Process parameter shifting: Part I. Effect of DOT, pH, and temperature on the performance of Epo-Fc expressing CHO cells cultivated in controlled batch bioreactors

Biotechnology and Bioengineering

Volumn 94, Issue 6, 2006, Pages 1033-1044

  Trummer, Evelyn ^a,c   Fauland, Katharina ^a,c   Seidinger, Silke ^a,c   Schriebl, Kornelia ^a,c   Lattenmayer, Christine ^a,c   Kunert, Renate ^a,c   Vorauer Uhl, Karola ^a,c   Weik, Robert ^b   Borth, Nicole ^a   Katinger, Hermann ^a,b   Müller, Dethardt ^a,b,c  

^a UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

^b POLYMUN SCIENTIFIC IMMUNBIOLOGISCHE FORSCHUNG GMBH   (Austria)

^c Austrian Center of Biopharmaceutical Technology   (Austria)

Author keywords

Controlled bioreactor; Integral of viable cells; Process parameter shift; Product quality; Recombinant CHO cells; Specific productivity

Indexed keywords

BIOREACTORS; CELLS; METABOLISM; OPTIMIZATION; PH EFFECTS; PHYSIOLOGY;

CONTROLLED BIOREACTOR; INTEGRAL OF VIABLE CELLS; PROCESS PARAMETER SHIFT; PRODUCT QUALITY; RECOMBINANT CHO CELLS; SPECIFIC PRODUCTIVITY;

PROCESS ENGINEERING;

DISSOLVED OXYGEN; HYBRID PROTEIN; PROTEIN EPO FC; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL CULTURE; CELL CYCLE; CELL GROWTH; CELL METABOLISM; CELL PROLIFERATION; CELL VIABILITY; CHO CELL; CONTROLLED STUDY; DNA MODIFICATION; GLYCOSYLATION; HOST CELL; MAMMAL CELL; METABOLIC ACTIVATION; NONHUMAN; PH MEASUREMENT; PROTEIN EXPRESSION; SIALYLATION; TEMPERATURE SENSITIVITY;

ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; CHO CELLS; COMPUTER SIMULATION; CRICETINAE; CRICETULUS; ERYTHROPOIETIN; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; MODELS, BIOLOGICAL; OXYGEN; OXYGEN CONSUMPTION; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; TEMPERATURE;

MAMMALIA;

EID: 33747126481     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21013     Document Type: Article

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