High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites

Biochemical and Biophysical Research Communications

Volumn 418, Issue 4, 2012, Pages 652-656

  Ozawa, Kiyoshi ^a,b   Loscha, Karin V ^a   Kuppan, Kekini V ^a   Loh, Choy Theng ^a   Dixon, Nicholas E ^b   Otting, Gottfried ^a  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^b UNIVERSITY OF WOLLONGONG   (Australia)

Author keywords

Aminoacyl tRNA synthetase; Cell free protein synthesis; Escherichia coli; Suppressor tRNA; Unnatural amino acids

Indexed keywords

AMINO ACID TRANSFER RNA LIGASE; DNAB HELICASE; PHENYLALANINE; REAGENT;

ARTICLE; CELL ENGINEERING; CELL FREE SYSTEM; CODON; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; IN VIVO STUDY; METHANOCALDOCOCCUS JANNASCHII; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

ALANINE; AMINO ACYL-TRNA SYNTHETASES; CELL-FREE SYSTEM; CODON, NONSENSE; ESCHERICHIA COLI; GENETIC ENGINEERING; METHANOCOCCUS; NITRILES; PHENYLALANINE; PROTEIN BIOSYNTHESIS; RNA, TRANSFER; SUPPRESSION, GENETIC;

ESCHERICHIA COLI; METHANOCALDOCOCCUS JANNASCHII;

EID: 84857440637     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.01.069     Document Type: Article

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