Estimating cell specific oxygen uptake and carbon dioxide production rates for mammalian cells in perfusion culture

Biotechnology Progress

Volumn 27, Issue 5, 2011, Pages 1347-1357

  Goudar, Chetan T ^a   Piret, James M ^b   Konstantinov, Konstantinov B ^a,c  

^a Global Biological Development   (United States)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c GENZYME CORPORATION   (United States)

Author keywords

Cell culture; CHO cells; Metabolic flux analysis; Q CO2; Q O2; RQ

Indexed keywords

CHO CELL; METABOLIC FLUX ANALYSIS; Q CO2; Q O2; RQ;

ANIMAL CELL CULTURE; CARBON; CELL CULTURE; CELLS; CYTOLOGY; DISSOLVED OXYGEN; ESTIMATION; MAMMALS; PHYSIOLOGY;

CARBON DIOXIDE;

CARBON DIOXIDE; GLUCOSE; LACTIC ACID DERIVATIVE; OXYGEN; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CELL DIVISION; CHO CELL; CRICETULUS; HAMSTER; METABOLISM; PERFUSION; PH;

ANIMALS; CARBON DIOXIDE; CELL DIVISION; CELLS, CULTURED; CHO CELLS; CRICETINAE; CRICETULUS; GLUCOSE; HYDROGEN-ION CONCENTRATION; LACTATES; OXYGEN; PERFUSION; RECOMBINANT PROTEINS;

CRICETULUS GRISEUS; MAMMALIA;

EID: 80053897817     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.646     Document Type: Article

References (58)

