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Volumn 14, Issue 10, 2013, Pages 1231-1238

Development of Colorimetric HTS Assay of Cytochrome P450 for ortho-Specific Hydroxylation, and Engineering of CYP102D1 with Enhanced Catalytic Activity and Regioselectivity

(6) Choi, Kwon Young a Jung, Eun Ok a Yun, Hyungdon b Yang, Yung Hun c Kazlauskas, Romas J d Kim, Byung Gee a

a Seoul National University (South Korea)

b Yeungnam University (South Korea)

c Konkuk University (South Korea)

d UNIVERSITY OF MINNESOTA (United States)

Author keywords

CYP102D1; cytochromes; diol sensing; HTS assay; hydroxylation; O dealkylation

Indexed keywords

ALDEHYDE; AROMATIC COMPOUND; CARBON; CYP102D1; CYTOCHROME P450; DAIDZEIN; FORMALDEHYDE; UNCLASSIFIED DRUG;

ARTICLE; BIOTRANSFORMATION; CATALYSIS; COLORIMETRY; DEALKYLATION; ENZYME ACTIVITY; ENZYME ENGINEERING; HIGH THROUGHPUT SCREENING; HYDROXYLATION; MOLECULAR LIBRARY; PRIORITY JOURNAL; WHOLE CELL;

CYP102D1; CYTOCHROMES; DIOL-SENSING; HTS ASSAY; HYDROXYLATION; O-DEALKYLATION;

CATALYSIS; COLORIMETRY; CYTOCHROME P-450 ENZYME SYSTEM; DEALKYLATION; HIGH-THROUGHPUT SCREENING ASSAYS; HYDROXYLATION; OXIDATION-REDUCTION; SUBSTRATE SPECIFICITY;

EID: 84879843682 PISSN: 14394227 EISSN: 14397633 Source Type: Journal
DOI: 10.1002/cbic.201300212 Document Type: Article

Times cited : (10)

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