Regioselectivity-driven evolution of CYP102D1 for improved synthesis of 3'-ortho-dihydroxyisoflavone

Enzyme and Microbial Technology

Volumn 71, Issue , 2015, Pages 20-27

  Choi, Kwon Young ^a   Yang, Yung Hun ^b   Kim, Byung gee ^c  

^a Ajou University   (South Korea)

^b Konkuk University   (South Korea)

^c Seoul National University   (South Korea)

Author keywords

Cytochrome P450 monooxygenases; Daidzein; O dealkylation HTS assay; Regioselectivity; Self sufficient CYP102D1

Indexed keywords

FLAVONOIDS; HYDROXYLATION; MUTAGENESIS;

ANTI-OXIDANT ACTIVITIES; CYTOCHROME P450 MONOOXYGENASES; DAIDZEIN; HIGH THROUGHPUT SCREENING; O-DEALKYLATION; PROTEIN ENGINEERING; SATURATION MUTAGENESIS; SELF-SUFFICIENT CYP102D1;

REGIOSELECTIVITY;

3' ORTHO DIHYDROXYISOFLAVONE; CYTOCHROME P450; CYTOCHROME P450 102D1; DAIDZEIN; ISOFLAVONE DERIVATIVE; OXYGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE DEHYDROGENASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; FLAVOCYTOCHROME P450 BM3 MONOXYGENASES; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE;

ARTICLE; BACTERIAL CELL; BINDING AFFINITY; CATALYSIS; CHEMICAL REACTION KINETICS; DEALKYLATION; DRUG SCREENING; DRUG SYNTHESIS; ELECTRON TRANSPORT; ENZYME ACTIVITY; ESCHERICHIA COLI; HIGH THROUGHPUT SCREENING; HYDROXYLATION; MASS FRAGMENTOGRAPHY; MOLECULAR DOCKING; NONHUMAN; O DEALKYLATION; REGIOSELECTIVITY; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; SITE SATURATION MUTAGENESIS; AMINO ACID SUBSTITUTION; BIOSYNTHESIS; BIOTECHNOLOGY; CHEMICAL STRUCTURE; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; GENETICS; KINETICS; METABOLISM;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BIOTECHNOLOGY; CATALYTIC DOMAIN; CYTOCHROME P-450 ENZYME SYSTEM; DIRECTED MOLECULAR EVOLUTION; HIGH-THROUGHPUT SCREENING ASSAYS; HYDROXYLATION; ISOFLAVONES; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; NADPH-FERRIHEMOPROTEIN REDUCTASE; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 84922477207     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2015.01.004     Document Type: Article

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