1. Bonarius HP, de Gooijer CD, Tramper J, Schmid G. Determination of the respiration quotient in mammalian cell culture in bicarbonate buffered media. Biotechnol Bioeng. 1995; 45: 524-535.
2. Miller WM, Wilke CR, Blanch HW. Effects of dissolved oxygen concentration on hybridoma growth and metabolism in continuous cultures. J Cell Physiol. 1987; 132: 524-530.
3. Ogawa T, Kamihira M, Yoshida H, Iijima H, Kobayashi T. Effect of dissolved oxygen concentration on monoclonal antibody production in hybridoma cell cultures. J Ferm Bioeng. 1992; 74: 372-378.
4. Ozturk S, Palsson BO. Effects of dissolved oxygen on hybridoma cell growth, metabolism, and antibody produciton kinetics in continuous culture. Biotechnol Prog. 1990; 6: 437-446.
5. Philips HA, Scharer JM, Bols NC, Moo-Young M. Effect of oxygen on antibody production in hybridoma culture. Biotechnol Lett. 1987; 9: 745-750.
6. 2) oxygen uptake rates in animal cell cultures. J Biotechnol. 1998; 63: 85-95.
7. Shi Y, Ryu DDY, Park SH. Monoclonal-antibody productivity and the metabolic pattern of perfusion cultures under varying oxygen-tensions. Biotechnol Bioeng. 1993; 42: 430-439.
8. Vallejos JR, Brorson KA, Moreira AR, Rao G. Dissolved oxygen and pH profile evolution after cryovial thaw and repeated cell passaging in a T-75 flask. Biotechnol Bioeng. 2010; 105: 1040-1047.
9. Yamada K, Furushou S, Sugahara T, Shirahata S, Murakami H. Relationship between oxygen consumption rate and cellular activity of mammalian cells cultured in serum-free media. Biotechnol Bioeng. 1990; 36: 759-762.
10. Feng Q, Mi L, Li L, Liu R, Xie L, Tang H, Chen Z. Application of "oxygen uptake rate-amino acids" associated mode in controlled-fed perfusion culture. J Biotechnol. 2006; 122: 422-430.
11. Kyung Y-S, Peshwa MV, Gryte DM, Hu WS. High density culture of mammalian cells with dynamic perfusion based on on-line oxygen uptake measurements. Cytotechnology. 1994; 14: 183-190.
12. Zhou WC, Hu WS. On-line characterization of a hybridoma cell culture process. Biotechnol Bioeng. 1994; 44: 170-177.
13. Balcarcel RR, Clark LM. Metabolic screening of mammalian cell cultures using well-plates. Biotechnol Prog. 2003; 19: 98-108.
14. Deshpande R, Heinzle E. Online monitoring of oxygen in spinner flasks. Biotechnol Lett. 2009; 31: 665-669.
15. Deshpande RR, Heinzle E. On-line oxygen uptake rate and culture viability measurement of animal cell culture using microplates with integrated oxygen sensors. Biotechnol Lett. 2004; 26: 763-767.
16. Eyer K, Oeggerli A, Heinzle E. On-line gas analysis in animal cell cultivation. II. Methods for oxygen uptake rate estimation and its application to controlled feeding of glutamine. Biotechnol Bioeng. 1995; 45: 54-62.
17. Singh V. On-line measurement of oxygen uptake in cell culture using the dynamic method. Biotechnol Bioeng. 1996; 52: 443-448.
18. Yoon S, Konstantinov K. Continuous, real-time monitoring of the oxygen uptake rate (OUR) in animal cell bioreactors. Biotechnol Bioeng. 1994; 44: 983-990.
19. Aiba S, Furuse H. Some comments on respiratory quotient (RQ) determination from the analysis of exit gas from a fermentor. Biotechnol Bioeng. 1990; 36: 534-538.
20. Ho CS, Smith MD, Shanahan JF. Carbon dioxide transfer in biochemical reactors. Adv Biochem Eng. 1987; 35: 83-125.
21. Minkevich IG, Neubert M. Influence of carbon dioxide solubility on the accuracy of measurements of carbon dioxide production rate by gas balance technique. Acta Biotechnol. 1985; 5: 137-143.
22. Royce PN. Effect of changes in the pH and carbon dioxide evolution rate on the measured respiratory quotient of fermentations. Biotechnol Bioeng. 1992; 40: 1129-1138.
23. Royce PN, Thornhill NF. Estimation of dissolved carbon dioxide concentrations in aerobic fermentations. AIChE J. 1991; 37: 1680-1685.
24. 2 evolution rate in aerobic and anerobic continuous cultures. Appl Microbiol Biotechnol. 1995; 42: 688-691.
25. 2 production rate of mammalian cell suspension culture based on off-gas measurement. J Biotechnol. 2002; 99: 133-148.
26. Lovrecz G, Gray P. Use of on-line gas analysis to monitor recombinant mammalian cell cultures. Cytotechnology. 1994; 14: 167-175.
27. Stumm W, Morgan JJ. Aquatic Chemistry, 3rd ed. New York: Wiley; 1996.
28. Snoeyink VL, Jenkins D. Water Chemistry. New York, NY: Wiley; 1980.
29. Morel FMM, Hering JG. Principles and Applications of Aquatic Chemistry. New York, NY: Wiley; 1993.
30. Butler JN. Ionic Equilibrium: Solubility and pH Calculations. New York, NY: Wiley; 1998.
31. 2 accumulation by eliminating bicarbonate addition to high cell-density cultures. Biotechnol Bioeng. 2007; 96: 1107-1117.
32. Schumpe A, Quicker G, Deckwer WD. Gas solubilities in microbial culture media. Adv Biochem Eng. 1982; 24: 1-38.
