Flavocytochrome P450 BM3 mutant W1046A is a NADH-dependent fatty acid hydroxylase: Implications for the mechanism of electron transfer in the P450 BM3 dimer

Archives of Biochemistry and Biophysics

Volumn 507, Issue 1, 2011, Pages 75-85

  Girvan, Hazel M ^a   Dunford, Adrian J ^a   Neeli, Rajasekhar ^a   Ekanem, Idorenyin S ^b   Waltham, Timothy N ^a   Joyce, M Gordon ^c   Leys, David ^a   Curtis, Robin A ^b   Williams, Paul ^a   Fisher, Karl ^a   Voice, Michael W ^d   Munro, Andrew W ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

^c UNIVERSITY OF LEICESTER   (United Kingdom)

^d Dundee DD2 1NH   (United Kingdom)

Author keywords

Coenzyme selectivity; Cytochrome P450; Electron transfer; Fatty acid hydroxylation; Flavocytochrome P450 BM3; FMN dissociation

Indexed keywords

CYTOCHROME P450 BM3; DIMER; FATTY ACID; FLAVINE MONONUCLEOTIDE; HEME; LACTATE DEHYDROGENASE (CYTOCHROME); LAURIC ACID; MONOMER; OXYGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; FLAVOCYTOCHROME P450 BM3; MUTANT PROTEIN; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG; W1046A BM3 PROTEIN;

ARTICLE; BACILLUS MEGATERIUM; BIOTECHNOLOGY; CATALYSIS; CONTROLLED STUDY; DATA ANALYSIS; DRUG HYDROXYLATION; ELECTRON TRANSPORT; HUMAN; HYPOTHESIS; MEDICAL RECORD; MUTANT; NONHUMAN; PRIORITY JOURNAL; ENZYME ACTIVITY; HYDROXYLATION; OXIDATION; PROTEIN FUNCTION;

BACILLUS MEGATERIUM; BACTERIAL PROTEINS; CYTOCHROME P-450 ENZYME SYSTEM; ELECTRON TRANSPORT; FATTY ACIDS; FLAVIN MONONUCLEOTIDE; HEME; HYDROXYLATION; MUTATION; NAD; NADPH-FERRIHEMOPROTEIN REDUCTASE; OXIDATION-REDUCTION; PROTEIN MULTIMERIZATION;

BACILLUS MEGATERIUM;

EID: 79951571691     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2010.09.014     Document Type: Article

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