Multiplex detection of functional G protein-coupled receptors harboring site-specifically modified unnatural amino acids

Biochemistry

Volumn 54, Issue 3, 2015, Pages 776-786

  Naganathan, Saranga ^a   Ray Saha, Sarmistha ^a,c   Park, Minyoung ^a   Tian, He ^a   Sakmar, Thomas P ^a,b   Huber, Thomas ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b KAROLINSKA INSTITUTE   (Sweden)

^c Siemens Healthcare Diagnostics Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CRYSTAL STRUCTURE; FLUOROPHORES; HYDROPHOBICITY; PEPTIDES; PROTEINS;

G PROTEIN COUPLED RECEPTORS; HYDROPHOBIC CAVITIES; LABELING EFFICIENCIES; RECEPTOR TRAFFICKING; SIGNALING MECHANISMS; SINGLE-MOLECULE DETECTION; TRANSMEMBRANE HELICES; UNNATURAL AMINO ACIDS;

EPITOPES;

ALKYNE; AMINO ACID; AZIDE; CELL SURFACE RECEPTOR; CHEMOKINE RECEPTOR CCR5; G PROTEIN COUPLED RECEPTOR; MARAVIROC; PHENYLALANINE; POLYCLONAL ANTIBODY; UNCLASSIFIED DRUG; UNNATURAL AMINO ACID; 4-AZIDOPHENYLALANINE; CYCLOHEXANE DERIVATIVE; EPITOPE; FLUORESCENT DYE; TRIAZOLE DERIVATIVE;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOCOMPATIBILITY; CARBOXY TERMINAL SEQUENCE; CELL LYSATE; CELL SURFACE; CODON; CRYSTAL STRUCTURE; CYCLOADDITION; ENZYME LINKED IMMUNOSORBENT ASSAY; HEK293 CELL LINE; HUMAN; HUMAN CELL; MULTIPLEX DETECTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; SITE DIRECTED MUTAGENESIS; SITE SPECIFIC AMBER CODON MUTATION; STOICHIOMETRY; WESTERN BLOTTING; WILD TYPE; ANALOGS AND DERIVATIVES; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; IMMUNOASSAY; METABOLISM; MUTATION; PROCEDURES;

AMINO ACIDS; AZIDES; CYCLOHEXANES; EPITOPES; FLUORESCENT DYES; HEK293 CELLS; HUMANS; IMMUNOASSAY; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHENYLALANINE; RECEPTORS, CCR5; TRIAZOLES;

EID: 84922021482     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi501267x     Document Type: Article

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