High-throughput nucleoside phosphate monitoring in mammalian cell fed-batch cultivation using quantitative matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Biotechnology Journal

Volumn 10, Issue 1, 2015, Pages 190-198

  Steinhoff, Robert F ^a   Ivarsson, Marija ^a   Habicher, Tobias ^a,b   Villiger, Thomas K ^a   Boertz, Jens ^c   Krismer, Jasmin ^a   Fagerer, Stephan R ^a   Soos, Miroslav ^a   Morbidelli, Massimo ^a   Pabst, Martin ^a   Zenobi, Renato ^a  

^a ETH ZURICH   (Switzerland)

^b GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^c Sigma Aldrich Chemie GmbH   (Switzerland)

Author keywords

ATP; Automated sample aliquoting; MALDI MS; Microarrays for mass spectrometry (MAMS); Monoclonal antibody production

Indexed keywords

ADENOSINETRIPHOSPHATE; ANTIBODIES; BATCH DATA PROCESSING; BIOASSAY; BIOCONVERSION; BIOMOLECULES; BIOREACTORS; CELL CULTURE; CELL PROLIFERATION; CELLS; CULTIVATION; CYTOLOGY; DESORPTION; HYPOTHERMIA; INDUCTIVELY COUPLED PLASMA; ISOTOPES; MAMMALS; METABOLITES; MICROARRAYS; MONITORING; MONOCLONAL ANTIBODIES; PRINCIPAL COMPONENT ANALYSIS; SPECTROMETRY; THROUGHPUT;

ALIQUOTING; BIOTECHNOLOGICAL PROCESS; INTRACELLULAR METABOLITES; MALDI-MS; MATRIX ASSISTED LASER DESORPTION/IONIZATION; MONOCLONAL ANTIBODY PRODUCTION; STABLE ISOTOPE LABELED INTERNAL STANDARDS; TIME OF FLIGHT MASS SPECTROMETRY;

MASS SPECTROMETRY;

GLUTATHIONE; INDIUM TIN OXIDE; NUCLEOSIDE; NUCLEOTIDE; PHOSPHATE; STABLE ISOTOPE; ADENOSINE TRIPHOSPHATE;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL PROLIFERATION; FED BATCH CULTURE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYBRIDOMA CELL CULTURE; HYBRIDOMA CELL LINE; HYPEROSMOLARITY; HYPOTHERMIA; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; MAMMAL CELL; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOUSE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE STUDY; ULTRAVIOLET RADIATION; VALIDATION STUDY; ANIMAL; CELL LINE; CELL SURVIVAL; CULTURE TECHNIQUE; INTRACELLULAR SPACE; MASS SPECTROMETRY; METABOLISM; METABOLOMICS; PRINCIPAL COMPONENT ANALYSIS; PROCEDURES;

MAMMALIA;

ADENOSINE TRIPHOSPHATE; ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; CELL SURVIVAL; INTRACELLULAR SPACE; METABOLOMICS; MICE; PRINCIPAL COMPONENT ANALYSIS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84921496117     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201400292     Document Type: Article

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