Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system

Journal of Biotechnology

Volumn 164, Issue 2, 2012, Pages 220-231

  Stech, Marlitt ^a   Merk, Helmut ^b   Schenk, Jörg A ^c,d   Stöcklein, Walter F M ^a   Wüstenhagen, Doreen A ^a   Micheel, Burkhard ^c   Duschl, Claus ^a   Bier, Frank F ^a,c   Kubick, Stefan ^a  

^a FRAUNHOFER INSTITUTE FOR BIOMEDICAL ENGINEERING   (Germany)

^b RiNA GmbH   (Germany)

^c UNIVERSITY OF POTSDAM   (Germany)

^d Hybrotec GmbH   (Germany)

Author keywords

Cell free; In vitro translation; Insect lysate; Single chain antibody (scFv); Surface plasmon resonance

Indexed keywords

CELL FREE PROTEIN SYNTHESIS; CELL-FREE; CELL-FREE TRANSLATION SYSTEMS; FUNCTIONAL ANTIBODY FRAGMENTS; IN-VITRO; INSECT LYSATE; POST-TRANSLATIONAL MODIFICATIONS; SINGLE-CHAIN ANTIBODIES;

BIOSYNTHESIS; CELL MEMBRANES; REDOX REACTIONS; SURFACE PLASMON RESONANCE;

ANTIBODIES;

HYBRID PROTEIN; MELITTIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL LYSATE; CELL STRUCTURE; CELL VACUOLE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; FLUORESCENCE ANALYSIS; IN VITRO STUDY; INSECT CELL; MICROSOME; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; BIOREACTORS; BIOTECHNOLOGY; CELL CULTURE TECHNIQUES; CELL-FREE SYSTEM; CULTURE MEDIA, CONDITIONED; MELITTEN; OXIDATION-REDUCTION; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; SF9 CELLS; SINGLE-CHAIN ANTIBODIES; TRANSFECTION;

EUKARYOTA; HEXAPODA;

EID: 84867025898     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.08.020     Document Type: Article

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