Energetic analysis of an antigen/antibody interface: Alanine scanning mutagenesis and double mutant cycles on the HyHEL-10/lysozyme interaction

Protein Science

Volumn 8, Issue 5, 1999, Pages 958-968

  Pons, Jaume ^a   Rajpal, Arvind ^a,b   Kirsch, Jack F ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b PFIZER INC   (United States)

Author keywords

Alanine scan mutagenesis; Antibody; Antigen; Double mutant cycles; Epitope mapping; Hen egg white lysozyme; Monoclonal antibody HyHEL 10; Protein protein interaction

Indexed keywords

ALANINE; AMINO ACID; LYSOZYME; MONOCLONAL ANTIBODY; MUTANT PROTEIN;

ANTIGEN ANTIBODY COMPLEX; ARTICLE; COMPLEX FORMATION; CRYSTALLOGRAPHY; ENERGY; ENZYME ANALYSIS; EPITOPE MAPPING; HYDROGEN BOND; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS;

PICHIA PASTORIS;

EID: 0032958585     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.5.958     Document Type: Article

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