Direct measurement of thermal stability of expressed CCR5 and stabilization by small molecule ligands

Biochemistry

Volumn 50, Issue 4, 2011, Pages 502-511

  Knepp, Adam M ^a   Grunbeck, Amy ^a   Banerjee, Sourabh ^a   Sakmar, Thomas P ^a   Huber, Thomas ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

APOLIPOPROTEINS; ASSAY TECHNOLOGIES; BI-LAYER; BIOPHYSICAL STUDIES; CHEMOKINE RECEPTORS; DETERGENT SOLUTION; DIRECT MEASUREMENT; FEMTOMOLE QUANTITIES; G PROTEIN COUPLED RECEPTORS; HIGH RESOLUTION CRYSTAL STRUCTURE; HIGH YIELD; HIGH-THROUGHPUT; HOMOGENEOUS TIME-RESOLVED FLUORESCENCE ASSAYS; INHERENT INSTABILITY; MEMBRANE PROTEINS; NANO SCALE; NEW APPROACHES; SMALL MOLECULES; STRUCTURAL STUDIES; THERMAL STABILITY;

BIOLOGICAL MEMBRANES; CRYSTAL STRUCTURE; DETERGENTS; PROTEINS; PURIFICATION; THERMODYNAMIC STABILITY;

STABILIZATION;

4 [1 [(2,4 DIMETHYL 3 PYRIDINYL)CARBONYL] 4 METHYL 4 PIPERIDINYL] 2 METHYL 1 [1 [4 (TRIFLUOROMETHYL)PHENYL]ETHYL]PIPERAZINE; CHEMOKINE RECEPTOR CCR5; DETERGENT; MARAVIROC; N [3 [4 (3 BENZYL 1 ETHYL 5 PYRAZOLYL) 1 PIPERIDINYLMETHYL] 4 (3 FLUOROPHENYL)CYCLOPENTYL] N METHYLVALINE; NANOPARTICLE; NANOSCALE APOLIPOPROTEIN BOUND BILAYER PARTICLE; UNCLASSIFIED DRUG; VICRIVIROC;

ARTICLE; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; HIGH THROUGHPUT SCREENING; HOMOGENEOUS TIME RESOLVED FLUORESCENCE ASSAY; HUMAN; HUMAN CELL; MEASUREMENT; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN STABILITY; THERMOSTABILITY;

ANTIBODIES, MONOCLONAL; CRYSTALLOGRAPHY, X-RAY; CYCLOHEXANES; FLUORESCEIN; HEK293 CELLS; HUMANS; IMMUNOBLOTTING; LIGANDS; LIPID BILAYERS; NIACINAMIDE; PIPERAZINES; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN STABILITY; PYRIMIDINES; RECEPTORS, CCR5; SOLUBILITY; THERMODYNAMICS; TRIAZOLES;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 79952081606     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi101059w     Document Type: Article

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