Investigating the contribution of the active site environment to the slow reaction of hypoxia-inducible factor prolyl hydroxylase domain 2 with oxygen

Biochemical Journal

Volumn 463, Issue 3, 2014, Pages 363-372

  Tarhonskaya, Hanna ^a   Chowdhury, Rasheduzzaman ^a   Leung, Ivanhoe K H ^a   Loik, Nikita D ^a   McCullagh, James S O ^a   Claridge, Timothy D W ^a   Schofield, Christopher J ^a   Flashman, Emily ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Oxygen sensor; Pre steady state kinetics; Prolyl hydroxylase domain 2; Stopped flow; UV visible spectroscopy

Indexed keywords

FERROUS ION; HYPOXIA INDUCIBLE FACTOR; HYPOXIA INDUCIBLE FACTOR PROLINE DIOXYGENASE; OXYGEN; OXYGENASE; 2 OXOGLUTARIC ACID; ALPHA-KETOGLUTARIC ACID; DIVALENT CATION; IRON; ISOQUINOLINE DERIVATIVE; MANGANESE; OLIGOPEPTIDE; PROTEIN BINDING; WATER; ZINC;

ARTICLE; CATALYSIS; CHEMICAL INTERACTION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME SUBSTRATE COMPLEX; HYDROGEN BOND; HYDROXYLATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; METAL BINDING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; SITE DIRECTED MUTAGENESIS; STATIC ELECTRICITY; STEADY STATE; ANTAGONISTS AND INHIBITORS; CHEMISTRY; GENETICS; KINETICS;

CATALYTIC DOMAIN; CATIONS, DIVALENT; HYDROXYLATION; HYPOXIA-INDUCIBLE FACTOR-PROLINE DIOXYGENASES; IRON; ISOQUINOLINES; KETOGLUTARIC ACIDS; KINETICS; MANGANESE; MUTAGENESIS, SITE-DIRECTED; OLIGOPEPTIDES; OXYGEN; PROTEIN BINDING; WATER; ZINC;

EID: 84907816968     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20140779     Document Type: Article

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