Substrate replenishment extends protein synthesis with an in vitro translation system designed to mimic the cytoplasm

Biotechnology and Bioengineering

Volumn 87, Issue 4, 2004, Pages 465-471

  Jewett, Michael C ^a   Swartz, James R ^a  

^a Stanford University   (United States)

Author keywords

Amino acid; Cell free protein synthesis; Combined transcription translation; Cytoplasmic mimicry; Fed batch reaction; Nucleotide

Indexed keywords

BIOMEDICAL ENGINEERING; BIOTECHNOLOGY; CONCENTRATION (PROCESS); DNA; ENERGY ABSORPTION; METABOLISM; RNA; SYNTHESIS (CHEMICAL);

CYTOPLASM; NUCLEOTIDES; PROTEIN SYNTHESIS;

PROTEINS;

ADENOSINE DIPHOSPHATE; ADENOSINE PHOSPHATE; ADENOSINE TRIPHOSPHATE; ALANINE; AMINO ACID; ASPARAGINE; ASPARTIC ACID; BETA GALACTOSIDASE; CHLORAMPHENICOL ACETYLTRANSFERASE; CYSTEINE; CYTIDINE DIPHOSPHATE; CYTIDINE PHOSPHATE; CYTIDINE TRIPHOSPHATE; GLUTAMINE; GUANOSINE DIPHOSPHATE; GUANOSINE PHOSPHATE; GUANOSINE TRIPHOSPHATE; LEUCINE; LUCIFERASE; MACROGOL; MAGNESIUM; NUCLEOTIDE; PLASMID DNA; PUTRESCINE; PYRUVIC ACID; RNA POLYMERASE; SERINE; SPERMIDINE; THREONINE; URIDINE TRIPHOSPHATE;

AMINO ACID ANALYSIS; ARTICLE; CELL FREE SYSTEM; CYTOPLASM; ESCHERICHIA COLI; FED BATCH REACTOR; GENETIC TRANSCRIPTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR MIMICRY; NONHUMAN; PROTEIN SYNTHESIS;

FELIS CATUS;

EID: 4344670572     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20139     Document Type: Article

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