Metabolomics-based identification of apoptosis-inducing metabolites in recombinant fed-batch CHO culture media

Journal of Biotechnology

Volumn 151, Issue 2, 2011, Pages 218-224

  Chong, William P K ^a,b   Yusufi, Faraaz N K ^a   Lee, Dong Yup ^a,c   Reddy, Satty G ^a   Wong, Niki S C ^a   Heng, Chew Kiat ^b   Yap, Miranda G S ^a,c   Ho, Ying Swan ^a  

^a AGENCY FOR SCIENCE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Apoptosis; Chinese hamster ovary; Liquid chromatography mass spectrometry; Metabolic engineering; Metabolomics

Indexed keywords

APOPTOSIS; CHINESE HAMSTER OVARY; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; METABOLIC ENGINEERING; METABOLOMICS;

AMINO ACIDS; ANIMAL CELL CULTURE; BIOMOLECULES; CELL CULTURE; CELL DEATH; CHROMATOGRAPHY; ENZYME ACTIVITY; HIGH PRESSURE LIQUID CHROMATOGRAPHY; LIQUIDS; MAMMALS; MASS SPECTROMETRY; METABOLITES;

METABOLISM;

AMINO ACID; CASPASE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CULTURE; CHO CELL; ENZYME ACTIVITY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLOMICS; NONHUMAN; PRIORITY JOURNAL;

ADENOSINE; ADENOSINE TRIPHOSPHATE; ANIMALS; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; BIOREACTORS; CASPASES; CELL CYCLE; CHO CELLS; CHROMATOGRAPHY, LIQUID; CRICETINAE; CRICETULUS; MASS SPECTROMETRY; METABOLOMICS; RECOMBINANT PROTEINS;

CRICETULUS GRISEUS; MAMMALIA;

EID: 78651480501     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.12.010     Document Type: Article

References (46)

