Complete knockout of the lactate dehydrogenase A gene is lethal in pyruvate dehydrogenase kinase 1, 2, 3 down-regulated CHO cells

Molecular Biotechnology

Volumn 56, Issue 9, 2014, Pages 833-838

  Yip, Shirley S M ^a   Zhou, Meixia ^a   Joly, John ^a   Snedecor, Bradley ^a   Shen, Amy ^a   Crawford, Yongping ^a  

^a GENENTECH INC   (United States)

Author keywords

CHO; Knockout; Lactate; Lactate dehydrogenase (LDHA); Pyruvate dehydrogenase kinase (PDHK); Zinc finger nucleases (ZFN)

Indexed keywords

AMINO ACIDS; BATCH CELL CULTURE; CLONING; DNA SEQUENCES; ENZYMES;

CHO; KNOCKOUT; LACTATE; LACTATE DEHYDROGENASE; PYRUVATE DEHYDROGENASE KINASE; ZINC-FINGER NUCLEASES (ZFN);

GENES;

LACTATE DEHYDROGENASE; LACTATE DEHYDROGENASE ISOENZYME 5; LACTIC ACID; PYRUVATE DEHYDROGENASE KINASE; PYRUVATE DEHYDROGENASE KINASE 1; PYRUVATE DEHYDROGENASE KINASE 2; PYRUVATE DEHYDROGENASE KINASE 3; PYRUVIC ACID; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG; VALINE; PROTEIN SERINE THREONINE KINASE; PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING) KINASE;

ALLELE; ANIMAL CELL; ARTICLE; CELL DEATH; CHO CELL LINE; CONTROLLED STUDY; DOWN REGULATION; ENZYME ACTIVITY; ENZYME REGULATION; FEMALE; GENE SILENCING; HETEROZYGOTE; MOLECULAR CLONING; MUTATION RATE; NONHUMAN; WILD TYPE; ANIMAL; CRICETULUS; CULTURE TECHNIQUE; GENE INACTIVATION; GENETICS; LETHAL GENE; METABOLISM; MUTATION;

ANIMALS; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETULUS; GENE KNOCKOUT TECHNIQUES; GENES, LETHAL; LACTATE DEHYDROGENASES; MUTATION; PROTEIN-SERINE-THREONINE KINASES; PYRUVIC ACID;

EID: 84905972049     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-014-9762-0     Document Type: Article

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