Scalable production of highly sensitive nanosensors based on graphene functionalized with a designed G protein-coupled receptor

Nano Letters

Volumn 14, Issue 5, 2014, Pages 2709-2714

  Lerner, Mitchell B ^a,b   Matsunaga, Felipe ^a   Han, Gang Hee ^a   Hong, Sung Ju ^a,c   Xi, Jin ^a   Crook, Alexander ^a   Perez Aguilar, Jose Manuel ^a   Park, Yung Woo ^c   Saven, Jeffery G ^a   Liu, Renyu ^a   Johnson, A T Charlie ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b Naval Command   (United States)

^c Seoul National University   (South Korea)

Author keywords

biosensor; computational protein design; field effect transistor; Graphene; opioid receptor

Indexed keywords

BINDING ENERGY; CHEMICAL STABILITY; FIELD EFFECT TRANSISTORS; GRAPHENE; PROTEINS;

AQUEOUS ENVIRONMENT; CHEMICAL RECOGNITION; COMPUTATIONAL PROTEIN DESIGN; G PROTEIN COUPLED RECEPTORS; GRAPHENE FIELD-EFFECT TRANSISTORS; GRAPHENE TRANSISTORS; OPIOID RECEPTORS; SCALABLE PRODUCTION;

BIOSENSORS;

GRAPHITE; MU OPIATE RECEPTOR; NALTREXONE; WATER;

ANTAGONISTS AND INHIBITORS; CHEMISTRY; GENETIC PROCEDURES; HUMAN; ISOLATION AND PURIFICATION; PROCEDURES;

BIOSENSING TECHNIQUES; GRAPHITE; HUMANS; NALTREXONE; RECEPTORS, OPIOID, MU; WATER;

EID: 84900517619     PISSN: 15306984     EISSN: 15306992     Source Type: Journal    
DOI: 10.1021/nl5006349     Document Type: Article

References (32)

