ^a   Melton, Zea ^a   Hasa Moreno, Adela ^a   Shaughnessy, Lee ^a,b   Telman, Dilduz ^a   Zhao, Lora ^a   Cheung, Wai Ling ^a   Berka, Jan ^a,c   Zhai, Wenwu ^a,d   Strop, Pavel ^a   Chaparro Riggers, Javier ^a   Shelton, David L ^a   Pons, Jaume ^a   Rajpal, Arvind ^a  

^a PFIZER INC   (United States)

^b Stratatech Corporation   (United States)

^c GENENTECH INC   (United States)

^d ROCHE MOLECULAR SYSTEMS   (United States)

Author keywords

alpha toxin; epitope mapping; FACS; Roche 454; Staphylococcus aureus

Indexed keywords

ALPHA TOXIN; ANTIBODY; NEUTRALIZING ANTIBODY; BACTERIAL TOXIN; EPITOPE; HEMOLYSIN; MONOCLONAL ANTIBODY; PEPTIDE LIBRARY; STAPHYLOCOCCAL ALPHA-TOXIN;

ANTIGEN BINDING; ARTICLE; DNA SEQUENCE; EPITOPE MAPPING; FLUORESCENCE ACTIVATED CELL SORTING; NEXT GENERATION SEQUENCING; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; STAPHYLOCOCCUS AUREUS; YEAST; AMINO ACID SEQUENCE; CHEMISTRY; FLOW CYTOMETRY; GENETICS; HIGH THROUGHPUT SEQUENCING; HUMAN; IMMUNOLOGY; METABOLISM; MOLECULAR GENETICS; PEPTIDE LIBRARY; PROCEDURES; PROTEIN CONFORMATION; SACCHAROMYCES CEREVISIAE; STAPHYLOCOCCAL INFECTIONS;

STAPHYLOCOCCUS AUREUS;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; BACTERIAL TOXINS; EPITOPE MAPPING; EPITOPES; FLOW CYTOMETRY; HEMOLYSIN PROTEINS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; PROTEIN CONFORMATION; SACCHAROMYCES CEREVISIAE; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS;

EID: 84924117303     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2014.09.020     Document Type: Article

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