Mapping Substance P binding sites on the neurokinin-1 receptor using genetic incorporation of a photoreactive amino acid

Journal of Biological Chemistry

Volumn 289, Issue 26, 2014, Pages 18045-18054

  Valentin Hansen, Louise ^a,b   Park, Minyoung ^b,c   Huber, Thomas ^c   Grunbeck, Amy ^c   Naganathan, Saranga ^c   Schwartz, Thue W ^a,b   Sakmar, Thomas P ^b,c  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b Capital Region   (Denmark)

^c ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; LIGANDS; MAMMALS; PHOTOREACTIVITY;

CELL-BASED ASSAYS; EXTRACELLULAR LOOPS; G PROTEIN COUPLED RECEPTORS; MULTIPLE DOMAINS; PHOTOAFFINITY LABELING; PHOTOCROSS-LINKING; PHOTOCROSS-LINKING APPROACH; PHYSIOLOGICAL RESPONSE;

BINDING SITES;

4 BENZOYL PHENYLALANINE; AMINO ACID; G PROTEIN COUPLED RECEPTOR; NEUROKININ 1 RECEPTOR; SUBSTANCE P; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CODON; CONTROLLED STUDY; GENETIC TRANSFECTION; HEK293 CELL LINE; IMMUNOBLOTTING; MOLECULAR BIOLOGY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CROSS LINKING; PROTEIN INTERACTION;

AMBER CODON SUPPRESSION; G PROTEIN-COUPLED RECEPTOR (GPCR); HOMOLOGY MODELING; MEMBRANE PROTEIN; PHOTOACTIVE UNNATURAL AMINO ACID; PROTEIN CROSS-LINKING; STRUCTURAL BIOLOGY;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; HUMANS; MODELS, MOLECULAR; PHOTOAFFINITY LABELS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, NEUROKININ-1; SUBSTANCE P; ULTRAVIOLET RAYS;

EID: 84903542429     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M113.527085     Document Type: Article

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