33. Goudar CT, Biener R, Konstantinov K, Piret JM. Error propagation from prime variables into specific rates and metabolic fluxes for mammalian cells in perfusion culture. Biotechnol Prog. 2009; 25: 986-998.
34. Chuppa S, Tsai SP, Yoon S, Shackelford S, Rozales C, Bhat R, Tsay G, Matanguihan R, Konstantinov K, Naveh D. Fermentor temperature as a tool for control of high-density perfusion cultures of mammalian cells. Biotechnol Bioeng. 1997; 55: 328-338.
35. McQueen A, Bailey JE. Effect of ammonium ion and extracellular pH on hybridoma cell metabolism amd antibody production. Biotechnol Bioeng. 1990; 35: 1067-1077.
36. Europa AF, Gambhir A, Fu PC, Hu WS. Multiple steady states with distinct cellular metabolism in continuous culture of mammalian cells. Biotechnol Bioeng. 2000; 67: 25-34.
37. Follstad BD, Balcarcel RR, Stephanopoulos G, Wang DI. Metabolic flux analysis of hybridoma continuous culture steady state multiplicity. Biotechnol Bioeng. 1999; 63: 675-683.
38. Goudar CT, Biener R, Meneses R, Zhang C, Michaels J, Piret JM, Konstantinov K. Towards industrial application of real-time metabolic flux analysis for animal cell culture. Adv Biochem Eng. 2006; 101: 99-118.
39. Nyberg GB, Balcarcel RR, Follstad BD, Stephanopoulos G, Wang DI. Metabolism of peptide amino acids by Chinese hamster ovary cells grown in a complex medium. Biotechnol Bioeng. 1999; 62: 324-335.
40. Jan DCH, Petch DA, Huzel N, Butler M. The effect of dissolved oxygen on the metabolic profile of a murine hybridoma grown in serum-free medium in continuous culture. Biotechnol Bioeng. 1997; 54: 153-164.
41. Kilburn DG, Lilly MD, Self DA, Webb FC. The effect of dissolved oxygen partial pressure on the growth and carbohydrate metabolism of mouse LS cells. J Cell Sci. 1969; 4: 25-37.
42. Zupke C, Sinskey AJ, Stephanopoulos G. Intracellular flux analysis applied to the effect of dissolved oxygen on hybridomas. Appl Microbiol Biotechnol. 1995; 44: 27-36.
43. Kilburn DG, Webb FC. The cultivation of animal cells at controlled dissolved oxygen partial pressure. Biotechnol Bioeng. 1968; 10: 801-814.
44. Bonarius HP, Houtman JH, Schmid G, de Gooijer CD, Tramper J. Metabolic-flux analysis of hybridoma cells under oxidative and reductive stress using mass balance. Cytotechnology. 2000; 32: 97-107.
45. Pugh GG. The role of oxygen consumption rate in bioreactor process control. Bio/Technol. 1988; 6: 524-526.
46. Jorjani P, Ozturk S. Effects of cell density and temperature on oxygen consumption rate for different mammalian cell lines. Biotechnol Bioeng. 1999; 64: 349-356.
47. Moore A, Mercer J, Dutina G, Donahue CJ, Bauer KD, Mather JP, Etcheverry T, Ryll T. Effects of temperature shift on cell cycle, apoptosis and nucleotide pools in CHO cell batch cultures. Cytotechnology. 1997; 23: 47-54.
48. Ducommun P, Ruffieux P, Kadouri A, Stockar U, Marison I. Monitoring of temperature effects on animal cell metabolism in a packed bed process. Biotechnol Bioeng. 2002; 77: 838-842.
49. McQueen A, Bailey JE. Growth inhibition of hybridoma cells by ammonium ion: correlation with effect on intracellular pH. Bioprocess Eng. 1990; 6: 49-61.
50. McQueen A, Bailey JE. Mathematical modeling of the effects of ammonium ion on the intracellular pH of hybridoma cells. Biotechnol Bioeng. 1990; 35: 897-906.
51. Miller WM, Blanch HW, Wilke CR. A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: effect of nutrient concentration, dilution rate and pH. Biotechnol Bioeng. 1987; 32: 947-965.
52. Backer MP, Metzger LS, Slaber PL, Nevitt KL, Boder GB. Large-scale production of monoclonal antibodies in suspension culture. Biotechnol Bioeng. 1988; 32: 993-1000.
53. Ramirez OT, Mutharasan R. Cell cycle and growth phase dependent variations in size distribution, antibody productivity, and oxygen demand in hybridoma cultures. Biotechnol Bioeng. 1990; 36: 839-848.
54. Siano SA, Mutharasan R. NADH fluorescence and oxygen uptake responses of hybridoma cultures to substrate pulse and step changes. Biotechnol Bioeng. 1991; 37: 141-159.
55. Xie L, Wang DIC. Energy metabolism and ATP balance in animal cell cultivation using a stoichiometrically based reaction network. Biotechnol Bioeng. 1996; 52: 591-601.
56. 13C-Lactate nuclear magnetic resonance spectroscopy and metabolite balancing. Biotechnol Bioeng. 2001; 74: 528-538.
57. Ahmet O, Heinzle E. Measurement of oxygen uptake and carbon dioxide production rates of mammalian cells using membrane mass spectrometry. Cytotechnology. 2001; 37: 153-162.
58. Wang NS, Stephanopoulos G. Application of macroscopic balances to the identification of gross measurement errors. Biotechnol Bioeng. 1983; 25: 2177-2208.