1. Alnemri E.S. Mammalian cell death proteases: a family of highly conserved aspartate specific cysteine proteases. J. Cell. Biochem. 1997, 64(1):33-42.
2. Andrade R., Crisol L., Prado R., Boyano M.D., Arluzea J., Aréchaga J. Plasma membrane and nuclear envelope integrity during the blebbing stage of apoptosis: a time-lapse study. Biol. Cell. 2009, (July 27).
3. Böger R.H., Bode-Böger S.M., Tsao P.S., Lin P.S., Chan J.R., Cooke J.P. An endogenous inhibitor of nitric oxide synthase regulates endothelial adhesiveness for monocytes. J. Am. Coll. Cardiol. 2000, 36:2287-2295.
4. Bresgen N., Rolinek R., Hochleitner E., Lottspeich F., Eckl P.M. Induction of apoptosis by a hepatocyte conditioned medium. J. Cell. Physiol. 2004, 198:452-460.
5. Burg M.B. Molecular basis for osmoregulation of organic osmolytes in renal medullary cells. J. Exp. Zool. 1994, 268:171-175.
6. Carvalhal A.V., Santos S.S., Calado J., Haury M., Carrondo M.J. Cell growth arrest by nucleotides, nucleosides and bases as a tool for improved production of recombinant proteins. Biotechnol. Prog. 2003, 19:69-83.
7. Cebeci, O., Budak, H., 2009. Global expression patterns of three festuca species exposed to different doses of glyphosate using the Affymetrix GeneChip wheat genome array. Comp. Funct. Genomics 505701.
8. Chen X.G., Wang R.S., Deng M.X., Ran X.Z. Effects of exogenerous nucleotides on the apoptosis of intestinal epithelial cells IEC-6. Wei Sheng Yan Jiu 2005, 34:701-704.
9. Chong W.P., Goh L.T., Reddy S.G., Yusufi F.N., Lee D.Y., Wong N.S., Heng C.K., Yap M.G., Ho Y.S. Metabolomics profiling of extracellular metabolites in recombinant Chinese Hamster Ovary fed-batch culture. Rapid Commun. Mass Spectrom. 2009, 23:3763-3771.
10. Chrysanthopoulos P.K., Goudar C.T., Klapa M.I. Metabolomics for high-resolution monitoring of the cellular physiological state in cell culture engineering. Metab. Eng. 2010, 12:212-222.
11. Circu M.L., Aw T.Y. Glutathione and apoptosis. Free Radic. Res. 2008, 42:689-706.
12. Dayoub H., Rodionov R.N., Lynch C., Cooke J.P., Arning E., Bottiglieri T., Lentz S.R., Faraci F.M. Overexpression of dimethylarginine dimethylaminohydrolase inhibits asymmetric dimethylarginine-induced endothelial dysfunction in the cerebral circulation. Stroke 2008, 39:180-184.
13. Degterev A., Boyce M., Yuan J. A decade of caspases. Oncogene 2003, 22:8543-8567.
14. Dunwiddie T.V., Diao L., Proctor W.R. Adenine nucleotides undergo rapid, quantitative conversion to adenosine in the extracellular space in rat hippocampus. J. Neurosci. 1997, 17:7673-7682.
15. Dunwiddie T.V., Masino S.A. The role and regulation of adenosine in the central nervous system. Annu. Rev. Neurosci. 2001, 24:31-55.
16. Durocher Y., Butler M. Expression systems for therapeutic glycoprotein production. Curr. Opin. Biotechnol. 2009, 20:700-707.
17. Filomeni G., Rotilio G., Ciriolo M.R. Glutathione disulfide induces apoptosis in U937 cells by a redox-mediated p38 MAP kinase pathway. FASEB J. 2003, 17:64-66.
18. Forman H.J., Zhang H., Rinna A. Glutathione: overview of its protective roles, measurement, and biosynthesis. Mol. Aspects Med. 2008, 30:1-12.
19. Franco R., Schoneveld O.J., Pappa A., Panayiotidis M.I. The central role of glutathione in the pathophysiology of human diseases. Arch. Physiol. Biochem. 2007, 113:234-258.
20. Fujimori H., Yasuda M., Pan-Hou H. Enhancement of cellular adenosine triphosphate levels in PC12 cells by extracellular adenosine. Biol. Pharm. Bull. 2002, 25:307-311.
21. Glacken M.W., Fleischaker R.J., Sinskey A.J. Reduction of waste secretion via nutrient control: possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells. Biotech. Bioeng. 1986, 28:1376-1389.
22. Hirrlinger J., Dringen R. Multidrug resistance protein 1-mediated export of glutathione and glutathione disulfide from brain astrocytes. Methods Enzymol. 2005, 400:395-409.
23. Holland M.J., Murphy E., Kelleher J.K. Adenosine metabolism in human skin fibroblasts. Am. J. Physiol. 1985, 248:C21-C26.
24. Jiang D.J., Jia S.J., Dai Z., Li Y.J. Asymmetric dimethylarginine induces apoptosis via p38 MAPK/caspase-3-dependent signaling pathway in endothelial cells. J. Mol. Cell. Cardiol. 2006, 40:529-539.