1. Chung, C.; Kim, Y.-K.; Shin, D.; Ryoo, S.-R.; Hong, B. H.; Min, D.-H. Biomedical Applications of Graphene and Graphene Oxide Acc. Chem. Res. 2013, 46, 2211-2224
2. Kuila, T.; Bose, S.; Khanra, P.; Mishra, A. K.; Kim, N. H.; Lee, J. H. Recent Advances in Graphene-Based Biosensors Biosens. Bioelectron. 2011, 26, 4637-4648
3. Kumar, S.; Peltekis, N.; Lee, K.; Kim, H.-Y.; Duesberg, G. S. Reliable Processing of Graphene Using Metal Etchmasks Nanoscale Res. Lett. 2011, 6, 390
4. Lee, Y.; Bae, S.; Jang, H.; Jang, S.; Zhu, S.-E.; Sim, S. H.; Song, Y. I.; Hong, B. H.; Ahn, J.-H. Wafer-Scale Synthesis and Transfer of Graphene Films Nano Lett. 2010, 10, 490-493
5. Levendorf, M. P.; Ruiz-Vargas, C. S.; Garg, S.; Park, J. Transfer-Free Batch Fabrication of Single Layer Graphene Transistors Nano Lett. 2009, 9, 4479-4483
6. Li, X.; Cai, W.; An, J.; Kim, S.; Nah, J.; Yang, D.; Piner, R.; Velamakanni, A.; Jung, I.; Tutuc, E. Large-Area Synthesis of High-Quality and Uniform Graphene Films on Copper Foils Science 2009, 324, 1312-1314
7. Khamis, S. M.; Jones, R. A.; Johnson, A. T. C. Optimized Photolithographic Fabrication Process for Carbon Nanotube Devices AIP Adv. 2011, 1, 022106
8. 2 Nano Lett. 2007, 7, 1643-1648
9. Dan, Y.; Lu, Y.; Kybert, N. J.; Luo, Z.; Johnson, A. T. C. Intrinsic Response of Graphene Vapor Sensors Nano Lett. 2009, 9, 1472-1475
10. Gao, L.; Ren, W.; Xu, H.; Jin, L.; Wang, Z.; Ma, T.; Ma, L.-P.; Zhang, Z. T.; Fu, Q.; Peng, L.-M. Repeated Growth and Bubbling Transfer of Graphene with Millimetre-Size Single Crystal Grains Using Platinum Nat. Commun. 2012, 3, 699
11. Pimenta, M. A.; Dresselhaus, G.; Dresselhaus, M. S.; Cancado, L. G.; Jorio, A.; Saito, R. Studying Disorder in Graphite-Based Systems by Raman Spectroscopy Phys. Chem. Chem. Phys. 2007, 9, 1276-1291
12. King, N.; Hittinger, C. T.; Carroll, S. B. Evolution of Key Cell Signaling and Adhesion Protein Families Predates Animal Origins Science 2003, 301, 361-363
13. Overington, J. P.; Al-Lazikani, B.; Hopkins, A. L. How Many Drug Targets Are There? Nat. Rev. Drug Discovery 2006, 5, 993-996
14. Zhorov, B. S.; Ananthanarayanan, V. S. Homology Models of Mu-Opioid Receptor with Organic and Inorganic Cations at Conserved Aspartates in the Second and Third Transmembrane Domains Arch. Biochem. Biophys. 2000, 375, 31-49
15. Slovic, A. M.; Kono, H.; Lear, J. D.; Saven, J. G.; DeGrado, W. F. Computational Design of Water-Soluble Analogues of the Potassium Channel Kcsa Proc. Natl. Acad. Sci. U. S. A. 2004, 101, 1828-1833
16. Ma, D. J.; Tillman, T. S.; Tang, P.; Meirovitch, E.; Eckenhoff, R.; Carnini, A.; Xu, Y. NMR Studies of a Channel Protein without Membranes: Structure and Dynamics of Water-Solubilized Kcsa Proc. Natl. Acad. Sci. U. S. A. 2008, 105, 16537-16542
17. Cui, T.; Mowrey, D.; Bondarenko, V.; Tillman, T.; Ma, D.; Landrum, E.; Perez-Aguilar, J. M.; He, J.; Wang, W.; Saven, J. G. NMR Structure and Dynamics of a Designed Water-Soluble Transmembrane Domain of Nicotinic Acetylcholine Receptor Biochim. Biophys. Acta, Biomembr. 2012, 1818, 617-626
18. Perez-Aguilar, J. M.; Saven, J. G. Computational Design of Membrane Proteins Structure 2012, 20, 5-14
19. Perez-Aguilar, J. M.; Xi, J.; Matsunaga, F.; Cui, X.; Selling, B.; Saven, J. G.; Liu, R. A Computationally Designed Water-Soluble Variant of a G-Protein-Coupled Receptor: The Human Mu Opioid Receptor PLoS One 2013, 8, e66009
20. Lu, Y.; Lerner, M. B.; Qi, Z. J.; Mitala, J. J.; Hsien Lim, J.; Discher, B. M.; Johnson, A. T. C. Graphene-Protein Bioelectronic Devices with Wavelength-Dependent Photoresponse Appl. Phys. Lett. 2012, 100, 033110
21. Eckmann, A.; Felten, A.; Mishchenko, A.; Britnell, L.; Krupke, R.; Novoselov, K. S.; Casiraghi, C. Probing the Nature of Defects in Graphene by Raman Spectroscopy Nano Lett. 2012, 12, 3925-3930
22. Manglik, A.; Kruse, A. C.; Kobilka, T. S.; Thian, F. S.; Mathiesen, J. M.; Sunahara, R. K.; Pardo, L.; Weis, W. I.; Kobilka, B. K.; Granier, S. Crystal Structure of the Mu-Opioid Receptor Bound to a Morphinan Antagonist Nature 2012, 485, 321-326
23. Lerner, M. B.; Resczenski, J. M.; Amin, A.; Johnson, R. R.; Goldsmith, J. I.; Johnson, A. T. C. Toward Quantifying the Electrostatic Transduction Mechanism in Carbon Nanotube Molecular Sensors J. Am. Chem. Soc. 2012, 134, 14318-14321
24. Weiss, J. N. The Hill Equation Revisited: Uses and Misuses FASEB J. 1997, 11, 835-841
25. Emmerson, P. J.; Liu, M. R.; Woods, J. H.; Medzihradsky, F. Binding Affinity and Selectivity of Opioids at Mu, Delta and Kappa Receptors in Monkey Brain Membranes J. Pharmacol. Exp. Ther. 1994, 271, 1630-1637
26. Lerner, M. B.; D'Souza, J.; Pazina, T.; Dailey, J.; Goldsmith, B. R.; Robinson, M. K.; Johnson, A. T. C. Hybrids of a Genetically Engineered Antibody and a Carbon Nanotube Transistor for Detection of Prostate Cancer Biomarkers ACS Nano 2012, 6, 5143-5149
27. Cox, R. F.; Collins, M. A. The Effects of Benzodiazepines on Human Opioid Receptor Binding and Function Anesth. Analg. 2001, 93, 354-358
28. Carlson, K. E.; Choi, I.; Gee, A.; Katzenellenbogen, B. S.; Katzenellenbogen, J. A. Altered Ligand Binding Properties and Enhanced Stability of a Constitutively Active Estrogen Receptor: Evidence That an Open Pocket Conformation Is Required for Ligand Interaction Biochemistry 1997, 36, 14897-14905
29. Das, A.; Pisana, S.; Chakraborty, B.; Piscanec, S.; Saha, S. K.; Waghmare, U. V.; Novoselov, K. S.; Krishnamurthy, H. R.; Geim, A. K.; Ferrari, A. C. Monitoring Dopants by Raman Scattering in an Electrochemically Top-Gated Graphene Transistor Nat. Nanotechnol. 2008, 3, 210-215
30. Dresselhaus, M. S.; Dresselhaus, G.; Pimenta, M. A.; Malard, L. M. Raman Spectroscopy in Graphene Phys. Rep. 2009, 473, 51-87
31. Kybert, N. J.; Han, G. H.; Lerner, M. B.; Esfandiar, A.; Johnson, A. T. C. Scalable Arrays of DNA-Decorated Graphene Chemical Vapor Sensors Nano Res. 2013, 7, 95-103
32. Saby, C.; Ortiz, B.; Champagne, G. Y.; Bélanger, D. Electrochemical Modification of Glassy Carbon Electrode Using Aromatic Diazonium Salts. 1. Blocking Effect of 4-Nitrophenyl and 4-Carboxyphenyl Groups Langmuir 1997, 13, 6805-6813