Cell-free protein synthesis from a release factor 1 deficient escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation

ACS Synthetic Biology

Volumn 3, Issue 6, 2014, Pages 398-409

  Hong, Seok Hoon ^a,b   Ntai, Ioanna ^b   Haimovich, Adrian D ^c   Kelleher, Neil L ^b,d   Isaacs, Farren J ^c   Jewett, Michael C ^a,b,d  

^a Northwestern University   (United States)

^b NORTHWESTERN UNIVERSITY   (United States)

^c YALE UNIVERSITY   (United States)

^d NORTHWESTERN UNIVERSITY   (United States)

Author keywords

cell free protein synthesis; genomically recoded organisms; nonstandard amino acid; PURE translation; release factor 1

Indexed keywords

AMINO ACID; BACTERIAL PROTEIN; BIOPOLYMER; GREEN FLUORESCENT PROTEIN; PHENYLALANINE DERIVATIVE; RELEASE FACTOR 1; UNCLASSIFIED DRUG; 3-(4-ACETYLPHENYL)-2-AMINOPROPANOIC ACID; 4-PROPARGYLOXYPHENYLALANINE; BACTERIAL DNA; ESCHERICHIA COLI PROTEIN; PHENYLALANINE; PRFA PROTEIN, E COLI; STOP CODON; TRANSLATION TERMINATION FACTOR;

ARTICLE; BACTERIAL STRAIN; CELL FREE PROTEIN SYNTHESIS; CELL LYSATE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; STOP CODON; ANALOGS AND DERIVATIVES; CELL FREE SYSTEM; CHEMISTRY; GENE DELETION; GENETICS; PLASMID; PROMOTER REGION; PROTEIN CONFORMATION; SYNTHESIS;

CELL-FREE SYSTEM; CODON, TERMINATOR; DNA, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE DELETION; GREEN FLUORESCENT PROTEINS; PEPTIDE TERMINATION FACTORS; PHENYLALANINE; PLASMIDS; PROMOTER REGIONS, GENETIC; PROTEIN BIOSYNTHESIS; PROTEIN CONFORMATION;

EID: 84896452607     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb400140t     Document Type: Article

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