25. Kawasaki Y., Ogawa T., Sasaoka K. Occurrence and some properties of a novel gamma-glutamyltransferase responsible for the synthesis of gamma-l-glutamyl-d-alanine in pea-seedlings. Biochim. Biophys. Acta 1982, 716:194-200.
26. Kelley B. Industrialization of mAb production technology: the bioprocessing industry at a crossroads. mAbs 2009, 1:443-452.
27. Laken H.A., Leonard M.W. Understanding and modulating apoptosis in industrial cell culture. Curr. Opin. Biotechnol. 2001, 12:175-179.
28. Lee Y.Y., Yap M.G., Hu W.S., Wong K.T. Low-glutamine fed-batch cultures of 293-HEK serum-free suspension cells for adenovirus production. Biotech. Prog. 2003, 19:501-509.
29. Li Q., Harvey L.M., McNeil B. Oxidative stress in industrial fungi. Crit. Rev. Biotechnol. 2009, 29:199-213.
30. Maggon K. Monoclonal antibody gold rush. Curr. Med. Chem. 2007, 14:1978-1987.
31. Meisse D., Van de Casteele M., Beauloye C., Hainault I., Kefas B.A., Rider M.H., Foufelle F., Hue L. Sustained activation of AMP-activated protein kinase induces c-Jun N-terminal kinase activation and apoptosis in liver cells. FEBS Lett. 2002, 526:38-42.
32. Mieczkowski J., Tyburczy M.E., Dabrowski M., Pokarowski P. Probe set filtering increases correlation between Affymetrix GeneChip and qRT-PCR expression measurements. BMC Bioinform. 2010, 11:104.
33. Okajima K., Inoue M., Morino Y. Studies on the mechanism for renal elimination of N-acetylphenylalanine: its pathophysiologic significance in phenylketonuria. J. Lab. Clin. Med. 1985, 105:132-138.
34. Pandher R., Ducruix C., Eccles S.A., Raynaud F.I. Cross-platform Q-TOF validation of global exo-metabolomic analysis: application to human glioblastoma cells treated with the standard PI 3-Kinase inhibitor LY294002. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 2009, 877:1352-1358.
35. Rudolphi K.A., Schubert P., Parkinson F.E., Fredholm B.B. Neuroprotective role of adenosine in cerebral ischaemia. Trends Pharmacol. Sci. 1992, 13:439-445.
36. Schulze-Lohoff E., Hugo C., Rost S., Arnold S., Gruber A., Brüne B., Sterzel R.B. Extracellular ATP causes apoptosis and necrosis of cultured mesangial cells via P2Z/P2X7 receptors. Am. J. Physiol. 1998, 275:F962-F971.
37. Singh R.P., Al-Rubeai M., Gregory C.D., Emery A.N. Cell death in bioreactors: a role for apoptosis. Biotechnol. Bioeng. 1994, 44:720-726.
38. Smith C.A., Want E.J., O'Maille G., Abagyan R., Siuzdak G. XCMS: processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification. Anal. Chem. 2006, 78:779-787.
39. Teerlink T. ADMA metabolism and clearance. Vasc. Med. 2005, 10:S73-81.
40. Testerink N., van der Sanden M.H., Houweling M., Helms J.B., Vaandrager A.B. Depletion of phosphatidylcholine affects endoplasmic reticulum morphology and protein traffic at the Golgi complex. J. Lipid Res. 2009, 50:2182-2192.
41. van der Sanden M.H., Houweling M., van Golde L.M., Vaandrager A.B. Inhibition of phosphatidylcholine synthesis induces expression of the endoplasmic reticulum stress and apoptosis-related protein CCAAT/enhancer-binding protein-homologous protein (CHOP/GADD153). Biochem. J. 2003, 369:643-650.
42. Wang M.X., Ren L.M. Growth inhibitory effect and apoptosis induced by extracellular ATP and adenosine on human gastric carcinoma cells: involvement of intracellular uptake of adenosine. Acta Pharmacol. Sin. 2006, 27:1085-1092.
43. Wong D.C., Wong K.T., Lee Y.Y., Morin P.N., Heng C.K., Yap M.G. Transcriptional profiling of apoptotic pathways in batch and fed-batch CHO cell cultures. Biotechnol. Bioeng. 2006, 94:373-382.
44. Wu J.T. Screening for inborn errors of amino acid metabolism. Ann. Clin. Lab. Sci. 1991, 21:123-142.
45. Wyllie A.H., Morris R.G., Smith A.L., Dunlop D. Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis. J. Pathol. 1984, 142(1):67-77.
46. Xaus J., Valledor A.F., Cardo M., Marques L., Beleta J., Palacios J.M., Celada A. Adenosine inhibits macrophage colony-stimulating factor-dependent proliferation of macrophages through the induction of p27kip-1 expression. J. Immunol. 1999, 163:4140-4149.