Control of pH in large-scale, free suspension animal cell bioreactors: Alkali addition and pH excursions

Biotechnology and Bioengineering

Volumn 66, Issue 3, 1999, Pages 171-179

  Langheinrich, Christian ^a   Nienow, Alvin W ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Alkali addition; Animal cell bioreactor; Fluid dynamics; PH excursions; Scale up scale down

Indexed keywords

ADDITION REACTIONS; ANIMAL CELL CULTURE; CARBON DIOXIDE; PH; SODIUM COMPOUNDS;

ANIMAL CELL BIOREACTORS;

BIOREACTORS;

CARBON DIOXIDE; OXYGEN; SODIUM CARBONATE;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL CULTURE; CELL DENSITY; CELL PH; NONHUMAN; OXYGEN CONSUMPTION;

ALKALIES; ANIMALS; BIOMEDICAL ENGINEERING; BIOREACTORS; CARBON DIOXIDE; CARBONATES; CELLS, CULTURED; HYDROGEN-ION CONCENTRATION;

EID: 0033458012     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(1999)66:3<171::AID-BIT5>3.0.CO;2-T     Document Type: Article

References (18)

1. Bailey JE, Ollis DF. 1986. Biochemical engineering fundamentals, 2nd edition. New York: McGraw-Hill.
2. Bonarius HPJ, de Gooijer CD, Tramper J, Schmid G. 1995. Determination of the respiration quotient in mammalian cell culture. Biotechnol Bioeng 45:524-535.
3. Brown ME, Birch JR. 1996. The effect of pH on growth and productivity of cell lines producing monoclonal antibodies at large scale. Cytotechnology 17, Suppl 1, Abstr 1.
4. Bujalski W, Nienow AW, Chatwin S, Cooke M. 1987. The dependency on scale of power numbers of Rushton disk turbines. Chem Eng Sci 41:317-326.
5. Cooke M, Middleton JC, Bush J. 1988. Mixing and mass transfer in filamentous fermentations. In: King R, editor. 2nd Int. Conf. on Bioreactor Fluid Dynamics. Amsterdam: Elsevier Applied Science Publishers. p 37-64.
6. Kiss R, Croughan M, Trask J, Polastri G, Groenhout M, Banka A, Paul J, Koning-Bastiaan H. 1994. Mixing time characterization in large-scale mammalian cell bioreactor. AIChE Annual Meeting, November 1994, San Francisco, CA. Paper No 55c.
7. 3 in volume. Food Bioprod. Proc (Trans I Chem E , Part C) 76:107-116.
8. Lin AA, Kimura RR, Miller WM. 1993. Production of tpa in recombinant CHO cells under oxygen-limited condition. Biotechnol Bioeng 42: 339-350.
9. Nienow AW. 1997. Large-scale free suspension animal cell culture: Cell fragility versus media homogeneity. Genet Eng Biotechnol 17: 111-113.
10. Nienow AW. 1998. Hydrodynamics of stirred hioreactors. Appl Mech Rev 51:3-32.
11. Nienow AW, Langheinrich C, Stevenson NC, Emery AN, Clayton TM, Slater NKH. 1996. Homogenisation and oxygen transfer rates in large agitated and sparged animal cell bioreactors: Some implications for growth and production. Cytotechnology 22:87-94.
12. Nienow AW, Drain SM, Boyes AP, Carpenter KJ. 1997. An experimental study to characterise imperfect macromixing in a stirred semi-batch reactors. Ind Eng Chem Res 36:2984-2989.
13. Oh SKW, Nienow AW, Emery AN, Al-Rubeai M. 1992. Further studies of the culture of mouse hybridomas in an agitated hioreactor with and without sparging. J Biotechnol 22:245-270.
14. Okamoto Y, Hishikawa M, Hashimoto K. 1981. Energy dissipation rate distribution in mixing vessels and its effect on liquid-liquid dispersion and solid-liquid mass transfer. Int Chem Eng 21:88-94.
15. Ruszkowski S. 1994. A rational method for measuring blending performance, and comparison of different impeller types. Proc. 8th European Conference on Mixing. I. Chem Eng Symp Ser 136:283-292.
16. Spier RE. 1991. An overview of animal cell biotechnology: The conjoint application of art, science and engineering. In: Animal cell bioreactors. New York: Butterworth-Heinemann. p 3-18.
17. Thieme C. 1993. Sodium carbonates. In: Ullmanns Enzyclopedia of Industrial Chemistry. Weinheim: VCH Publishers. Vol 24, p 299-316.
18. Westerterp KR, van Swaaij WPM, Beenackers AACM. 1987. Chemical reactor design and operation. Chichester: John Wiley and Sons.